Technology for improving activity of enzyme-labeled working fluid anti-human IgA alkaline phosphatase

A technology of phosphatase activity and process method, which is applied in the field of improving the anti-human IgA alkaline phosphatase activity of an enzyme-labeled working solution, can solve the problems of long enzyme reaction time, unfavorable promotion, missed detection, etc., so as to improve the reaction activity and avoid non-specific Heterosexual reaction, the effect of improving the binding speed

Inactive Publication Date: 2015-02-11
BEIJING H&J NOVOMED
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Problems solved by technology

Such as affinity, pH, etc. Select the corresponding enzyme-labeled secondary antibody working solution, which is easily interfered by the external experimental environment in many tests, resulting in missed detection. In some tests, the reaction time of the enzyme is long, which is not conducive to clinical promotion.

Method used

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  • Technology for improving activity of enzyme-labeled working fluid anti-human IgA alkaline phosphatase

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Experimental program
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Embodiment

[0021] A. Prepare enzyme-labeled secondary antibody working solution, the enzyme-labeled secondary antibody working solution is phosphate buffer containing protein;

[0022] B. Add polyethylene glycol 6000 with a concentration of 0.5% by weight to the above-mentioned enzyme-labeled secondary antibody working solution, and stir to fully dissolve it;

[0023] C. Then add anti-human IgA alkaline phosphatase with a weight percentage concentration of 0.06 / % to the enzyme-labeled secondary antibody working solution adding polyethylene glycol 6000, stir, and make it fully dissolved; obtain improved anti-human IgA alkaline phosphatase Enzyme-active enzyme-labeled secondary antibody working solution.

[0024] like figure 1 As shown, Nos. 1-5 are EA-IgA positive samples, and Nos. 6-8 are EA-IgA negative samples.

[0025] Row A is the detection result using the method of the present invention, and Row B is the detection result using the prior art. There is no color band in the middle ...

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Abstract

The invention provides a technology for improving activity of an enzyme-labeled working fluid anti-human IgA alkaline phosphatase. The technology comprises preparing an enzyme-labeled secondary antibody working solution according to the characteristics of anti-human IgA alkaline phosphatase self. The improvement comprises successively adding the following substances into the prepared enzyme-labeled secondary antibody: (1) adding a low-concentration high-molecular polymer, and dissolving the added high-molecular polymer in the prepared enzyme-labeled secondary antibody working solution; and (2) then adding anti-human IgA alkaline phosphatase into the enzyme-labeled secondary antibody working solution added with the high-molecular polymer, and stirring to fully dissolve the anti-human IgA alkaline phosphatase, so as to obtain an enzyme-labeled secondary antibody working solution with activity-improved anti-human IgA alkaline phosphatase. According the method, by adding the low-concentration high-molecular polymer, the effects of improving the reaction activity of anti-human IgA alkaline phosphatase and improving the detection sensitivity are realized.

Description

technical field [0001] The invention relates to the preparation of biological in vitro diagnostic reagents, in particular to a process method for improving the anti-human IgA alkaline phosphatase activity of an enzyme-labeled working solution. Background technique [0002] At present, immunological detection technology and its products have been widely used in many detection fields such as major infectious diseases, autoimmune diseases, tumor markers, food safety and prenatal and postnatal care. In the immunospot test and colloidal gold rapid detection test, enzyme is an essential and important part of the test. At present, the application of enzymes is based on the characteristics of the enzyme itself. Selecting the corresponding enzyme-labeled secondary antibody working solution, such as affinity, pH, etc., is easily interfered by the external experimental environment in many tests, resulting in missed detection. In some tests, the reaction time of the enzyme is long, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/558
CPCG01N33/535
Inventor 朱京山
Owner BEIJING H&J NOVOMED
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