Method for detecting multiple types of ovine babesiasis and detection kit thereof

Abstract

The invention discloses a method for detecting ovine babesiasis indeterminate species, Babesia motasi LZ subspecies and Babesia motasi NB subspecies as well as a detection kit thereof. The detection method takes ovine babesiasis thrombospondin-related anonymous protein TRAP gene as a detection identifier. The kit comprises six primers shown in SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, and SEQ ID No.6, The method takes TRAP as the detection identifier, the difference between species and subspecies is large, the detection sensitivity is better than that of the current technologies. The kit can simultaneously amplify several target DNA molecules in a PCR reaction, and realizes a purpose of simultaneously discriminating and detecting a plurality of microbes in a same sample. Compared with the other molecule detection technologies, the method has the advantages of simpleness, rapidity, high efficiency and saved cost, is suitable for rapid detection of cause of disease, and can be a hotspot technology for scientific research and clinical diagnosis.

Description

technical field [0001] The invention relates to a method capable of detecting various Babesia sheep and its detection kit, in particular to a method for detecting undetermined species of Babesia sheep, Babesia mosoni LZ subspecies and Babesia mosoni NB Subspecies method and detection kit. Background technique [0002] Ovine babesiosis is a disease of the genus Babesia ( Babesia ) is a blood protozoan disease caused by a variety of Babesia parasitizing in sheep red blood cells. Infected animals show clinical symptoms such as high fever, anemia, jaundice and hemoglobinuria, and can even cause death in severe infection. Currently, the reported and named species of Babesia ovis mainly include Babesia ovis ( B.ovis ), Babesia mosoni ( B. motasi ) and Babesia roughas ( B. crassa ). Babesia ovis is the most pathogenic, followed by Babesia mosoni, and Babesia crassa is almost non-pathogenic. The vectors of Babesia ovis and Babesia mosenii are Rhizocephalus ticks ( Rhipic...

AI Technical Summary

Problems solved by technology

However, although conventional blood smear microscope detection method is regarded as the gold standard for the diagnosis of babesiosis, it is difficult to detect the pathogen under the microscope in the early stage of infection or during the persistent infection period (the infection rate in these periods is low)

At the same time, it is difficult to distinguish different Babesia species morphologically, and the operating proficiency will directly affect the accuracy of diagnosis results; currently established molecular detection methods such as PCR, LAMP, RLB and Real-time PCR cannot distinguish Mohs Babesia subspecies LZ and Babesia mosoni subspecies NB, and the operation is cumbersome. To determine the Babesia species infected by the same sample requires multiple tests, resulting in easy contamination and false positives; antibody detection techniques (such as ELISA) , prone to cross-reaction, more difficult to distinguish between different pathogenic infections

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