Method for preparing nanogold through mango peel reducing agent
A mango peel and reducing agent technology, applied in the field of biotechnology and nano research, can solve the problems of environmental and biological hazards, large toxic and side effects, and environmental pollution, and achieve the effect of not easy to reunite, simple steps, and good dispersion
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Embodiment 1
[0027] Nano gold is prepared by the method of the invention.
[0028] (1) Preparation of chloroauric acid solution First weigh 0.041g of chloroauric acid powder and dissolve it in 100mL of distilled water to obtain 1mmol of chloroauric acid solution. Since chloroauric acid is highly corrosive, metal medicines should not be used during operation. spoon, then adjust the pH value of the chloroauric acid solution to 9.0 with aqueous sodium hydroxide solution;
[0029] (2) Preparation of mango peel reducing agent Take fresh mango peel and wash it with distilled water for 3 times, dry it in an oven at 80°C, then crush it to 100 mesh, put it in a ziplock bag for later use;
[0030] (3) Preparation of gold nanoparticles Measure 50mL of the chloroauric acid solution prepared in (1), add 3g of the mango peel reducing agent prepared in (2), stir well, and bathe in water for 100min at 25°C, observe the color of the solution. When the acid solution changes from yellow to purple, the react...
Embodiment 2
[0033] Nano gold is prepared by the method of the invention.
[0034] (1) Preparation of chloroauric acid solution First weigh 0.041g of chloroauric acid powder and dissolve it in 100mL of distilled water to obtain 1mmol of chloroauric acid solution, then adjust the pH value of the chloroauric acid solution to 7.0 with sodium hydroxide aqueous solution;
[0035] (2) Preparation of mango peel reducing agent Take fresh mango peel and wash it twice with distilled water, freeze-dry it under vacuum at -80°C, take the freeze-dried powder and grind it through a 100-mesh sieve, put it in a ziplock bag for later use;
[0036] (3) Preparation of gold nanoparticles Measure 50 mL of the chloroauric acid solution prepared in (1), add 1 g of the mango peel reducing agent prepared in (2), stir well, and bathe in water at 30°C for 90 minutes, observe the color of the solution, when the gold chloride When the acid solution changes from yellow to purple, the reaction is terminated; the purple-r...
Embodiment 3
[0039] Nano gold is prepared by the method of the invention.
[0040](1) Preparation of chloroauric acid solution First weigh 0.041g of chloroauric acid powder and dissolve it in 100mL of distilled water to obtain 1mmol of chloroauric acid solution, then adjust the pH value of the chloroauric acid solution to 8.0 with sodium hydroxide aqueous solution;
[0041] (2) Preparation of mango peel reducing agent Wash fresh mango peel with distilled water for 3 times, dry naturally, crush and pass through a 100-mesh sieve, then mix fresh mango peel: ethanol: water volume ratio of 1:1.5:1.5 and reflux Extract for 3 hours, and store the extract in a 4°C refrigerator for later use;
[0042] (3) Preparation of gold nanoparticles Measure 50 mL of the chloroauric acid solution prepared in (1), add 10 mL of mango peel reducing agent extract prepared in (2), stir well, put in a water bath at 28°C for 95 minutes, and observe the color of the solution , when the chloroauric acid solution chang...
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