Immunochromatographic milk PEDV (porcine epidemic diarrhea virus) test strip and preparation method and application thereof

Abstract

The invention discloses an immunochromatographic milk PEDV (porcine epidemic diarrhea virus) test strip and a preparation method and application thereof. The immunochromatographic milk PEDV test stripcomprises a sample pad and a chromatographic strip which are overlapped onto a supporting material, wherein a detection line and a quality control line are arranged on the chromatographic strip; inactivated whole PEDV viruses are fixed to the detection line; a sheep anti-rabbit IgG antibody is fixed to the quality control line; a quantum dot-labeled S1 recombinant protein probe and a quantum dot-labeled rabbit IgG probe are sprayed on the sample pad. The immunochromatographic test strip fully combines the characteristics of the high sensitivity of fluorescent quantum dots and the antigen-antibody specificity, and can detect presence of an IgA antibody in a body fluid of an infected or immunized animal or human body or not, such as detection of the IgA antibody in milk of a sow infected orimmunized by the PEDV. The immunochromatographic test strip is high in detection sensitivity, good in specificity and short in cycle, can meet on-site detection needs, and is suitable for molecular biological detection of the porcine epidemic diarrhea viruses and large-scale epidemiological investigation.

Description

technical field [0001] The invention relates to the technical field of immunochromatographic detection, in particular to a PEDV immunological detection chromatographic test strip in milk and a preparation method and application thereof. Background technique [0002] Porcine epidemic diarrhea (PED) is a severe porcine infectious disease caused by PEDV (porcine epidemic diarrhea virus). PED is prevalent in many countries, and it is becoming more and more serious. All countries have strengthened the monitoring of epidemic diarrhea. [0003] The researchers conducted epidemiological investigations on the occurrence and prevalence of piglet diarrheal disease in multiple regions in 2015 and 2017, and showed that piglet diarrheal disease occurred and was popular in multiple regions this time, and the main pathogen was porcine epidemic diarrhea virus. Followed by infectious gastroenteritis, as well as porcine rotavirus disease, Kobu virus disease, etc., some farms also have seconda...

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Problems solved by technology

[0004] The rapid diagnosis of PEDV is the premise and basis for preventing and controlling the prevalence of PED. However, traditional differential diagnosis methods, such as virus antigen detection, virus immuno-electron microscope observation, virus isolation and identification, etc., require very cumbersome pre-treatment of disease materials. , not only the workload is heavy and time-consuming, but also the operation is complicated and the diagnosis cost is high

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