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Immunochromatographic test paper for rapid combined diagnosis of neocoronavirus, influenza A and influenza B and preparation method thereof

A technology of immunochromatography detection and detection test paper, which is applied in the field of immunodiagnosis, and can solve the problems of long detection time and single detection items

Pending Publication Date: 2020-12-25
HANGZHOU ALLTEST BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems of long time-consuming and single detection items in the detection of novel coronavirus, the present invention provides an immunochromatographic detection test paper for rapid joint diagnosis of new crown / flu A / flu B and its preparation method, which can simultaneously detect novel coronavirus N / S protein, Influenza A virus and Influenza B virus, the detection test paper adopts the way of antibody detection antigen, the antigen has no incubation period, which is more accurate than the product of detection antibody, the accuracy of detection antigen is higher than that of detection antibody, the detection efficiency is high, and the preparation process Simple

Method used

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  • Immunochromatographic test paper for rapid combined diagnosis of neocoronavirus, influenza A and influenza B and preparation method thereof
  • Immunochromatographic test paper for rapid combined diagnosis of neocoronavirus, influenza A and influenza B and preparation method thereof
  • Immunochromatographic test paper for rapid combined diagnosis of neocoronavirus, influenza A and influenza B and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of preparation method of the immunochromatographic detection test paper of fast joint diagnosis new crown / fluid A / fluid B, comprises the following steps, step 1-preparation of new crown detection test paper,

[0049] 1) Preparation of test line T3 and quality control line C-4: Dilute the anti-new coronavirus N protein monoclonal antibody mixed with anti-new coronavirus S protein monoclonal antibody and streptavidin conjugate solution respectively, and then dilute them at 1.0 The speed of μl / cm is sprayed on the nitrocellulose membrane as the detection line T3 and the quality control line C-4 respectively, and placed in 36°C for drying to obtain the detection line T3 and the quality control line C-4; the detection line T3 The coated coating uses anti-new coronavirus N protein monoclonal antibody and anti-new coronavirus S protein monoclonal antibody concentrations to be 0.5mg / ml and 0.5mg / ml respectively; the quality control line C-4 coated chain The concentration...

Embodiment 2

[0058] The difference with Example 1 is the preparation of step 2-A flow / B flow detection test paper,

[0059] 1) Preparation of detection line A10, detection line B11 and quality control line C28: Dilute the anti-influenza virus monoclonal antibody, anti-influenza virus monoclonal antibody and goat anti-mouse IgG antibody solution respectively in 1.05 μl Spray points at a speed of / cm on the nitrocellulose membrane as the detection line A10, the detection line B11 and the quality control line C-8 respectively, and place them in 36.5°C for drying to obtain the detection line and the quality control line; the detection line A10 packs The coated anti-influenza virus monoclonal antibody concentration is 1.0mg / ml; the detection line B11 coated with anti-influenza virus monoclonal antibody concentration is 1.0mg / ml; the quality control The concentration of goat anti-mouse IgG antibody coated with line C28 is 1.5mg / ml;

[0060] 2) Preparation of the gold-labeled probe treatment pad...

Embodiment 3

[0062] The difference with Example 1 is the preparation of step 2-A flow / B flow detection test paper,

[0063] 1) Preparation of detection line A10, detection line B11 and quality control line C28: Dilute anti-influenza virus monoclonal antibody, anti-influenza virus monoclonal antibody and goat anti-mouse IgG antibody solution respectively in 1.1 μl Spray points at a speed of / cm on the nitrocellulose membrane as the detection line A10, the detection line B11 and the quality control line C-8 respectively, and place them in 37°C for drying to obtain the detection line and the quality control line; the detection line A10 packs The coated anti-influenza virus monoclonal antibody concentration is 1.5mg / ml; the detection line B11 coated anti-influenza virus monoclonal antibody concentration is 1.5mg / ml; the quality control The concentration of goat anti-mouse IgG antibody coated with line C28 is 2.0mg / ml;

[0064] 2) Preparation of gold-labeled probe treatment pad 9: Dilute the p...

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Abstract

The invention relates to the field of immunodiagnosis, and provides immunochromatographic test paper for rapid combined diagnosis of neocoronavirus, influenza A and influenza B aiming at the problemsof long time consumption and single project of neocoronavirus detection. The immunochromatographic test paper comprises neocoronavirus test paper and influenza A / influenza B test paper, wherein each of the neocoronavirus test paper and the influenza A / influenza B test paper comprises a PVC bottom plate, a modified nitrocellulose membrane, a water absorption pad and a sample pad; the neocoronavirustest paper comprises a latex labeled treatment pad; a detection line T coated with an anti-neocoronavirus protein monoclonal antibody and a quality control line C coated with a streptavidin conjugateare arranged on the modified nitrocellulose membrane; a treatment pad of the influenza A / influenza B test paper is a gold-labeled probe treatment pad; and a detection line A coated with an anti-influenza A virus monoclonal antibody, a detection line B coated with an anti-influenza B virus monoclonal antibody and a quality control line C2 coated with a goat anti-mouse IgG antibody are arranged onthe modified nitrocellulose membrane. The detection efficiency of detecting antigens by using the antibodies is high. The invention also provides a preparation method of the test paper.

Description

technical field [0001] The invention relates to the technical field of immunodiagnosis, in particular to an immunochromatographic detection test paper for rapid combined diagnosis of COVID-19 / flu A / flu B and a preparation method thereof. Background technique [0002] The Coronavirus Study Group (CSG) of the International Committee on Taxonomy of Viruses recommends that the 2019 novel coronavirus (Novel coronavirus pneumonia, NCP) COVID-19 be named "severe acute respiratory syndrome coronavirus" on the basis of a systematic analysis of related coronaviruses. 2 (SARS-CoV-2)”. Since the outbreak of pneumonia caused by the new coronavirus, a large number of PCR nucleic acid and IgM / IgG detection reagents have appeared on the market, but there are almost no detections for antigens. For example, a patent with publication number CN111454839A discloses an integrated device for nucleic acid preparation and PCR detection of new coronavirus, including: a housing; a sample processing m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/532G01N33/577G01N33/569G01N33/58G01N21/78
CPCG01N33/56983G01N33/558G01N33/577G01N33/585G01N33/532G01N21/78G01N2333/11G01N2333/165G01N2469/10
Inventor 陆维克吴晓诚陈金树孙晓笛沈红萍
Owner HANGZHOU ALLTEST BIOTECH
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