38 results about "Severe fever with thrombocytopenia syndrome virus" patented technology

The present invention relates to an antibody which specifically binds to the envelope glycoprotein of severe fever with thrombocytopenia syndrome virus (SFTSV), the pathogen of severe fever with thrombocytopenia syndrome (SFTS), and is used in order to effectively detect or diagnosis SFTSV and treat SFTS.

The invention relates to a reagent kit and application thereof, particularly relates to an isothermal amplification reagent kit for detecting fever and thrombocytopenia syndrome viruses and application of the isothermal amplification reagent kit, and belongs to the field of biotechnologies. The isothermal amplification reagent kit for detecting the fever and thrombocytopenia syndrome viruses contains oligonucleotide and comprises RNA (ribonucleic acid) extraction reagents (1), isothermal amplification reaction liquid (2), positive control templates (3) and negative control components (4). The isothermal amplification reaction liquid (1) comprises 1*Bst Buffer, betaine, MgSO<4>, dNTPs, RNase inhibitors, Bst DNA (deoxyribonucleic acid) polymerase, AMV reverse transcriptase, sterile deionized water, upstream and downstream strand displacement primers, upstream and downstream probes and a cross amplification primer; the positive control templates (3) are in-vitro transcription products of M gene segments of the fever and thrombocytopenia syndrome viruses; the negative control components (4) are sterile deionized water. The isothermal amplification reagent kit and the application have the advantages that the isothermal amplification reagent kit is high in detection sensitivity, specificity and repeatability, simple in operation step and short in reaction time, and has a broad application prospect, integral reaction procedures can be carried out at the same temperatures, the fever and thrombocytopenia syndrome viruses can be detected only by the aid of a single isothermal instrument, and the like.

The invention relates to a severe fever with thrombocytopenia syndrome virus (SFTSV), an entire gene sequence represented by Hubei isolate HB29, amino acid sequences of coding proteins and application. The entire gene sequence of the virus is subjected to homology analysis. The virus belongs to bunyaviridae and comprises three gene segments, namely, L, M and S which represent polymerase and glycoprotein (Gn and Gc), nucleoprotein (NP) and non-structural proteins (NSs) of the virus respectively, and the three segments are all positioned on the branch of phlebovirus but farther from other viruses of phlebovirus. The entire gene sequence and the coding proteins of the virus can be used for developing drugs, vaccines or diagnostic reagents for preventing and treating the epidemic diseases caused by the SFTSV.

The invention discloses an application of a compound MLN4924 in preparation of a bunyaviridae phlebovirus virus inhibitor. The compound MLN4924 has a structure shown in the formula (I) (shown in the specification), and experiments prove that the compound MLN4924 can inhibit severe fever with thrombocytopenia syndrome viruses or Sicilian phlebotomus fever viruses in bunyaviridae phlebovirus viruses. According to the invention, ubiquitination degradation of RIG-I mediated by SFTSV NSs is inhibited, so that a host cell activates an interferon antiviral pathway during virus infection, and replication and proliferation of viruses are obviously inhibited.

The invention discloses application of rs1800818 to detection of severe fever with thrombocytopenia syndrome (FTLS) caused by severe fever with thrombocytopenia syndrome virus (FTLSV). The technical scheme to be protected is about application of substances for detecting the polymorphism or the genotype of rs1800818 in a personal genome to preparation of a product for screening FTLS and application of substances for detecting the polymorphism or the genotype of rs1800818 in a personal genome to preparation of a product for forecasting the condition of an FTLS patient. The substances for detecting the polymorphism or the genotype of rs1800818 can be combined with other substances (such as a substance for detecting other single nucleotide polymorphism or genotype related to FTLS) to prepare a product for screening FTLS patients or forecasting the conditions of FTLS patients.

The invention relates to preparation and application of an enzyme-linked immunosorbent assay (ELISA) kit for detecting novel bunyavirus antigen of feverwith thrombocytopenia associated syndrome. According to the kit, a domestic rabbit and a mouse are immunized by using severe febrile and thrombocytopenicyndrome virus, called novel bunyavirus JS-2007-001 for short, to obtain antiserum. An enzyme-linked reaction plate is coated by using rabbit antibody, mouse antibody is coupled with horse radish peroxidase (HRP), and auxiliary reagents such as an antigen quantitative reference and an enzyme substrate TMB (Tetramethylbenzidine) color developing agent are matched to form the kit.