Application of a donkey bone glue characteristic polypeptide in detecting donkey bone glue components in animal hide glue and its products

A technology for animal skin glue and animal detection, which is applied in the fields of peptides, analytical materials, measuring devices, etc., can solve the problems of difficulty in distinguishing animal skin glue and bone glue, and can not distinguish and distinguish, and achieves rapid identification and detection, high sensitivity and characteristic. strong effect

Active Publication Date: 2022-04-12
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still great difficulties in distinguishing between animal skin glue and bone glue. For example, donkey-hide gelatin and donkey bone glue have similar components and the same type I collagen sequence, so the previous methods cannot be used to distinguish them.

Method used

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  • Application of a donkey bone glue characteristic polypeptide in detecting donkey bone glue components in animal hide glue and its products
  • Application of a donkey bone glue characteristic polypeptide in detecting donkey bone glue components in animal hide glue and its products
  • Application of a donkey bone glue characteristic polypeptide in detecting donkey bone glue components in animal hide glue and its products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1 Materials and Reagents

[0029] Samples to be tested: various pure glues, including donkey bone glue, donkey-hide gelatin, yellow gelatin, and pig skin glue, obtained by decocting donkey bone, donkey skin, cowhide, and pig skin respectively.

[0030] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0031] 2 detection method

[0032] Take 0.05g of the sample to be tested in a 25ml measuring bottle, and use 1% w / vNH 4 HCO 3 The solution (pH 8.0) was dissolved and fixed to volume, passed through a 0.22 μm microporous membrane, 300 μl of the filtrate was accurately measured, 30 μl of 2 mg / ml trypsin solution was added, and the enzymatic hydrolysis was obtained at a constant temperature of 37° C. overnight.

[0033] Take 5 μl of the enzymatic hydrolysis solution and put it into the liquid mass spectrometer for detection. Liquid phase condition: C 18 Reve...

Embodiment 2

[0036] 1 Materials and Reagents

[0037] Sample to be tested: the mixed glue sample is made by adding 5%w / w donkey bone glue to the pure glue sample in Example 1, including donkey-hide gelatin containing 5%w / w donkey bone glue, donkey-hide gelatin containing 5%w / w donkey bone glue Yellow gelatin, pig skin glue 5% w / w donkey bone glue.

[0038] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0039] 2 detection method

[0040] Take 0.05g of the sample to be tested in a 25ml measuring bottle, and use 1% w / vNH 4 HCO 3 The solution (pH 8.0) was dissolved and fixed to volume, passed through a 0.22 μm microporous membrane, 300 μl of the filtrate was accurately measured, 30 μl of 2 mg / ml trypsin solution was added, and the enzymatic hydrolysis was obtained at a constant temperature of 37° C. overnight.

[0041] Take 5 μl of the enzymatic hydrolysis solution and put it...

Embodiment 3

[0044] 1 Materials and Reagents

[0045] Animal glue samples: donkey bone glue and donkey-hide gelatin were obtained by decocting donkey bone and donkey skin respectively.

[0046] Mixed gum samples: donkey-hide gelatin with 5% w / w donkey bone glue, donkey-hide gelatin with 10% w / w donkey bone glue, donkey-hide gelatin with 20% w / w donkey bone glue, donkey-hide gelatin with 40% w / w Made with a precise blend.

[0047] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0048] 2 detection method

[0049] Take 0.05g of the sample to be tested in a 25ml measuring bottle, and use 1% w / vNH 4 HCO 3 The solution (pH 8.0) was dissolved and fixed to volume, passed through a 0.22 μm microporous membrane, 300 μl of the filtrate was accurately measured, 30 μl of 2 mg / ml trypsin solution was added, and the enzymatic hydrolysis was obtained at a constant temperature of 37° C. ...

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Abstract

The invention discloses the application of a donkey bone glue characteristic polypeptide in detecting donkey bone glue components in animal hide glue and its products. The amino acid sequence of the characteristic polypeptide of donkey bone glue is shown in SEQ ID NO.1. The polypeptide is unique to donkey bone glue, but does not contain animal skin glue such as donkey-hide gelatin, yellow gelatin, and pig skin glue. The polypeptide is detected by liquid mass spectrometry, which realizes the rapid identification and detection of donkey bone glue components in animal hide glue and its products, and can also accurately quantify the donkey bone glue components. The method of the invention has strong characteristics, high sensitivity and simple operation, and provides an effective technical means for detecting donkey bone glue components in animal hide glue and its products.

Description

technical field [0001] The invention relates to a characteristic polypeptide of donkey bone glue and its application, and also relates to a method for detecting components of donkey bone glue in animal skin glue and its products. The invention belongs to the technical field of medicine and food detection. Background technique [0002] Animal glue is a glue-like substance made from animal skin and bone, which is widely used in food, health products and medicines. Common animal skin glues include donkey-hide gelatin, yellow gelatin, pigskin glue, etc., which have their own characteristics in clinical application and need to be boiled with specified raw materials. For example, donkey-hide gelatin is boiled from donkey skin, yellow gelatin is boiled from cowhide, etc. For counterfeit. Because the appearance of all kinds of glue is very similar, and the price is very different, so in addition to the easy confusion of various glues, there are also criminals who make counterfeit p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/02G01N30/06G01N30/14G01N30/72C07K7/08
CPCG01N30/88G01N30/02G01N30/06G01N30/14G01N30/72C07K7/08G01N2030/8813G01N2030/8818G01N2030/027G01N2030/062G01N2030/146
Inventor 郭尚伟段小波周祥山田守生徐云鹏王雨华王玉娇史兆松
Owner SHAN DONG DONG E E JIAO
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