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Human BNP immunospecific antibodies

Inactive Publication Date: 2007-09-06
ABBOTT LAB INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014] In one aspect, the present invention relates to an isolated antibody which immunospecifically binds to an epitope comprising amino acid residues 5 through 13 of human brain natriuretic peptide (“hBNP”) with at least about a two fold improvement in its equilibrium dissociation constant (KD) when compared with an antibody produced by hybridoma cell line 106.3, said cell line having A.T.C.C. Accession No. HB-12044. More specifically, the antibody of the present invention exhibits at least about a three fold improvement, at least about a five fold improvement, at least about a ten fold improvement, at least about a fifteen fold im

Problems solved by technology

One of the problems with natural human natriuretic peptides is that they are unstable in plasma and serum.

Method used

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Examples

Experimental program
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Effect test

example 1

Identification of Immunoglobulin Genes

[0307] Messenger RNA was isolated from subcloned anti-BNP 106.3 hybridoma cells (hybridoma cell line 106.3 (A.T.C.C. Accession No. HB-12044) is described in U.S. Pat. No. 6,162,902). 106.3 mRNA was utilized in a reverse transcriptase-polymerase chain reaction using a mouse Ig primer set kit purchased from Novagen (Novagen (which is an Affiliate of Merck KGaA, Darmstadt, Germany), Cat No. 69831-3) with immunoglobulin gene specific primers contained in the kit. The resulting PCR products were sequenced and thus the immunoglobulin variable heavy and variable light chain genes were identified (See FIGS. 3A-3E and SEQ ID NO:1).

Cloning 106.3 Variable Region Genes into pYD41 Vector

[0308] A yeast display system was used to express unmutated anti-BNP proteins (described herein infra) and a library of anti-BNP proteins on the yeast surface as a fusion to the yeast protein AGA2. A yeast display vector called pYD (Invitrogen, Carlsbad, Calif.), was use...

example 2

ATCC Deposit Information

[0339] Chinese Hamster Ovary cell line for BNP106.3sc128am1CHO1162-236 was deposited with the American Type Culture Collection (hereinafter referred to as “A.T.C.C.”), 10801 University Blvd., Manassas, Va. 20110-2209, on Sep. 20, 2005 and assigned A.T.C.C. Accession No. PTA-6987.

example 3

Competitive Immunoassay Using a Single Antibody Format

[0340] The antibody produced by CHO cell line AM1 (“antibody AM1”) described above in Examples 1 and 2 was purified and tested to determine the antibody's ability to bind human cyclic BNP1-32 in a single antibody format on the ARCHITECT® instrument (Abbott Laboratories, Abbott Park, Ill. This instrument is described in U.S. Pat. No. 5,468,646). This single antibody format encompasses the use of only one analyte specific antibody in the testing reaction.

[0341] Paramagnetic microparticles (hereinafter “microparticles”, Polymer Labs, Amherst, Mass.) were washed and then reacted with serially diluted Goat anti-human antibody (Jackson ImmunoResearch, West Grove, Pa.). The Goat anti-human antibody was coated onto the paramagnetic microparticles using the techniques described in U.S. Pat. No. 6,162,902. Specifically, EDAC coupling was used (EDAC is generally used as a carboxyl activating agent for amide bonding with primary amines. In...

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Abstract

The present invention relates to antibodies that immunospecifically bind to human brain natriuretic peptide or a human brain natriuretic peptide fragment with a high binding affinity, methods for producing and selecting said antibodies, immunoassays for human brain natriuretic peptide or a human brain natriuretic peptide fragment that employ said antibodies and therapeutic compositions containing said antibodies.

Description

RELATED APPLICATION INFORMATION [0001] This application claims the benefit of co-pending U.S. Application No. 60 / 734,964, filed Nov. 9, 2005, which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION [0002] The present invention relates to antibodies that immunospecifically bind to human brain natriuretic peptide or a human brain natriuretic peptide fragment with a high binding affinity, methods for producing and selecting said antibodies, immunoassays for human brain natriuretic peptide or a human brain natriuretic peptide fragment that employ said antibodies and therapeutic compositions containing said antibodies. BACKGROUND OF THE INVENTION [0003] Atrial natriuretic peptide (hereinafter referred to as “ANP”), brain natriuretic peptide (hereinafter referred to as “BNP”), C-type natriuretic peptide (hereinafter referred to as “CNP”) and Dendroaspis natriuretic peptide (hereinafter referred to as “DNP”) are each members of a family of hormones kliown as “natri...

Claims

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Application Information

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IPC IPC(8): A61K39/395C07K16/28G01N33/567
CPCA61K2039/505C07K16/26C07K2317/24C07K2317/622Y10S435/975G01N33/74G01N2333/58G01N2800/325C07K2317/92A61P13/12A61P9/00A61P9/04A61P9/06A61P9/12
Inventor BROPHY, SUSAN E.TYNER, JOAN D.TU, BAILIN X.PINKUS, MARY S.SHIH, JESSIE W.
Owner ABBOTT LAB INC
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