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Identification method of ganoderma lucidum spore oil

A technology of Ganoderma lucidum spore oil and soybean oil, which is applied in the field of analytical chemistry to achieve the effect of being suitable for on-site detection and broad application prospects

Inactive Publication Date: 2016-06-01
惠州市食品药品检验所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method for identifying Ganoderma lucidum spore oil, which can simply, quickly and effectively distinguish Ganoderma lucidum spore oil from rapeseed oil, soybean oil, corn oil, sunflower oil, peanut oil, and olive oil, aiming to solve the problem in the market. The lack of rapid identification method of Ganoderma lucidum spore oil

Method used

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  • Identification method of ganoderma lucidum spore oil
  • Identification method of ganoderma lucidum spore oil
  • Identification method of ganoderma lucidum spore oil

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Embodiment 1

[0047] The Ganoderma lucidum spore oil (100%) soft capsules of 9 brands were purchased from the market, and were detected by Raman spectroscopy as follows: the instrument used for spectral scanning was laser Raman spectrometer RamTracer-200, OptoTrace software. Conditions for spectral scanning: the laser wavelength is 785nm, and the laser power is 200mW. The scanning range of spectral scanning is 250cm -1 ~2300cm -1 , the integration time is 10s. Signal peak area = △ displacement value × peak intensity / 2.

[0048] Put the empty sample bottle into the detection cell as a blank control to subtract the background. Draw 1mL of sample into the sample bottle and place it in the sample chamber of the laser Raman spectrometer for detection. The specific process is:

[0049] The sample to be tested is scanned by laser spectrum to obtain its characteristic Raman spectrum; use the OptoTrace analysis software to perform baseline correction on the obtained characteristic Raman spectru...

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Abstract

The invention is applicable to the field of analytical chemistry and in particular relates to an identification method of ganoderma lucidum spore oil. The method is used for distinguishing the ganoderma lucidum spore oil from rapeseed oil, soybean oil, corn oil, sunflower seed oil, peanut oil and olive oil, and comprises the following steps: carrying out laser spectrum scanning on a sample to be detected to obtain a characteristic Raman spectrogram of the sample; comparing the Raman spectrogram with standard conditions, wherein the standard conditions are as follows: a displacement value at a first part is 1300+ / -3cm<1> and a displacement value at a second part is 1262+ / -3cm<1>, the Raman spectrum intensity values of the first part and the second part are more than 200, and the ratio of the Raman spectrum intensity values of the first part and the second part, and the ratio of signal peak areas of the first part and the second part are more than 2; and determining the content of ergosterol on a sample meeting the standards, wherein the sample with the content of the ergosterol of 0.10-2.79mg / g is the ganoderma lucidum spore oil. The identification method provided by the invention is simple and rapid, and has a wide application prospect in the aspect of rapid identification of the ganoderma lucidum spore oil and other common vegetable oil.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for identifying ganoderma lucidum spore oil. Background technique [0002] Ganoderma lucidum spore oil is oily substance extracted from Ganoderma lucidum spore powder, which accounts for about 20% to 30% of the mass of Ganoderma lucidum spores, and concentrates many active ingredients in Ganoderma lucidum spores. Ganoderma lucidum spore oil mainly contains triterpenoid ganoderma acid, unsaturated fatty acids, sterols, vitamins and other functional ingredients, which have the functions of inhibiting tumor cell growth, anti-cancer, immunity, liver protection, hypoglycemia, and anti-oxidation. Just because Ganoderma lucidum spore oil has these special physiological effects, its products are expensive. Ganoderma lucidum spore oil and its related products sold in the market are of various varieties with uneven quality. Fatty acid is one of the main components of Ganoderm...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N30/02
CPCG01N21/65G01N30/02G01N2030/027
Inventor 黄秀丽詹云丽王晓琴赖政炀陈文财宁焕焱曾宪远
Owner 惠州市食品药品检验所
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