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Metal-resin complex and use thereof

A complex and resin technology, applied in the field of test strips for lateral flow chromatography, can solve the problems of difficult antigens or antibodies, unclear effects, insufficient visibility, etc., to achieve suppression of non-specific reactions, excellent detection sensitivity, and visual excellent recognition effect

Pending Publication Date: 2019-08-16
NIPPON STEEL CHEM &MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the color tone of the colloidal particles is determined by the particle size and preparation conditions, so there is a problem that it is difficult to obtain the desired vivid deep color tone, that is, the visibility is insufficient.
[0007] In addition, the above-mentioned colored latex particles have the following problems: the coloring effect by the pigment is low, and the visual judgment is insufficient.
Furthermore, if the coloring amount of the pigment is increased in order to solve the above-mentioned problems, there is a problem that the pigment covers the surface of the latex, and the original surface state of the latex particles is damaged, so it is difficult to bind the antigen or the antibody.
In addition, there are also the following problems: clogging in the pores of chromatographic media such as membrane filters; non-specific aggregation of latex particles; or deep coloring by adding coloring materials of pigments, but not necessarily related to performance improvement
However, the detailed structure and physical properties of the composite fine particles have not been studied, and the effect on specific applications such as reagents for immunological measurement is unknown.
[0016] Based on the above, latex particles bonded or coated with gold nanoparticles are expected to be used as reagents for immunological assays, but the conventional technology has insufficient durability or visibility.
In addition, even if the visibility is high, applicable applications and usage environments are limited

Method used

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  • Metal-resin complex and use thereof
  • Metal-resin complex and use thereof
  • Metal-resin complex and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0229]

[0230] After adding 54 g of pure water to B-1 (91.5 g), 400 mM chloroplatinic acid aqueous solution (100 g) was added, and it stirred at 30 degreeC for 3 hours. After the mixed solution was left to stand for 24 hours, A-1 was precipitated by centrifugation (3000 rpm, 30 minutes), and the supernatant was removed to remove excess chloroplatinic acid. Thereafter, the concentration was adjusted to obtain a 5% by weight platinum ion-adsorbing resin particle dispersion C-1.

[0231] Next, C-1 (20.6 g) was added to 1392 g of pure water, and while stirring at 3° C., 132 mM dimethylamine borane aqueous solution (40 g) was added dropwise over 20 minutes, and stirred at 3° C. for 1 hour And stirring at room temperature for 3 hours, the platinum-resin composite particle D-1 with an average particle diameter of 381 nm was obtained. D-1 was concentrated by centrifugation, purified by dialysis, and the concentration was adjusted to obtain 1 wt % platinum-resin composite particle ...

Embodiment 2

[0234]

[0235] Using B-2 instead of B-1, in the same manner as in Example 1, 5 wt% of platinum ion-adsorbing resin particle dispersion C-2, platinum-resin composite particles D-2, 1 wt% Platinum-resin composite particle dispersion E-2 and platinum-resin composite particle F-2.

[0236] The average particle diameter of D-2 is 461 nm, and the absorbance of F-2 is 1.77. In addition, the average particle diameter of the platinum particles in F-2 was 5.0 nm, and the supported amount of platinum was 37.6 wt%. Table 1 shows the results of the zeta potential measurement of F-2.

[0237] In the above resin-platinum composite F-2, the platinum particles include contained platinum particles completely contained in the resin particles, partially exposed platinum particles having a part embedded in the resin particle and a part exposed outside the resin particle, And the surface-adsorbed platinum particles adsorbed on the surface of the resin particles, at least a part of the platinum...

Embodiment 3

[0239]

[0240] Using B-3 instead of B-1, in the same manner as in Example 1, 5 wt% of platinum ion-adsorbing resin particle dispersion C-3, platinum-resin composite particles D-3, 1 wt% Platinum-resin composite particle dispersion E-3 and platinum-resin composite particle F-3.

[0241] The average particle diameter of D-3 is 454 nm, and the absorbance of F-3 is 1.45. In addition, the average particle diameter of the platinum particles in F-3 was 3.8 nm, and the supported amount of platinum was 37.7 wt%. Table 1 shows the results of the zeta potential measurement of F-3.

[0242] In the above resin-platinum composite F-3, the platinum particles include contained platinum particles completely contained in the resin particles, partially exposed platinum particles having a part embedded in the resin particle and a part exposed outside the resin particle, And the surface-adsorbed platinum particles adsorbed on the surface of the resin particles, at least a part of the platinum...

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Abstract

Provided is a metal-resin complex that has a structure in which a plurality of metal particles are fixed to resin particles and, in the range of pH 3 to pH 10, the maximum value of the zeta potentialis at least 5 mV and the minimum value is -5 mV or less. In this metal-resin complex, it is preferable that, in the range of pH 3 to pH 10, the difference between the maximum value and the minimum value of the zeta potential is at least 20 mV; and it is further preferable that the zero point of charge of the zeta potential is in the range of pH 3.5 to pH 9.0. This metal-resin complex can be used in immunoassays and in immunoassay reagents as a labeled substance by making antigens or antibodies adhere to the surface of the metal- resin complex.

Description

technical field [0001] The present invention relates to a metal-resin complex that can be suitably used in, for example, an immunological assay, a labeling substance using the same, an immunological assay, a reagent for an immunological assay, a method for measuring an analyte, Analyte determination kits and test strips for lateral flow chromatography. Background technique [0002] There are countless kinds of chemical substances in the living body, so the technology for qualitative and quantitative analysis of specific trace components in the living body is extremely important. In the fields of medical treatment, pharmaceuticals, health food, biotechnology, and the environment, drugs and foods that act only on specific parts (chemical substances) in living organisms, analysis devices and diagnostic agents that detect slight changes in living organisms, etc. The technologies are developed in tandem. [0003] As one of said analytical techniques, there is immunoassay. It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/41G01N33/553
CPCG01N21/41G01N33/543G01N33/553G01N33/545G01N33/585B82Y30/00B82Y40/00C08J7/12C08J2300/20C08K3/08C08K2003/0831C08K2201/005G01N33/54313
Inventor 榎本靖松村康史
Owner NIPPON STEEL CHEM &MATERIAL CO LTD
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