RT-LAMP based primer set and kit for detecting SFTSV

Abstract

The invention discloses RT-LAMP based oligonucleotide and a kit for rapidly detecting severe fever with thrombocytopenia syndrome virus (SFTSV). The kit comprises outer primers (F3 and B3), inner primers (FIP and BIP), and loop primers (Loop F and Loop B). The nucleotide sequence of the outer primer F3 is represented by SEQ ID No.1; the nucleotide sequence of the outer primer B3 is represented bySEQ ID No.2; the nucleotide sequence of the inner primer FIP is represented by SEQ ID No.3; the nucleotide sequence of the inner primer BIP is represented by SEQ ID No.4; the nucleotide sequence of the loop primer Loop F is represented by SEQ ID No.5, and the nucleotide sequence of the loop primer Loop B is represented by SEQ ID No.6. The provided kit has the advantages of simple operation, high sensitivity, good specificity, low cost, rapid reaction, and easily observed results, and can rapidly detect SFTSV.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to an oligonucleotide and a kit for rapidly detecting fever with thrombocytopenia syndrome virus based on RT-LAMP. Background technique [0002] Fever with thrombocytopenia syndrome (Severe fever with thrombocytopenia syndrome, SFTS) is a new type of viral hemorrhagic fever with fever and thrombocytopenia as the main clinical features discovered in my country in recent years, mainly distributed in the mountainous and hilly areas of central and eastern my country zone. At present, 16 provinces in my country have reported cases of fever with thrombocytopenia syndrome. Tick ​​bites are the main route of transmission, and direct contact with blood and bloody secretions of patients can also infect the disease and lead to cluster outbreaks. The pathogen of the disease is a new type of Bunyaviridae and Phlebovirus, which is a segmented single-stranded negative-sense RNA virus,...

AI Technical Summary

Problems solved by technology

However, because immunological methods often cause false positives, cell culture methods have high cost, long culture time, high biological safety requirements, complex operation techniques, PCR technology requires sophisticated and expensive instruments, and high requirements for operators. Difficult to promote at the grassroots level

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