CCP peptide segment, antigen containing CCP peptide segment, reagent, kit and application

Abstract

The invention provides a CCP peptide segment, an antigen containing the CCP peptide segment, a reagent, a kit and an application. The CCP peptide has a structure shown as NH2-S3-S1-X-S2-S4-COOH, X isa citrulline-containing epitope region, S1 and S2 are flanking regions of the citrulline-containing epitope region, at most one of S1 and S2 is a charged amino acid residue, and S3 and S4 are amino acid residue regions connected to form a loop so as to form an amino acid residue region of the CCP peptide segment. The flanking regions of the CCP peptide segment are composed of amino acids with at most one amino acid serving as a charged amino acid, so that the intermolecular acting force can be reduced or the formation of an antigenic epitope can be avoided, the probability is reduced that thecitrulline antibody recognizes amino acid residues outside a key region to form an antigenic epitope and is recognized by other antibodies, thus the probability is reduced that the anti-cyclic citrulline peptide antibody is detected in the serum of non-RA patients, and the clinical detection specificity of the anti-cyclic citrulline peptide antibody in the diagnosis of RA diseases is improved.

Description

technical field [0001] The invention relates to the field of medical detection, in particular, to a CCP peptide segment, an antigen, reagent, kit and application comprising the same. Background technique [0002] Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The main clinical manifestations are symmetrical, chronic, and progressive arthritis, chronic inflammation of the synovium, hyperplasia, formation of pannus, and invasion of articular cartilage. , subchondral bone, ligaments and tendons, etc., resulting in the destruction of articular cartilage and joint capsule, eventually leading to joint deformity and loss of function. Rheumatoid arthritis is more common in middle-aged women, and the prevalence in my country is about 0.32% to 0.36%. [0003] In recent years, with the deepening of RA research, it has been gradually discovered that antiperipheral factor (APF), antikeratin antibody (AKA) and antifilaggrin antibody (AFA) in serum of RA patients ...

AI Technical Summary

Problems solved by technology

[0008] The main purpose of the present invention is to provide a CCP peptide, antigen containing it, reagent, kit and application, to solve the problem of low detection specificity of CCP antigen in the prior art

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