Primer pairs and kit capable of detecting and distinguishing different breeding sheep babesia

Abstract

The invention discloses primer pairs and a kit capable of detecting and distinguishing sheep morse babesia and undefined species xinjiang strains of ovine babesia. Gene sequences of the primer pair capable of detecting the sheep morse babesia disclosed by the invention are SEQ ID No.1 and SEQ ID No.2. The gene sequences of the primer pair capable of detecting the undefined species xinjiang strains of the ovine babesia are SEQ ID No.4 and SEQ ID No.5. The kit disclosed by the invention has the advantages of high sensitivity, high speed, high specificity and the like on detection; the environmental pollution can be avoided; the detection time is shortened; and the primer pairs and the kit are especially suitable for detection and diagnosis of large batch of samples with acute and subclinical infection.

Description

technical field [0001] The invention relates to a kit for rapidly detecting and distinguishing animal blood protozoan diseases. Specifically, the present invention relates to a primer pair and a detection reagent that can not only be used for detection, but also can accurately distinguish two kinds of Babesia that infect sheep distributed in my country, that is, Babesia mosoni and Babesia sheep unspecified Xinjiang strain box. Background technique [0002] Babesia in sheep is a blood protozoan disease transmitted by ticks. It is caused by Babesia ovis, Babesia moschii, Babesia crassa and unspecified Babesia ovis, causing fever and jaundice in infected animals. , anemia, hemoglobinuria, and even death. The disease is prevalent in tropical, subtropical, and temperate regions, causing serious harm to the world's animal husbandry. Babesia ovis ( Babesia. ovis ) and Babesia mosoni ( B. motasi ) is the main pathogen that causes sheep babesiosis; Babesia ovis is small in body ...

AI Technical Summary

Problems solved by technology

Moreover, Babesia that infects sheep isolated in my country, namely Babesia mosoni and unspecified Xinjiang strains of Babesia sheep are often in mixed infection or recessive infection in endemic areas, and the differential diagnosis between different pathogens has always been a problem. Technical bottleneck of epidemiological investigation of the disease

[0004] At present, the commonly used method for diagnosing the disease is blood smear staining, but it is difficult to detect the pathogen under a microscope when the infection rate

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