Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dry chemical method rapid diagnostic reagent strip for testing content of alcohol in saliva and preparation method thereof

A rapid diagnostic and chemical technology, applied in material analysis, biological testing, and chemical reaction of materials by observing the impact on chemical indicators, etc. Positive and other problems, to avoid false positives, avoid false positives, and prevent reaction discoloration

Active Publication Date: 2015-05-13
ZHEJIANG ORIENT GENE BIOTECH
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of detector can adopt fuel cell type, gas sensitive semiconductor element type, electrochemical and infrared absorption sensor type, etc. The price of this kind of detector is different, and the detection effect is different. Some are very expensive and not easy to carry.
According to our country's national conditions, it is impossible for every traffic policeman to have a mobile phone, and this kind of detector must be checked and maintained by professionals from time to time in order to measure accurately. At the same time, there are hygiene problems during use.
[0005] (2) blood alcohol content test method, which adopts headspace gas chromatograph to test, at present, this test method is a statutory test method, and the test result of this test method is accurate and reliable, but the disadvantage is that it is only applicable to laboratory professionals. And the test results take a long time, and mass screening cannot be performed
However, the test paper prepared by this method is prone to false positives, and the stability needs to be improved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The preparation of reaction solution A: reaction solution A comprises the material obtained after weighing the following materials and mixing uniformly:

[0068] 1L of water;

[0069] GOOD buffer 20~200mM;

[0070] EDTA 10~100mM;

[0071] Polyvinylpyrrolidone is 2~20g

[0072] Bovine serum albumin: 1-10g

[0073] 2-Mercaptothreitol 20~100mg;

[0074] Alcohol oxidase 1~10KU;

[0075] Peroxidase 10~40KU;

[0076] N-ethyl-N-(2-hydroxy-3-sulfopropyl)m-toluidine sodium salt 10~50mM

[0077] The first soaking and drying: process the 31ET filter paper produced by Whatman according to the size of the reagent strip, then soak it in the reaction solution A, take it out and dry it at 60°C for 50 minutes;

[0078] The preparation of reaction solution B: reaction solution B comprises the material that weighs following material and mixes homogeneously to obtain:

[0079] Acetone 400mL;

[0080] Ethyl cellulose 1~10g

[0081] Polyethylene glycol 6000 5~10g;

[0082] 2-Merc...

Embodiment 2

[0093] The preparation of reaction solution A: reaction solution A comprises the material obtained after weighing the following materials and mixing uniformly:

[0094] 1L of water;

[0095] GOOD buffer 20~200mM;

[0096] EDTA 10~100mM;

[0097] Polyvinylpyrrolidone is 1~18g

[0098] Bovine serum albumin is 2 ~ 5g

[0099] Quinoline 20~100mg;

[0100] Alcohol oxidase 1~10KU;

[0101] Peroxidase 10~40KU;

[0102] The first soaking and drying: process the 31ET filter paper produced by Whatman according to the size of the reagent strip, then soak it in the reaction solution A, take it out and dry it at 50°C for 45 minutes;

[0103] The preparation of reaction solution B: reaction solution B comprises the material that weighs following material and mixes homogeneously to obtain:

[0104] Acetone 400mL;

[0105] Ethyl cellulose 1~10g

[0106] Polyethylene glycol 6000 5~10g;

[0107] 3,3,5,5-Tetramethylbenzidine 50~200mg;

[0108] 8-hydroxyquinoline-5-sulfonic acid 20~1...

Embodiment 3

[0116] The preparation of reaction solution A: reaction solution A comprises the material obtained after weighing the following materials and mixing uniformly:

[0117] 1L of water;

[0118] GOOD buffer 20~200mM;

[0119] EDTA 10~100mM;

[0120] 8-15g of polyvinylpyrrolidone

[0121] Bovine serum albumin is 4-8g

[0122] Thiazole 20~100mg;

[0123] Alcohol oxidase 1~10KU;

[0124] Peroxidase 10~40KU;

[0125] The first soaking and drying: process the 31ET filter paper produced by Whatman according to the size of the reagent strip, then soak it in the reaction solution A, take it out and dry it at 70°C for 55 minutes;

[0126] The preparation of reaction solution B: reaction solution B comprises the material that weighs following material and mixes homogeneously to obtain:

[0127] Acetone 400mL;

[0128] Ethyl cellulose 1~10g

[0129] Polyethylene glycol 6000 5~10g;

[0130] 3-Hydroxy-2,4,6-tribromobenzoic acid 50~200mg;

[0131] 8-hydroxyquinoline-5-sulfonic acid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rapid detection reagent strip and a preparation method thereof and in particular relates to a dry chemical method rapid diagnostic reagent strip for testing content of alcohol in saliva and a preparation method thereof. The dry chemical method rapid diagnostic reagent strip comprises a support for supporting a reactant system, and a piece of filtering paper for absorbing the reactants, wherein the reactants comprise a color developing agent, ethanol oxidase and / or peroxidase, nitrogen heterocyclic compounds and additives; and the additives comprise one or more than two of bovine serum albumin, polyvinylpyrrolidone or ethyl cellulose. The preparation method comprises the steps of preparing a reaction liquid A, steeping and drying for the first time, preparing a reaction liquid B, steeping and drying for the second time and assembling the reagent strip. The reagent strip can effectively avoid the false positive phenomenon and is high in stability.

Description

technical field [0001] The invention relates to a rapid detection reagent strip and a manufacturing method thereof, in particular to a dry chemical rapid diagnostic reagent strip for testing the alcohol content in saliva and a preparation method thereof. Background technique [0002] Alcohol detection has a wide range of uses, including the detection of alcohol poisoning patients in hospitals, and the monitoring of whether workers in flammable and explosive places such as mines, organic solvent production workshops or warehouses have drunk operations, etc. However, the most important application of alcohol detection is the determination of the alcohol concentration in the body of a motor vehicle driver. Drunk driving is a worldwide safety problem. According to foreign reports, the proportion of traffic fatal accidents related to drunk driving is very high every year. According to the World Health Organization (WHO), more than 80% of traffic accidents in traffic accidents a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N33/52
Inventor 冯海英
Owner ZHEJIANG ORIENT GENE BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com