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Linear epitope minimum motif peptides of PPRV (peste des petits ruminants virus) N (nucleocapsid) protein

A technology of PPR and antigenic epitope, which is applied in the fields of immunology and biomedicine, and can solve the problem that the N protein of PPR virus has not been reported.

Active Publication Date: 2015-04-29
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on epitope mapping and epitope motif identification of Peste des petits ruminant virus N protein has not been reported so far

Method used

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  • Linear epitope minimum motif peptides of PPRV (peste des petits ruminants virus) N (nucleocapsid) protein
  • Linear epitope minimum motif peptides of PPRV (peste des petits ruminants virus) N (nucleocapsid) protein
  • Linear epitope minimum motif peptides of PPRV (peste des petits ruminants virus) N (nucleocapsid) protein

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Embodiment Construction

[0057] The inventors used the improved biosynthetic short peptide method and anti-recombinant protein polyclonal antibody to provide a full-sequence fine linear B-cell epitope map for the highly immunogenic nucleocapsid protein (N) of the Tibetan strain of PPRV; and, also Epitope maps broadly applicable to a variety of PPRV viruses are provided. The invention provides an effective approach for developing a novel PPRV multi-epitope chimeric peptide vaccine and a high-sensitivity detection method, and provides a basis for subsequent research on the linear B cell epitope group of all encoded proteins of PPRV.

[0058] the term

[0059] As used herein, "isolated" means that the material is separated from its original environment (if the material is native, the original environment is the natural environment). For example, polynucleotides and polypeptides in the natural state in living cells are not isolated and purified, but the same polynucleotides or polypeptides are isolated a...

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Abstract

The invention relates to linear epitope minimum motif peptides of PPRV (peste des petits ruminants virus) N (nucleocapsid) protein and an application of the linear epitope minimum motif peptides. Aiming at a Tibetan isolate PPRV N protein with high immunogenicity, an improved short peptide biosynthesis method and an anti-recombinant protein polyclonal antibody are adopted, a complete-sequence fine linear B cell epitope map is provided and comprises 20 minimum epitope motif contained epitope peptides which can be used for detecting the infection serum of the PPRV independently or in a combined manner. And further, an epitope map with broad spectrum applicable to various PPRVs is provided.

Description

technical field [0001] The invention belongs to the technical field of immunology and biomedicine, more specifically, the invention relates to a linear epitope minimal motif peptide of N protein of Peste des petits ruminants virus. Background technique [0002] Peste des petits ruminants (PPR) is an acute contagious disease that infects small ruminants caused by peste des petits ruminants virus (PPRV) (Munir, M., Role of Wild Small Ruminants in the Epidemiology of Peste Des Petits Ruminants. Transbound Emerg Dis, 2013). As a serious transnational Class A animal infectious disease, China also stipulates it as a Class I animal disease, which is the highest level of concern. The incidence of PPR in susceptible flocks is as high as 100%, and the fatality rate is 100% in severe outbreaks. Peste des petits ruminants was first discovered in Côte d'Ivoire in 1942, and then spread to Africa and most of Asia. In 2007 and 2008, two outbreaks of Peste des petits ruminants occurred in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K19/00C07K5/093C07K16/10C12N15/62G01N33/68G01N33/569A61K39/155A61P31/14
Inventor 于瑞嵩李震董世娟朱于敏李文涛樊晓明徐万祥何亚萍唐海明
Owner SHANGHAI ACAD OF AGRI SCI
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