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Application of rs1800818 to detection of severe fever with thrombocytopenia syndrome caused by severe fever with thrombocytopenia syndrome virus

A platelet, syndrome technology, applied in the field of biomedicine

Active Publication Date: 2015-07-22
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no effective vaccine and treatment for the disease

Method used

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  • Application of rs1800818 to detection of severe fever with thrombocytopenia syndrome caused by severe fever with thrombocytopenia syndrome virus
  • Application of rs1800818 to detection of severe fever with thrombocytopenia syndrome caused by severe fever with thrombocytopenia syndrome virus
  • Application of rs1800818 to detection of severe fever with thrombocytopenia syndrome caused by severe fever with thrombocytopenia syndrome virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Establishment of the method for detecting the genotype of the PDGF-B gene rs1800818 site

[0037] 1. Genomic DNA extraction

[0038] Extract the genomic DNA of the sample to be tested and use it as a template for genotype detection.

[0039] 2. Design and synthesis of primers

[0040] Use the Genotyping Tools and MassARRAY Assay Design software of Sequenom Company to design PCR amplification primers and single-base extension primers for the SNP sites to be tested, and submit them to biological companies for synthesis. The sequence of the specific primer pair for detecting the PDGF-B gene rs1800818 site genotype is: forward primer 5'-ACGTTGGATGCCGCAGAGGACGCCCAGAG-3' (SEQ ID No.1 in the sequence listing); reverse primer 5'-ACGTTGGATGACAGGTGGACGCGGCGCGA-3 ' (SEQ ID No.2 in the Sequence Listing). The extended primer sequence used for the single base amplification reaction is 5'-ccCGGTCCGTCTGCCCGCC-3' (SEQ ID No.3 in the sequence listing).

[0041] 3. Establish...

Embodiment 2

[0064] Example 2 Analysis of the rs1800818 site of PDGF-B gene and the susceptibility of fever with thrombocytopenia syndrome caused by Novel Bunia virus

[0065] Using the method established in Example 1, the genotype of the rs1800818 locus of the PDGF-B gene in the Chinese population (430 patients with fever and thrombocytopenia syndrome caused by New Bunia virus and 633 controls) was analyzed.

[0066] 1. Research object

[0067] Case group: 430 patients with fever and thrombocytopenia syndrome caused by new bunya virus, all of whom met the clinical diagnostic criteria for patients with fever and thrombocytopenia syndrome caused by new bunya virus and were confirmed by nucleic acid detection of new bunya virus .

[0068] Control group: 633 patients with latent neobunia virus infection who were negative for neobunia virus IgM antibody but positive for neobunia virus IgG antibody.

[0069] All subjects were unrelated Chinese Han people. All subjects signed an informed cons...

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Abstract

The invention discloses application of rs1800818 to detection of severe fever with thrombocytopenia syndrome (FTLS) caused by severe fever with thrombocytopenia syndrome virus (FTLSV). The technical scheme to be protected is about application of substances for detecting the polymorphism or the genotype of rs1800818 in a personal genome to preparation of a product for screening FTLS and application of substances for detecting the polymorphism or the genotype of rs1800818 in a personal genome to preparation of a product for forecasting the condition of an FTLS patient. The substances for detecting the polymorphism or the genotype of rs1800818 can be combined with other substances (such as a substance for detecting other single nucleotide polymorphism or genotype related to FTLS) to prepare a product for screening FTLS patients or forecasting the conditions of FTLS patients.

Description

technical field [0001] The invention relates to the application of rs1800818 in the detection of neobunia virus-induced fever with thrombocytopenia syndrome in the field of biomedicine. Background technique [0002] Fever with thrombocytopenia syndrome (Severe fever with thrombocytopenia syndrome, SFTS), also known as fever, thrombocytopenia and leukopenia syndrome (Fever, Thrombocytopenia and Leukopenia Syndrome, FTLS), is a new type of Bunia syndrome that appeared in my country. Virus (Severe fever with thrombocytopenia syndrome bunya virus, SFTSV, referred to as new bunya virus) is a new infectious disease that is infected through the blood. The main clinical manifestation is acute fever (mostly above 38°C) ), thrombocytopenia and leukopenia, bleeding gums, skin ecchymosis and other bleeding symptoms in some cases, and a small number of patients may die due to multiple organ damage. There is no effective vaccine or treatment for the disease. Studies have shown that SFTSV ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 刘玮张小爱曹务春
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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