Lactic dehydrogenase detection kit and preparation method thereof

The technology of lactate dehydrogenase and detection kit is applied in the field of detection of lactate dehydrogenase content in the human body, which can solve the problems of inability to guarantee the stability of reagents, troublesome detection work, influence on results, etc., and achieves great clinical practical value and is convenient for clinical use. Useful, high-precision effects
CN102766677AInactive Publication Date: 2012-11-07WUHAN LIFE ORIGIN BIOTECH LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
WUHAN LIFE ORIGIN BIOTECH LTD
Publication Date
2012-11-07
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses a lactic dehydrogenase detection kit and a preparation method of the kit. The kit disclosed by the invention consists of a reagent I and a reagent II which are independent each other, wherein the components of the reagent I comprise a biobuffer, a metal ion complex, a lactic dehydrogenase reactive substrate, a surfactant, a lactic dehydrogenase activity activating agent, a preservative and water; and the components of the reagent II comprise a biobuffer, a nicotinamide adenine dinucleotide oxidation state, a surfactant, a preservative and water. The detection kit disclosed by the invention adopts a dual reagent mode to effectively avoid the interference of the nonspecific reaction and synchronously furthest reduce the interference of the sample turbidity, thereby guaranteeing the stable and reliable measurement result; and the detection kit has the advantages of good stability, high precision, wide linear testing range, good repeatability, and strong anti-interference performance and the like. In addition, the detection kit disclosed by the invention does not need to pre-dilute the sample in the detecting process, thereby being convenient for clinical use, simple and fast to operate, and suitable for an automatic biochemical analyzer.
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Description

technical field

[0001] The present invention relates to a detection kit for enzyme content in the human body, in particular to a lactate dehydrogenase assay kit and a preparation method thereof, and the present invention also relates to the use of the lactate dehydrogenase assay kit in detecting lactate dehydrogenase in the human body The method for using the content belongs to the detection field of lactate dehydrogenase content in human body. Background technique

[0002] Lactate dehydrogenase (LDH) is a glycolytic enzyme that exists in the cytoplasm of all tissue cells in the body, among which the kidney has a higher content. Lactate dehydrogenase is an enzyme that catalyzes the dehydrogenation of lactate to pyruvate, and is present in almost all tissues. When the human body has acute hepatitis, acute myocardial infarction, congestive heart failure, muscular dystrophy, polymyositis, malignant tumor, liver cancer, lung cancer, acute leukemia, lymphosarcoma, pernicious ane...

Claims

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