The invention discloses a cultivation method of Morchella esculenta, and the cultivation method includes the following steps: S1. preparing a Morchella esculenta strain: the Morchella esculenta strainis composed of a type A strain and a type B strain, wherein the type A strain includes the following raw materials in percentages by weight (a total of 100%): 65-75% of wheat grains, 20-30% of rice husks, 1-5% of gypsum, 0.2-1% of K2(subscript)PO4(subscript) and 0.2-1% of MgSO4(subscript); and the type B strain includes the following raw materials in percentages by weight (a total of 100%): 82-88% of cotton seed hulls, 5-10% of three powder, 0.5-2% of brown sugar, 0.5-2% of gypsum, 1.5-4% of quicklime, 0.2-1% of K2(subscript)PO4(subscript) and 0.2-1% of MgSO4(subscript); S2, sowing the type Astrain, spraying special strain nutrient solution for the Morchella esculenta, and carrying out soil covering; S3, sowing the type B strain on a ridge surface after soil covering, carrying out mulchfilm covering, and then carrying out a nutrition bag treatment; and S4, carrying out mushroom inducing and cultivating treatments, thereby obtaining the Morchella esculenta. The cultivation method ofthe Morchella esculenta provided by the invention solves technical problems of high and stable yield of artificial cultivation of the Morchella esculenta by optimizing the composition formula and thesowing mode of the Morchella esculenta, and makes the nutritional value and the flavor of the cultivated Morchella esculenta similar to wild Morchella esculenta at the same time.