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Cellulosimicrobium cellulans and method for producing trehalose through penetration fermentation of cellulosimicrobium cellulans

A technology of trehalose and chemical fiber, applied in the field of microbial fermentation to produce trehalose, can solve the problems of complex product separation process, high production cost, lack of information, etc., and achieve the effects of good non-specific protection, reduced production cost, and broad application prospects.

Active Publication Date: 2012-08-15
安徽民祯生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzyme conversion method has the advantages of good specificity and high conversion rate, but the enzymes used need to be refined. Due to the high cost of enzyme production and the complicated product separation process, the production cost is high
Most of the currently applied enzymatic conversion systems produce trehalose from maltooligosaccharides, and there is little information on the efficient production of trehalose from glucose (one of the useful starting materials for industrial production of organic substances).

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] In this example, the method for producing trehalose by osmotic fermentation using fibroblast microbacteria includes culture of fibroblast microbacteria, osmotic fermentation, synthesis of trehalose, and extraction and separation of trehalose. The specific operation steps are as follows:

[0015] (1) Cultivation of fibrosis cellulans: the cellulosimicrobium cellulans S32 bacterial strain whose strain preservation number is CGMCC No.5841 is used for activation and cultivation, and the inoculation amount of 5% of the culture medium volume is transferred to a fermentation tank. In the 5L fermenter of the culture medium, the liquid volume is 3.5L, the culture temperature is 30°C, the air flow rate is 4L / min, the rotation speed is 300r / min, and the culture time is 30h.

[0016] The components of the above-mentioned fermentation medium are as follows: glucose 20, yeast powder 5, peptone 5, corn steep liquor 10, MgSO 4 1. KH 2 PO 4 1 and CaCO 3 7 (g / L).

[0017] (2) Osm...

Embodiment 2

[0020] (1) Fibrosis cellulomicrobacteria culture: get the fibrosis cellulomicrobacteria preserved bacterial classification activation culture, transfer in the 5L fermenter that fermentation medium is placed by the 5% inoculum size of culture medium volume, liquid capacity 3.5L, The culture temperature is 30°C, the air flow rate is 4L / min, the rotation speed is 300r / min, and the culture time is 30h.

[0021] The fermentation medium is the same as in Example 1, and the components are in grams per liter of medium: glucose 20, yeast powder 5, peptone 5, corn steep liquor 10, MgSO 4 1. KH 2 PO 4 1 and CaCO 3 7 (g / L).

[0022](2) Osmotic fermentation: add 17.5g (NH 4 ) 2 SO 4 and 210g glucose, and cultured for another 7h.

[0023] (3) Separation and purification of trehalose: the fermentation broth was centrifuged or filtered to remove strain cells to obtain a solution with a trehalose content of 32 g / L. Use an ultrafiltration membrane with a relative molecular weight cut...

Embodiment 3

[0025] (1) Fibrosis cellulomicrobacteria culture: get the fibrosis cellulomicrobacteria preserved bacterial classification activation culture, transfer in the 5L fermenter that fermentation medium is placed by the 5% inoculum size of culture medium volume, liquid capacity 3.5L, The culture temperature is 30°C, the air flow rate is 4L / min, the rotation speed is 300r / min, and the culture time is 30h. The fermentation medium is the same as in Example 1, and the components are in grams per liter of medium: glucose 20, yeast powder 5, peptone 5, corn steep liquor 10, MgSO 4 1. KH 2 PO 4 1 and CaCO 3 7 (g / L).

[0026] (2) Osmotic fermentation: add 35g (NH 4 ) 2 SO 4 and 210g glucose, and cultured for another 7h.

[0027] (3) Separation and purification of trehalose: the fermentation broth was centrifuged or filtered to remove strain cells to obtain a solution with a trehalose content of 31 g / L. Use an ultrafiltration membrane with a relative molecular weight cut-off of 10...

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PUM

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Abstract

The invention discloses cellulosimicrobium cellulans and a method for producing trehalose through the penetration fermentation of the cellulosimicrobium cellulans. The new strain is named as the cellulosimicrobium cellulans S32, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) N0.5841. According to the method, glucose serves as a substrate; the penetration fermentation is performed on the cellulosimicrobium cellulans S32 after the cellulosimicrobium cellulans S32 is cultured; after being subjected to stress, cellulosimicrobium cellulans cells generate more trehalose synthetase systems and become permeability cells; and a great deal of intracellular trehalose synthetase systems are synthetized into extracellular trehalose. The conversion rate from the glucose to the trehalose reaches 60%, carbohydrate by-products are avoided, the product is easy to separate, the production processes are intensive, the equipment investment is less, and the production cost is lower. Due to the good non-specificity protective effect on large biological molecules, the prepared trehalose has a wide application prospect in the fields of medicaments, cosmetics, foods and the like.

Description

technical field [0001] The invention belongs to the technical field of methods for producing trehalose by microbial fermentation, and in particular relates to a method for producing extracellular trehalose by osmotic fermentation of fibrotic microbacteria using glucose as a substrate. Background technique [0002] According to "Acta Biochemistry and Biophysics" (Crowe et al, Biochim.Biophys.Acta, 1984, 769: 151-159) and "Food Chemistry" (Ohtani et al, Food Chem., 1994, 2: 91-95) Introduction, trehalose (α-D-glucopyranosyl-α-D-glucopyranoside) is a non-reducing glucoside widely distributed in plants, insects, fungi and bacteria, and this disaccharide can be used as an energy source A storage form and protectant against environmental stresses such as drying or freezing. Since there is no reducing end in its molecular structure, trehalose not only shows resistance to extreme temperature, pH and Maillard reaction, but also has high water-holding activity, so trehalose has the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/12C12R1/01
Inventor 宋建民
Owner 安徽民祯生物工程有限公司
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