This invention discloses a liquid-phase probe-targeted capture
library construction method and kit for enteroviruses, belonging to the field of
virus detection technology. This invention combines targeted enrichment with high-
throughput sequencing, featuring broad coverage, high flexibility, high sensitivity, and high specificity, enabling multi-dimensional information output including quantitative, qualitative, and in-depth analysis. Through the probe-targeted capture "enrichment-screening" mechanism, this invention reduces probe redundancy design, minimizes background
nucleic acid interference from intestinal
flora, exhibits excellent adaptability to complex samples, and eliminates the need for complex sample pretreatment, making it suitable for large-scale epidemiological detection.