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Cardiac troponin I/N-terminal pro-brain natriuretic peptide/D-dimer three-in-one detection kit and preparation method

A technology of cardiac troponin and natriuretic peptide, which is applied in the field of three-in-one assay kits, can solve the problems of strong interference and high intensity, and achieve the effects of high sensitivity, rapid response, and clear clinical guidance

Inactive Publication Date: 2017-06-13
WEIHAI NEOPROBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the usual fluorescence measurement, because the test sample contains a variety of fluorescent components, the background fluorescence (scattered light caused by colloidal particles and solvent molecules in the sample and non-specific fluorescence emitted by proteins and other compounds in the serum) has a large intensity and interference. Strong, become the bottleneck of large-scale promotion of fluorescence analysis

Method used

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  • Cardiac troponin I/N-terminal pro-brain natriuretic peptide/D-dimer three-in-one detection kit and preparation method
  • Cardiac troponin I/N-terminal pro-brain natriuretic peptide/D-dimer three-in-one detection kit and preparation method
  • Cardiac troponin I/N-terminal pro-brain natriuretic peptide/D-dimer three-in-one detection kit and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The various components of the test paper card in the cardiac troponin I / N-telencephalin natriuretic peptide precursor / D dimer three-in-one assay kit can be prepared by the following measures:

[0047] 1. Preparation of sample pad 2:

[0048] Soak the glass fiber membrane in the treatment solution containing 1.0% Triton X-100, 2.5% BSA, 0.15M Tris buffer, pH7.5, soak at 4°C for 4 hours, then place it in an oven and dry it at 37°C 2 hours. 2. Preparation of binding pad 3 for absorbing fluorescent microsphere-labeled antibody:

[0049] Soak the glass fiber membrane in 150mM Tris-HCL treatment solution (containing 1.0% Triton X-100, 2.5% BSA, pH7.4), soak at 4°C for 2 hours, then take it out of a 37°C oven and dry it for 4 hours, and set it aside. The glass fiber membrane was placed on the Bio-DotXYZ3050 three-dimensional spraying platform, and the rare earth fluorescent microspheres labeled cardiac troponin I, N-terminal natriuretic peptide precursor and D2 were injected...

Embodiment 2

[0058] Embodiment 2: accuracy test

[0059] Select the above-mentioned test paper card and fluorescent immunochromatography analyzer (model: NEO-007), the setting of the parameters of the fluorescent immune analyzer: after setting the process parameters of the test paper card on the fluorescent immune analyzer, take the above-mentioned assembled test paper card , with 0.2, 1, 5, 10, 20, 30, 40ng / mL of cTnI, 100, 1000, 2000, 5000, 10000, 15000, 20000pg / mL of NT-proBNP, 0.1, 0.2, 0.5, 1, 2 , 5. 10ug / mL D-Dimer calibrator was measured with a test paper card to obtain the fluorescence intensity value of each calibrator, and the results were input into the parameters of the analyzer to complete the setting of the parameters of the analyzer.

[0060] Main testing materials: clinical samples were obtained from relevant hospitals, a total of 200 latex-enhanced immunoturbidimetric value samples, including 100 plasma samples, 100 whole blood samples, cardiac troponin I / N-telencephalin n...

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Abstract

The invention relates to the technical field of fluorescence immunochromatography in medical immunology and in particular relates to a cardiac troponin I / N-terminal pro-brain natriuretic peptide / D-dimer three-in-one detection kit and a preparation method. The cardiac troponin I / N-terminal pro-brain natriuretic peptide / D-dimer three-in-one detection kit is provided with a test paper card and is characterized in that the test paper card is provided with a PVC (Polyvinyl Chloride) plate, a sample pad, a conjugate pad, a nitrocellulose membrane and a water absorption pad from top to bottom, wherein the conjugate pad is adsorbed with three monoclonal antibody-microsphere coupled compounds, and three monoclonal antibodies are labeled with rare-earth Eu<3+> fluorescent microspheres respectively and comprise anti-cardiac troponin I, anti-N-terminal pro-brain natriuretic peptide and anti-D-dimer; the diameter of the rare-earth fluorescent microspheres is 150nm and the rare-earth fluorescent microspheres contain a rare-earth lanthanide element Eu<3+>, are stable under a basic state and emit fluorescent light with the wavelength of 615nm under the action of an excitation light source with the wavelength of 337nm; the monoclonal antibodies are monoclonal antibodies obtained by purifying and mixing and are selected from 2 to 6 different monoclonal antibody cell strains which are epitope-tagged with different antigens including cardiac troponin I, N-terminal pro-brain natriuretic peptide and D-dimer respectively. The cardiac troponin I / N-terminal pro-brain natriuretic peptide / D-dimer three-in-one detection kit has the advantages of simplicity and convenience for operation, rapid reaction, high sensitivity, high specificity and the like.

Description

[0001] Technical field: [0002] The invention relates to the technical field of fluorescent immunochromatography in medical immunology, in particular to a rapid and accurate detection of cardiac troponin I, N-terminal natriuretic peptide precursor, and D2 in plasma and whole blood samples simultaneously. A three-in-one assay kit and preparation method for quantitative analysis of three cardiovascular and cerebrovascular disease-related indicators such as polymers. [0003] Background technique: [0004] Cardiac Troponin I (cTnI) can be used as a marker of myocardial injury. Due to its high myocardial specificity, high sensitivity to myocardial injury and long window period, it has not only become the "gold standard" for judging myocardial injury, especially acute myocardial infarction. And it has become the most suitable marker to judge the risk of myocardial injury in patients with coronary syndrome. Elevation of troponin can also be used as strong evidence to support clini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/533G01N33/543
CPCG01N33/533G01N33/54313G01N33/582G01N33/6887G01N33/6893G01N2800/325Y02A50/30
Inventor 滕志涛孙宁宁于鸿翔刘衍亮宋璐琳陈萍萍
Owner WEIHAI NEOPROBIO
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