Application of lumbrukinase in treating carotid atherosclerosis

A lumbrokinase and earthworm technology, which is used in the application field of lumbrokinase for preparing medicines for treating carotid atherosclerosis

Active Publication Date: 2010-06-02
BEIJING BAIAO PHARMA
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no clinical method and drug applicati

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of lumbrukinase in treating carotid atherosclerosis
  • Application of lumbrukinase in treating carotid atherosclerosis
  • Application of lumbrukinase in treating carotid atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] The preparation method of embodiment 1 lumbrokinase dry powder (1)

[0086] 1) Earthworm cleaning: After washing 10 kg of live open-air red worms with water, add 0.2 kg of cotton white sugar per kg of earthworms, stir evenly at room temperature, and let it stand for 40 minutes to stimulate the earthworms to excrete the secretions in their digestive tracts. Spit it out, and then wash off the sugar and dirt on the earthworms with warm water at 30°C;

[0087] 2) Pulping: add water of 1 times the volume of the earthworm, and use a high-pressure homogenizer to crush the earthworms cleaned in step 1) to make a homogenate;

[0088] 3) slag removal: the homogenate obtained in step 2) is filtered through a stainless steel filter with a screen aperture of 5 mm to remove the coarse slag in the homogenate;

[0089] 4) clarification: after step 3) the homogenate liquid after deslagging is processed by a high-speed centrifuge to obtain a clarified liquid;

[0090] 5) Separation: us...

Embodiment 2

[0095] The preparation method of embodiment 2 lumbrokinase dry powder (2)

[0096] Cleaning: After washing 10 kg of live open-air red worms with water, add sodium chloride at a rate of 0.3 kg of sodium chloride per kg of earthworms, stir evenly at room temperature and let stand for 40 minutes to stimulate the earthworms to excrete Spit out the secretions, and then wash off the sugar and dirt on the earthworms with warm water at 40°C.

[0097] Pulping: crush the earthworms with a bead mill, add water (the amount of water added is 8 times the volume of the earthworms) to make a homogenate; make a homogenate.

[0098] Removal of slag: Use a centrifuge to remove the coarse slag in the homogenate, and then process the homogenate after deslagging with a high-speed centrifuge to obtain a clarified liquid.

[0099] Separation: Use an ion exchange chromatography column to separate the earthworm clarified liquid, elute with 0.01M acetate, collect the active peak part of the chromatogra...

Embodiment 3

[0104] The preparation method of embodiment 3 lumbrokinase dry powder (3)

[0105] Cleaning: After washing 10 kg of live open-air red worms with water, add cotton white sugar at a rate of 0.1 kg of sugar per kg of earthworms, stir evenly at room temperature and let stand for 40 minutes to stimulate the earthworms to excrete the secretions in their digestive tracts Spit it out, and then wash off the sugar and dirt on the earthworms with warm water at 30°C.

[0106] Pulping: Crush the earthworms with a high-pressure homogenizer, add water to make a homogenate.

[0107] Removal of slag: Use a stainless steel filter with a pore size of 2 mm to filter out the coarse slag in the homogenate, and then process the homogenate after deslagging with a high-speed centrifuge to obtain a clarified liquid.

[0108] Separation: Use an affinity chromatography column to separate the earthworm clarified liquid, elute with 0.5M acetate, collect the active peak part of the chromatographic effluent...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides lumbrukinase and application thereof for preparing a medicament treating carotid atherosclerosis and a medicament containing the lumbrukinase as an active component.

Description

field of invention [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of lumbrokinase in the preparation of drugs for treating carotid atherosclerosis. Background of the invention [0002] Atherosclerosis (AS) is a general term for several diseases in which the arterial wall thickens and loses elasticity, and is the most common and important type of arteriosclerosis. The main lesion feature of atherosclerosis is the subintimal lipid deposition in some parts of the artery, accompanied by the proliferation of smooth muscle cells and fibrous matrix components, and gradually develops into atherosclerotic plaque. [0003] It is a lesion characterized by patchy subintimal thickening (atheroma) of medium and large arteries that reduces or blocks blood flow, thickening and hardening of the arterial wall at the site of the plaque, and After tissue necrosis, it combines with deposited lipids to form atherosclerosis, which is calle...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/48A61K38/48A61P9/10
Inventor 王砺李昕高毓涛
Owner BEIJING BAIAO PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap