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PEDV immuno-chromatography test strip along with preparation method and application

A chromatographic test strip, immunodetection technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of complex operation, high diagnosis cost, long time consumption, etc., and achieve high sensitivity, short detection period, and good specificity.

Inactive Publication Date: 2018-12-21
SHENZHEN AUDAQUE DATA TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, traditional differential diagnosis methods, such as virus antigen detection, virus immunoelectron microscope observation, virus isolation and identification, etc., all require very cumbersome pre-treatment of disease materials, which not only requires a lot of work and time-consuming, but also complicated operation and high diagnostic cost. high

Method used

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  • PEDV immuno-chromatography test strip along with preparation method and application
  • PEDV immuno-chromatography test strip along with preparation method and application
  • PEDV immuno-chromatography test strip along with preparation method and application

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Embodiment

[0029] 1. Materials and equipment

[0030] (1) cells and viruses

[0031] Vero-76 passage cell line, monkey kidney passage cell line (MA-104), porcine kidney cells (PK-15), SP2 / 0-Ag14 (SP2 for short) myeloma cells, were all provided by Microbiology, School of Veterinary Medicine, South China Agricultural University. Nuclear preservation is provided by the Department of Immunology.

[0032] 2015-PEDV-6, 2016-PEDV+PROVA-1, 2016-PEDV+PRoVA-2, 2016-PRoVA-1, 2017-PEDV+PROVA+TGEV-3, TGEV-GD were all funded by Microbiology, School of Veterinary Medicine, South China Agricultural University Nuclear preservation is provided by the Department of Immunology.

[0033] "Kexiening", porcine transmissible gastroenteritis, porcine epidemic diarrhea dual inactivated vaccine (WH-1 strain + AJ1102 strain) is a product of Wuhan Keqian Biotechnology Co., Ltd.; porcine viral diarrhea triple live vaccine, porcine Infectious gastroenteritis, porcine epidemic diarrhea, and porcine rotavirus (G5 typ...

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Abstract

The present application discloses a PEDV immuno-chromatography test strip along with preparation method and application. The PEDV immune-chromatography test strip of the present application comprises:the sample pad and the chromatography strip on support material; the assay band coated with anti-PEDV antibody; the quality control band coated with goat anti-mouse IgG antibody; and the sample pad sprayed with quantum dot-labeled anti-PEDV antibody probe. The PEDV immuno-chromatography test strip of the present application employed quantum dot-labeled anti-PEDV antibody probe for bioassay. It combines the high sensitivity of fluorescent quantum dots and the high specificity of antigen and antibody, so that the immuno-chromatography test strip has the advantages of high sensitivity, good specificity and short testing period. It is good for on-site test and is suitable for screening and monitoring the porcine epidemic diarrhea virus.

Description

technical field [0001] The invention relates to the technical field of immunochromatographic detection, in particular to a PEDV immunochromatographic test strip and its preparation method and application. Background technique [0002] Porcine epidemic diarrhea (PED) is a severe porcine infectious disease caused by porcine epidemic diarrhea virus (PEDV). Porcine epidemic diarrhea is prevalent in many countries, and it is becoming more and more serious. Rapid diagnosis of PEDV is the premise and basis for preventing and controlling the epidemic of PED. All countries have strengthened the monitoring of epidemic diarrhea. However, traditional differential diagnosis methods, such as virus antigen detection, virus immunoelectron microscope observation, virus isolation and identification, etc., all require very cumbersome pre-treatment of disease materials, which not only requires a lot of work and time-consuming, but also complicated operation and high diagnostic cost. high. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/54306G01N33/56983
Inventor 李军李汉锐秦智锋孙洁郑晓聪陈兵刘建利王津津于力
Owner SHENZHEN AUDAQUE DATA TECH
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