Canine parvovirus colloidal gold immunochromatography test strip and preparation method

A technology for immunochromatographic detection and canine parvovirus, which is applied in measurement devices, analytical materials, biological material analysis, etc., can solve problems such as limitations, and achieve the effects of simple operation, rapid detection, and high detection accuracy.

Inactive Publication Date: 2014-02-26
WUHAN CHOPPER BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Serological methods established in my country for detecting CPV antibodies include hemagglutination test, hemagglutination inhibition test, agar diffusion test, etc. chemical response, so these methods are often limited in practical application
Enzyme-linked immunosorbent assay (ELISA) can detect CPV antigen and CPV antibody; PCR detection has high sensitivity and specificity, and can distinguish wild virus infection and vaccine immunity, but both techniques require special equipment

Method used

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  • Canine parvovirus colloidal gold immunochromatography test strip and preparation method
  • Canine parvovirus colloidal gold immunochromatography test strip and preparation method
  • Canine parvovirus colloidal gold immunochromatography test strip and preparation method

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Experimental program
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Effect test

Embodiment 1

[0027] The schematic diagram of the structure of the canine parvovirus colloidal gold immunochromatographic detection test strip is as follows figure 1 As shown, the horizontal plane of the test strip is sequentially from right to left the sample absorption area 1, the gold label probe area 2, the immobilized antibody area 3 and the water absorption area 4, which are laid on the bottom plate 5 and partially overlap each other; the sample absorption area 1, The materials of gold standard probe area 2, immobilized antibody area 3, water absorption area 4 and bottom plate 5 are glass fiber membrane, polyester membrane, nitrocellulose membrane, water absorption filter paper and polyethylene plate respectively. The gold-labeled probe area 2 is coated with colloidal gold-labeled anti-canine parvovirus monoclonal antibody F1, and the labeling amount is 4 μg / mL; the solid-phase antibody area 3 (from the gold-labeled probe area to the water-absorbing area) has Detection line 31 and con...

Embodiment 2

[0035] Except that the amount of anti-canine parvovirus monoclonal antibody B6 coated on detection line 31 was 1.5 μg / cm; the amount of goat anti-mouse IgG coated on control line 32 was 1.5 μg / cm; the colloid coated with gold standard probe area 2 The labeling amount of gold-labeled anti-canine parvovirus monoclonal antibody F1 is 4 μg / mL, and colloidal gold-labeled anti-canine parvovirus monoclonal antibody F1 method: take 20 mL of colloidal gold solution with a radius of 25 nm and anti-canine parvovirus monoclonal antibody F180 μg , under the condition of pH 9.0, it was combined by magnetic stirring and shaking, PEG-20000 was added as a stabilizer, and the final mass concentration of PEG-20000 was 20%, and the unbound anti-canine parvovirus monoclonal antibody F1 was removed by centrifugation And unstabilized colloidal gold particles and aggregates thereof, except for the colloidal gold-F1 complex, the dark red precipitate at the bottom of the centrifuge tube is the same as i...

Embodiment 3

[0037] Except that the amount of anti-canine parvovirus monoclonal antibody B6 coated on detection line 31 was 4.8 μg / cm; the amount of goat anti-mouse IgG coated on control line 32 was 4.8 μg / cm; the colloid coated with gold standard probe area 2 The labeling amount of gold-labeled anti-canine parvovirus monoclonal antibody F1 is 10 μg / mL, and colloidal gold-labeled anti-canine parvovirus monoclonal antibody F1 method: take 20 mL of colloidal gold solution with a radius of 40 nm and anti-canine parvovirus monoclonal antibody F1 200 μg , under the condition of pH 9.0, it was combined by magnetic stirring and shaking, PEG-20000 was added as a stabilizer, and the final mass concentration of PEG-20000 was 10%, and the unbound anti-canine parvovirus monoclonal antibody F1 was removed by centrifugation And unstabilized colloidal gold particles and aggregates thereof, except for the colloidal gold-F1 complex, the dark red precipitate at the bottom of the centrifuge tube is the same a...

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Abstract

The invention discloses a canine parvovirus colloidal gold immunochromatography test strip and a preparation method. The test strip comprises a sample absorbing area, a gold label probe area, an immobilized antibody area, a water absorbing area and a bottom plate, wherein the sample absorbing area, the gold label probe area, the immobilized antibody area and the water absorbing area are sequentially paved on the bottom plate and partially overlapped each other; the gold label probe area is coated with a colloidal gold labeled anti-canine parvovirus monoclonal antibody F1; the immobilized antibody area is sequentially provided with a test line and a control line, the test line is coated with an anti-canine parvovirus monoclonal antibody B6, and the control line is coated goat anti-mouse IgG (immunoglobulin G). According to the preparation method, a glass fiber membrane, a polyester membrane coated with the anti-canine parvovirus monoclonal antibody F1, a nitrocellulose membrane coated with the test line and the control line, and water absorbing filter paper are assembled onto a polyethylene plate to obtain the canine parvovirus colloidal gold immunochromatography test strip. The test strip is fast to test, high in accuracy rate, high in specificity, and simple and convenient to carry and operate.

Description

technical field [0001] The invention relates to a detection test strip and a preparation method, in particular to a canine parvovirus colloidal gold immune chromatography detection test strip and a preparation method. Background technique [0002] Canine parvovirus (Canine Parvovirus, CPV) is a member of the genus Parvovirus, a single-stranded small DNA virus. Virus particles are equiaxed and symmetrical icosahedrons, round or hexagonal in appearance, without envelopes, with a diameter of 21nm to 24nm and a sedimentation coefficient of 23S to 27S. In 1977, for the first time, CPV was isolated from sick dogs suffering from enteritis. The sick dogs were mainly characterized by vomiting, hemorrhagic enteritis, and leukopenia. The puppies were highly susceptible and could cause myocarditis in puppies; (MCV) was named CPV-2, and was subsequently discovered in canines in many countries and regions, with a morbidity rate of 50% to 100% and a mortality rate of 0% to 50%. The econo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/56983G01N33/532G01N33/558G01N33/577G01N2333/015
Inventor 刘洁牟林琳廖园园何文辉王威漆世华朱薇温文生谢红玲冯钊
Owner WUHAN CHOPPER BIOLOGY
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