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Linear epitope of novel coronavirus RBD specific monoclonal antibody and application

A technology of monoclonal antibody and antigen epitope, applied in the field of immunity

Pending Publication Date: 2020-11-17
CHONGQING MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese invention patent application with the application publication number CN111440229A discloses a new coronavirus T cell epitope. The patent uses the IEDB resource Class I Immunogenicity tool to predict the T cell epitope of the new coronavirus N protein, and analyzes 600 immunogenicity , 181 non-immunogenic 9mer peptides, but there are no reports of linear epitopes recognized by B cells

Method used

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  • Linear epitope of novel coronavirus RBD specific monoclonal antibody and application
  • Linear epitope of novel coronavirus RBD specific monoclonal antibody and application
  • Linear epitope of novel coronavirus RBD specific monoclonal antibody and application

Examples

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Embodiment 1

[0025] This embodiment provides a linear epitope of a novel coronavirus RBD-specific monoclonal antibody, which has the following amino acid sequence: SEQ ID NO:1, SEQ ID NO:2 and / or SEQ ID NO:3.

[0026] This embodiment also provides the application of the linear epitope of the above-mentioned novel coronavirus RBD-specific monoclonal antibody in the preparation of nucleic acid, recombinant vector, host cell, composition, vaccine, detection test paper, detection reagent or monoclonal antibody.

Embodiment 2

[0028] The difference between Example 2, Example 3 and Example 1 is that the amino acid sequences are different, and the sequences of Example 2 and Example 3 are shown in the following table:

[0029] test group Amino acid sequence (N'-C') Example 1-SEQ ID NO:1 NLCPFGEVFNATRFASVYAW Example 2-SEQ ID NO:2 NNLDSKVGGNYNYLYRLFRKSNLKP Example 3-SEQ ID NO:3 PTNGVGYQPYRVVVLSFELL

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Abstract

The invention belongs to the technical field of immunity, and particularly discloses a linear epitope, with following amino acid sequences of SEQ ID NO: 1, SEQ ID NO: 2 and / or SEQ ID NO: 3, of a novelcoronavirus RBD specific monoclonal antibody. The invention also discloses application of the linear epitope of the novel coronavirus RBD specific monoclonal antibody. The linear epitope of the novelcoronavirus RBD specific monoclonal antibody has important significance in research and development of vaccines against the COVID-19 and research, development, diagnosis, prevention and treatment ofsmall molecular drugs.

Description

technical field [0001] The invention belongs to the technical field of immunization, and in particular relates to a linear epitope of a novel coronavirus RBD-specific monoclonal antibody and its application. Background technique [0002] SARS-CoV-2 belongs to the order Nidovirales, the family Coronaviridae, and the genus Coronavirus. It is the virus with the largest genome among RNA viruses known to humans. Its length is 27 to 32 kb, and it has at least 4 main structural proteins, including spike protein (S protein), membrane protein (M protein), envelope protein (E protein) and nucleocapsid protein (N protein), the virus enters the cell depends on the S protein and S protein The receptor binding domain (RBD), the S protein has two subunits, S1 and S2, and the receptor binding site (RBD) is located on the S1 subunit, its main function is to recognize the host cell surface receptors, mediate the interaction with the host cell Fusion; N protein is a basic phosphoprotein, and ...

Claims

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Application Information

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IPC IPC(8): C07K14/165A61K39/215A61P31/14G01N33/68G01N33/569G01N33/577
CPCC07K14/005A61K39/12A61P31/14G01N33/6854G01N33/56983G01N33/577C12N2770/20022C12N2770/20034G01N2333/165G01N2469/20
Inventor 金艾顺李婷婷韩晓建王应明李胜龙王建为胡超申美莹
Owner CHONGQING MEDICAL UNIVERSITY
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