CCP peptide, antigen comprising it, reagent, kit and application

A kit and peptide technology, applied in the field of CCP peptides, can solve the problem of low specificity of CCP antigen detection, and achieve the effects of ensuring clinical detection sensitivity, optimizing performance, reducing background and non-specific adsorption

Active Publication Date: 2021-06-04
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The main purpose of the present invention is to provide a CCP peptide, antigen containing it, reagent, kit and application, to solve the problem of low detection specificity of CCP antigen in the prior art

Method used

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  • CCP peptide, antigen comprising it, reagent, kit and application
  • CCP peptide, antigen comprising it, reagent, kit and application
  • CCP peptide, antigen comprising it, reagent, kit and application

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Embodiment Construction

[0029] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below in conjunction with examples.

[0030] Explanation of terms:

[0031] Citrulline (Citrulline, Cit), Glutamic acid (Glu), CCP (cyclic citrulline peptide), Anti-CCP (anti-cyclic citrulline antibody), RA (rheumatoid arthritis), CLIA (chemiluminescent immunoassay), BSA (bovine serum albumin), NaN3 (sodium azide), ABEI [N-(4-aminobutyl)-N-ethyl isoluminol], HRP (Horseradish Peroxidase, spicy root peroxidase), acridinium ester (Acridinium Ester), FITC (fluorescein isothiocyanate), DCC (N, N-dicyclohexylcarbodiimide), ELISA (enzyme-linked immunosorbent assay), Immunofluorescence (ABS), immunochromatography (ICA) bovine serum albumin (BSA), cationic bovine serum albumin (cBSA), hemocyanin (KLH), ovalbumin (OVA), human serum albumin (HSA)

[0032] As mention...

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Abstract

The invention provides a CCP peptide segment, an antigen comprising it, a reagent, a kit and application thereof. The CCP peptide has NH 2 ‑S 3 ‑S 1 ‑X‑S 2 ‑S 4 The structure shown by ‑COOH, X is the epitope region containing citrulline, S 1 and S 2 is the flanking region of the antigenic epitope region containing citrulline, S 1 and S 2 At most one of them is a charged amino acid residue, S 3 and S 4 is the region of amino acid residues used for ligation to form a loop to form the CCP peptide. The flanking region of the CCP peptide is composed of at most one amino acid as a charged amino acid, which can reduce the intermolecular force or avoid the formation of antigenic epitopes, and reduce the formation of antigenic epitopes by amino acid residues outside the key region of citrulline antibody recognition. The probability of being recognized by other antibodies, thereby reducing the detection probability of anti-cyclic citrullinated peptide antibodies in the serum of non-RA patients, and improving the specificity of clinical detection of anti-cyclic citrullinated peptide antibodies in the diagnosis of RA diseases.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a CCP peptide, an antigen containing it, a reagent, a kit and an application. Background technique [0002] Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The main clinical manifestations are symmetrical, chronic, and progressive arthritis. Chronic inflammation of the synovial membrane, hyperplasia, pannus, and invasion of articular cartilage , subchondral bone, ligaments and tendons, etc., causing damage to articular cartilage and joint capsule, eventually leading to joint deformity and loss of function. Rheumatoid arthritis is more common in middle-aged women, and the prevalence rate in my country is about 0.32% to 0.36%. [0003] In recent years, with the in-depth research on RA, antiperipheral factor (APF), antikeratin antibody (Antikeratin Antibody, AKA) and antifilaggrin antibody (Antifilaggrin Antibody, AFA) in the serum of RA patients have gradua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08G01N33/68C07K19/00
Inventor 袁锦云张云甘兴陈顺利毛龙郑瑞娜
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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