Combined device for rapidly and quantitatively detecting multiple tumor markers

A tumor marker and detection device technology, which is applied in the field of rapid quantitative combined detection devices for multiple tumor markers, can solve problems such as low positive rate, and achieve the effects of simple operation, rapid response and accurate results

Inactive Publication Date: 2014-01-22
天津中新科炬生物制药股份有限公司
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Problems solved by technology

[0025] 3) Other non-malignant tumors also have different degrees of increase, but the positive rate is low: such as endometriosis, pelvic inflammatory disease, ovarian cyst, pancreatitis, hepatitis, liver cirrhosis, etc.
At present, there is no reagent that uses the colloidal gold method to quantitatively detect multiple indicators.

Method used

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  • Combined device for rapidly and quantitatively detecting multiple tumor markers
  • Combined device for rapidly and quantitatively detecting multiple tumor markers

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Embodiment

[0070] Example: Preparation of AFP / CEA / PSA Three Tumor Markers Rapid Quantitative Combined Detection Device

[0071] 1 main material

[0072] 1.1 AFP, CEA and PSA paired antibodies from biological raw materials were purchased from related companies; mouse anti-human IgG antibody, goat anti-mouse IgG: self-made; chloroauric acid: product of Sigma Company; NC membrane: product of Millipore Company of the United States; hydrolyzed casein, poly Ethylene glycol PEG20000: a product of Sigma. Other commonly used reagents are analytical reagents.

[0073] 1.2 The company obtained 120 clinical samples in related hospitals, including 60 serum samples from patients diagnosed with various tumors. The detection value of AFP was from 200-1000ng / mL, and the detection value of CEA was from 20-200ng / mL. PSA detection value from 10-60ng / mL, 60 normal human serum samples, the detection value is lower than the corresponding reference value.

[0074] 1.3 Triple clamping shell: designed by our c...

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Abstract

A combined device for rapidly and quantitatively detecting multiple tumor markers is characterized by being composed of 3 to 8 pieces of independent tumor marker test paper such as AFP, CEA, PSA, CA125, CA153, CA199, CA242 and NSE, a clamping shell special for the 3 to 8 pieces of test paper, and an immune layer result analysis and judgment recorder with built-in corresponding standard curves and the judgment method. The test paper can be combined to form the detection device with the 3 to 8 pieces of test paper according to the specific requirements. The standard curves and the judgment method for the 8 pieces of test paper are built in the immune layer result analysis and judgment recorder and used for conducting quantitative judgment on test results, and forecasting of various tumor occurrence conditions can be conducted on different types of combination of the test results through the judgment method. Detection is completed within 15 minutes to 20 minutes. The combined device for rapidly and quantitatively detecting the multiple tumor markers has the advantages of being combined in detection, easy and convenient to operate, rapid in response, accurate in result, suitable for on-site test and the like, and the requirements for more accurate, easier and move convenient clinical detection are met.

Description

technical field [0001] The patent of the present invention relates to the technical field of medical clinical diagnosis, in particular to a rapid quantitative combined detection device for various tumor markers prepared by an immunocolloidal gold rapid detection method. Background technique [0002] Certain substances produced and released by tumor cells often exist in tumor cells or in host body fluids in the form of metabolites such as antigens, enzymes, and hormones. Tumors can be identified or diagnosed based on their biochemical or immune characteristics. Tumor markers are mainly used clinically for the discovery of primary tumors, the screening of high-risk groups for tumors, the differential diagnosis of benign and malignant tumors, the judgment of tumor development, the observation and evaluation of tumor treatment effects, and the prediction of tumor recurrence and prognosis, etc. . [0003] Common tumor markers are: [0004] 1. Alpha-fetoprotein [0005] 1) Prim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484G01N33/558G01N33/57434G01N33/57473
Inventor 李洲杨发青许俊艳
Owner 天津中新科炬生物制药股份有限公司
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