Array digital PCR chip and application method thereof

Abstract

The invention specifically relates to an array digital PCR chip and an application method thereof, belonging to the technical field of experimental instruments used in biochemistry. The array digital PCR chip comprises a substrate and a cover plate, wherein the upper surface of the substrate is provided with a plurality of chutes; the central part of the bottom surface each chute is recessed to form a PCR reaction cell; and the lower surface of the cover plate is provided with a plurality of slide block bulges respectively in snapping connection with the chutes. When the cover plate covers the substrate, the slide block bulges are snapped with the corresponding chutes; and a liquid storage chamber is formed between each slide block bulge and its corresponding chute. According to the invention, through cooperation between the substrate and the cover plate, liquid in each liquid storage chamber simultaneously enters the PCR reaction cells and is mixed with primers and probes in the PCR reaction cells when the cover plate slides, so non-specific reaction is avoided, and the accuracy of subsequent detection data is improved.

Description

technical field [0001] The invention relates to a test instrument in the field of biochemistry, in particular to an array digital PCR chip and a method for using the same. Background technique [0002] Polymerase chain reaction (PCR) has been widely used in medicine, genetics, microbiology and even the entire life sciences. Real-time quantitative PCR array technology is based on real-time quantitative PCR technology as the core, combined with array technology, a new amplification technology that can detect a series of batch genes in a single experiment. This technology can reliably and accurately detect gene expression profiles related to important biological processes such as signal transduction and disease-related pathways on a large scale, and has important applications in disciplines such as microorganisms, genetic diseases, tumors, and pharmacogenomics. [0003] In the prior art, a digital polymerase chain reaction (Digital PCR) is performed using a substrate design ch...

AI Technical Summary

Problems solved by technology

Two glass slides with a channel structure are overlapped and loaded, so that the channels on both sides are merged into a complete flow channel, and there are N multiple reaction pools in the substrate, and the flow channel will add the liquid to be tested to each reaction pool in turn. In this time difference, part of the reaction pools will obtain the liquid to be tested in advance, which will be mixed with the primers and probes in the reaction pools, resulting in non-specific reactions, and non-specific PCR products will interfere with the detection results; and the reaction at the front of the flow channel The primer and probe substances in the pool are easily brought into the next reaction pool in a small amount due to the force of liquid flow. Multiple primers and probes are mixed, and multiple products are produced in the same reaction pool, resulting in uninterpretable results.

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