Immuno magnetic bead and producing method, and method and test plate for detection

Abstract

An immune bead is made up of the magnetic carrier micro ball which combines at least one immune matching base. The micro ball is composed of the magnetic nm particle and the high molecular framework material which the core is the metal particle; the out of the core is high molecular framework, the out layer is the functional layer which can combines functional gene of different immune matching base. The manufacture method includes: the bead pretreatment, the bead activation, manufacture of the coupling antibody, closing the antibody with the confining liquid and purifying the immune bead. The detecting method is to detect the different things by the immunological response sandwich, the competition and the indirect method and set the control system on the testing board. The board is made up of the encrusting test paper, the coupling mat, the sample mat, the water suction mat, the coving film and the testing board outside calipers. It has the high sensitivity and accurate quantity; the regent is simple and cost low.

Description

technical field [0001] The present invention relates to a marker for immunological detection, a method for making the marker, a method for detection and a test plate, in particular to an immunomagnetic bead, a method for making an immunomagnetic bead, and a method for detection and test board. Background technique [0002] Immunoassay is a method of analyzing and determining the corresponding antigen or antibody to be tested by using the property of antibody (Antibody) to specifically bind to corresponding antigen (Antigen) and hapten, by using specific antibody or antigen as a selective reagent. With the interpenetration of disciplines, the scope of immunology and its application continues to expand, and new immunological detection methods continue to emerge, playing an increasingly important role in the research and application of biology, medicine, agronomy and food science. role. In order to improve the sensitivity of antigen and antibody detection, the substances that...

AI Technical Summary

Problems solved by technology

Radioimmunity: high sensitivity, but the operator needs to be exposed to radioactive substances, which will cause environmental pollution after the measurement is completed; Fluorescence immunity: low sensitivity, expensive instruments, vulnerable to scattered light, background fluorescence from samples, and fluorescence quenching during analysis The interference of factors such as extinction; the sensitivity of chemiluminescence immunoassay is good, but the equipment is expensive, and the colloidal gold immune reaction is fast, but the sensitivity and specificity are poor; the most widely used at present is enzyme-linked immunoassay, its basic characteristics are in the middle of the above items, and the sensitivity Lower than radioimmunity, but the reaction time is long, the reaction is highly dependent on temperature, and cannot be quantified

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