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Reagent card for detecting lily symptomless virus by adopting colloidal gold immunochromatographic assay and preparation method

An immunochromatographic detection and recessive virus technology, which is applied in the direction of measuring devices, analysis materials, biological material analysis, etc., can solve problems such as difficult to meet the needs of virus detection, and achieve rapid detection, high detection sensitivity, and convenient portability

Active Publication Date: 2014-10-29
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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AI Technical Summary

Problems solved by technology

But so far there are no relevant reports on the detection of LSV by colloidal gold immunochromatography at home and abroad, and there is no commercialized LSV colloidal gold immunochromatography detection reagent card, which is difficult to meet the virus detection needs of domestic ornamental and edible lilies.

Method used

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  • Reagent card for detecting lily symptomless virus by adopting colloidal gold immunochromatographic assay and preparation method
  • Reagent card for detecting lily symptomless virus by adopting colloidal gold immunochromatographic assay and preparation method

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Embodiment Construction

[0020] Such as figure 1 and figure 2The shown LSV colloidal gold immunochromatography detection reagent card includes a reagent card slot 1, a backing plate 10, a sample pad 6, a colloidal gold binding pad 7, a nitrocellulose membrane 8, and a water-absorbing filter paper 9, wherein the reagent card slot 1 includes an upper The shell and the lower shell, the upper shell and the lower shell are connected by buckles, the upper shell is provided with a first hole 2 and a second hole 3, the sample pad 6 is placed under the first hole 2, and the nitrocellulose membrane 8 Placed below the second hole 3, the colloidal gold binding pad 7 contains a gold standard probe, the liner 10 is fixed in the reagent card slot 1, the sample pad 6, the colloidal gold binding pad 7, the nitrocellulose membrane 8 and the absorbent filter paper 9 Arranged in sequence and connected to the upper surface of the liner 10, the nitrocellulose membrane 8 is provided with a detection line 4 and a control l...

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Abstract

The invention relates to a reagent card for detecting lily symptomless virus by adopting colloidal gold immunochromatographic assay and a preparation method. The reagent card comprises a reagent card slot, a lining board, a sample pad, a colloidal gold combining pad, a nitrocellulose membrane and suction filter paper, wherein the colloidal gold combining pad contains a golden probe, the lining board is fixed in the reagent card slot, the sample pad, the colloidal gold combining pad, the nitrocellulose membrane and the suction filter paper are arranged and connected to the upper surface of the lining board in sequence, a detection line and a contrast line are formed on the nitrocellulose membrane, and a rabbit anti-LSV polyclonal antibody and a sheep anti-rabbit IgG purification antibody respectively coat the detection line and the contrast line. The preparation method of the reagent card comprises the following main steps: coating an immunochromatographic membrane by the rabbit anti-LSV polyclonal antibody, and then preparing the colloidal gold combining pad. The reagent card has the advantages of rapidity in detection, high accuracy, strong specificity, simplicity and convenience in operation and no need of special equipment and instruments.

Description

technical field [0001] The invention relates to a detection reagent card and a preparation method thereof, in particular to a reagent card for rapidly detecting lily hidden syndrome virus (LSV) by colloidal gold immunochromatography and a preparation method thereof. Background technique [0002] Lily (Lilium spp) is a traditional medicinal and edible plant, and has become one of the most important flower bulbs in the world. The Netherlands is the largest producer of lily bulbs in the world. In 2005, the production area of ​​Dutch lily bulbs reached 3800hm 2 , accounting for 72% of the total global area, produces 2.21 billion lily bulbs every year, of which about 100 million are exported to China. Cut flower lily occupies the fourth place in the output value of my country's flower industry. At the same time, lily is also an important economic crop as food and medicine. Lanzhou edible lily is famous at home and abroad for its white color, large shape, sweet taste, and rich n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/558G01N33/56983G01N2333/08
Inventor 张玉宝王亚军谢忠奎王若愚郭志鸿张丽华
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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