Lactic dehydrogenase detection kit and preparation method thereof

The technology of lactate dehydrogenase and detection kit is applied in the field of detection of lactate dehydrogenase content in the human body, which can solve the problems of inability to guarantee the stability of reagents, troublesome detection work, influence on results, etc., and achieves great clinical practical value and is convenient for clinical use. Useful, high-precision effects

Inactive Publication Date: 2012-11-07
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A method for measuring lactate dehydrogenase in serum by rate method has been reported in the literature (Yan Xingwei, Han Shulu, etc.; rate method for determining lactate dehydrogenase in serum, Shanghai Journal of Medical Laboratory, 1991), although the method is relatively simple, but due to the The coenzyme I so...

Method used

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  • Lactic dehydrogenase detection kit and preparation method thereof
  • Lactic dehydrogenase detection kit and preparation method thereof
  • Lactic dehydrogenase detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 lactate dehydrogenase detection kit:

[0028] 1. Preparation of reagent Ⅰ:

[0029] Take each component according to the stated dosage:

[0030]

[0031] Dissolve the above-mentioned components in distilled water or double-distilled water, set the volume to 1 liter, keep it sealed, and keep it for later use.

[0032] 2. Preparation of Reagent II:

[0033] Take each component according to the stated dosage:

[0034]

[0035] Dissolve the above-mentioned components in distilled water or double-distilled water, set the volume to 1 liter, keep it sealed, and keep it for later use.

Embodiment 2

[0036] The preparation of embodiment 2 lactate dehydrogenase detection kit

[0037] 1. Preparation of reagent Ⅰ:

[0038] Take each component according to the stated dosage:

[0039]

[0040] Dissolve the above-mentioned components in distilled water or double-distilled water, set the volume to 1 liter, keep it sealed, and keep it for later use.

[0041] 2. Preparation of Reagent II:

[0042] Take each component according to the stated dosage:

[0043]

[0044]Dissolve the above-mentioned components in distilled water or double-distilled water, set the volume to 1 liter, keep it sealed, and keep it for later use.

Embodiment 3

[0045] The preparation of embodiment 3 lactate dehydrogenase detection kit

[0046] 1. Preparation of reagent Ⅰ:

[0047] Take each component according to the stated dosage:

[0048]

[0049] Dissolve the above-mentioned components in distilled water or double-distilled water, set the volume to 1 liter, keep it sealed, and keep it for later use.

[0050] 2. Preparation of Reagent II:

[0051] Take each component according to the stated dosage:

[0052]

[0053] Dissolve the above-mentioned components in distilled water or double-distilled water, set the volume to 1 liter, keep it sealed, and keep it for later use.

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Abstract

The invention discloses a lactic dehydrogenase detection kit and a preparation method of the kit. The kit disclosed by the invention consists of a reagent I and a reagent II which are independent each other, wherein the components of the reagent I comprise a biobuffer, a metal ion complex, a lactic dehydrogenase reactive substrate, a surfactant, a lactic dehydrogenase activity activating agent, a preservative and water; and the components of the reagent II comprise a biobuffer, a nicotinamide adenine dinucleotide oxidation state, a surfactant, a preservative and water. The detection kit disclosed by the invention adopts a dual reagent mode to effectively avoid the interference of the nonspecific reaction and synchronously furthest reduce the interference of the sample turbidity, thereby guaranteeing the stable and reliable measurement result; and the detection kit has the advantages of good stability, high precision, wide linear testing range, good repeatability, and strong anti-interference performance and the like. In addition, the detection kit disclosed by the invention does not need to pre-dilute the sample in the detecting process, thereby being convenient for clinical use, simple and fast to operate, and suitable for an automatic biochemical analyzer.

Description

technical field [0001] The present invention relates to a detection kit for enzyme content in the human body, in particular to a lactate dehydrogenase assay kit and a preparation method thereof, and the present invention also relates to the use of the lactate dehydrogenase assay kit in detecting lactate dehydrogenase in the human body The method for using the content belongs to the detection field of lactate dehydrogenase content in human body. Background technique [0002] Lactate dehydrogenase (LDH) is a glycolytic enzyme that exists in the cytoplasm of all tissue cells in the body, among which the kidney has a higher content. Lactate dehydrogenase is an enzyme that catalyzes the dehydrogenation of lactate to pyruvate, and is present in almost all tissues. When the human body has acute hepatitis, acute myocardial infarction, congestive heart failure, muscular dystrophy, polymyositis, malignant tumor, liver cancer, lung cancer, acute leukemia, lymphosarcoma, pernicious ane...

Claims

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Application Information

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IPC IPC(8): C12Q1/32
Inventor 华权高许可舒芹
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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