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Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent

A pesticide residue detection, microfluidic chip technology, applied in analytical materials, instruments, etc., can solve the problems of increasing the operating intensity of analysts, inconvenient solution carrying, and lengthy configuration process, avoiding solution preparation work, and operating concise and important. The effect of applying value

Inactive Publication Date: 2015-07-22
HANGZHOU TINKER BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the on-site detection of pesticide residues, it is still necessary to carry or configure various reaction reagent solutions such as extraction solution, enzyme solution, chromogenic reagent solution, and substrate solution, and then manually transfer various solutions to the microfluidic chip to increase the analysis time. The operation intensity of personnel, and the solution is inconvenient to carry; the configuration process is lengthy, which can no longer meet the needs of rapid detection of pesticide residues, and has become a bottleneck in the application of microfluidic analysis technology in the field of rapid detection of agricultural products

Method used

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  • Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent
  • Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent
  • Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent

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Embodiment Construction

[0016] specific implementation plan

[0017] The following examples will further illustrate the present invention, but do not limit the present invention thereby.

[0018] Such as figure 1 As shown, the microfluidic chip for detecting pesticide residues with pre-stored reaction reagents provided by the present invention includes a liquid sac pool, a liquid sac, a sample pool, a microfluidic channel, two reagent pools and reagents.

[0019] During the detection, it is only necessary to put a quantitative sample to be tested into the chip sample pool 3, close the sample chamber sealing cover 10, release the extraction liquid 6 in the liquid bag, and the vent hole 9 is connected to an external pressure regulating valve to make the internal chamber of the chip and the air generated. The pressure difference drives the flow of the liquid, the extract enters the sample pool 3 and is mixed with the sample to be tested for extraction, then the mixed liquid enters the reagent pool 4, f...

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Abstract

The invention provides a pesticide residue detection micro-fluidic chip with a pre-stored reaction reagent. The pesticide residue detection micro-fluidic chip is characterized by comprising a micro-fluidic channel, a liquid sac pool, an extracting solution sac, a sample pool, a sample pool cover, a plurality of reagent pools and bio-reagents stored in the reagent pools. During detection, a to-be-detected sample just needs to be put into the sample pool of the chip, a reagent solution such as an extracting solution, an enzyme liquid, a color developing agent solution or a primer solution does not need to be prepared. The chip is concise to operate and suitable for field detection of pesticide residues and has an extremely important application value.

Description

technical field [0001] The invention relates to the field of microfluidic chip analysis, in particular to a microfluidic chip used for on-site detection of pesticide residues. Background technique [0002] At present, microfluidic chip analysis technology has been applied to the rapid detection of organic pesticide residues due to its significant advantages such as miniaturization, integration, high throughput, and low cost. However, in the on-site detection of pesticide residues, it is still necessary to carry or configure various reaction reagent solutions such as extraction solution, enzyme solution, chromogenic reagent solution, and substrate solution, and then manually transfer various solutions to the microfluidic chip to increase the analysis time. The operation intensity of personnel, and the solution is inconvenient to carry; the configuration process is lengthy, which can no longer meet the needs of rapid detection of pesticide residues, and has become a bottleneck...

Claims

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Application Information

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IPC IPC(8): G01N35/00
Inventor 叶嘉明王晓东杨平范真真周海梦
Owner HANGZHOU TINKER BIOTECHNOLOGY CO LTD
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