Primer and test kit for efficiently detecting porcine Japanese encephalitis virus

Abstract

The invention discloses a primer and a test kit for efficiently detecting porcine Japanese encephalitis virus; an improved LAMP technology is taken as a gene amplification reaction principle, gold nanoparticles are added into a reaction system, and ssDNA and protease are adsorbed, thereby inhibiting non-specific reaction in a heating process, achieving the purpose of hot start, and avoiding non-specific reaction in the heating process; the improved LAMP technology and a microfluidic chip technology are combined, an accurate detection result can be rapidly given, the purpose of joint inspectionof multiple different indexes of the same sample is achieved, and meanwhile, a reaction reagent is pre-buried in a microfluidic chip, a user only needs to add the sample, and operation is easy and convenient.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a primer and a kit for efficiently detecting porcine epidemic Japanese encephalitis virus. Background technique [0002] Porcine encephalitis Japanese encephalitis is caused by Japanese encephalitis virus. It is mainly characterized by sow abortion, stillbirth and boar orchitis. Epidemic encephalitis virus is also known as Japanese encephalitis virus (JEV), referred to as JE virus, and the disease caused by it is referred to as JE. JE is a zoonotic viral infectious disease that can infect Humans and various animals can cause central nervous system diseases in humans and reproductive disorders in pigs, seriously endangering human health and the development of animal husbandry. The current diagnostic methods are: immunocytochemical method and RT-PCR technology. Using the existing PCR technology, because the reaction needs to cycle in two different temperature ...

AI Technical Summary

Problems solved by technology

Using the existing PCR technology, because the reaction needs to be cycled in two different temperature zones, the requirements for the instrument are high, and the cost is relatively high, and the PCR technology only has one pair of amplification primers, which is susceptible to interference and relatively insufficient in specificity, and It takes a long time to produce results, high professional requirements for operation, and many micro-dosing steps

The LAMP technology has 4 different specific primers, so the accuracy of the detection results is higher, but because it is a constant temperature reaction, it lacks a hot-start enzyme similar to PCR, and it is easy to produce non-specific amplification during the heating stage of the equipment, which will affect the detection results.

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