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Anti-rabies virus IgG antibody colloidal gold immunochromatographic assay reagent plate and preparation method

A technology for immunochromatographic detection and rabies virus, which is applied in measuring devices, analysis materials, instruments, etc., can solve the problems of protein purification and renaturation difficulties, and the detection is limited by species, and achieve rapid detection, easy operation, and accurate detection high rate effect

Active Publication Date: 2010-12-29
WUHAN CHOPPER BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The methods involved in the above literatures have the disadvantages that the detection is limited by the species and the protein purification and renaturation are difficult.

Method used

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  • Anti-rabies virus IgG antibody colloidal gold immunochromatographic assay reagent plate and preparation method
  • Anti-rabies virus IgG antibody colloidal gold immunochromatographic assay reagent plate and preparation method
  • Anti-rabies virus IgG antibody colloidal gold immunochromatographic assay reagent plate and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: as figure 1 , figure 2 As shown, the anti-rabies virus IgG antibody colloidal gold immunochromatography detection reagent plate, the horizontal plane of the reagent plate is in order from bottom to top: sample absorption area 1, gold standard SPA area 2, immobilized antigen, antibody area 3 and water absorption area 4 , the sample absorption area 1 is paved with a glass fiber membrane on a polyethylene plate and a polyvinyl chloride lining film 5, and a gold standard probe polyester membrane 7 and a nitrocellulose membrane 6 are laid on the glass fiber membrane in sequence; The membrane 6 is coated with a detection line 31 and a control line 32, wherein the detection line 31 side is affixed with a gold-labeled probe polyester film 7, the control line 32 side is affixed with a water-absorbing filter paper 8, and the detection line 31 is coated with a rabies virus purified antigen , the coating amount is 0.1 μg protein; the control line 32 is coated with a...

Embodiment 2

[0031]Except that the detection line 31 is coated with 10 μg protein; the control line 32 is coated with anti-SPA purified antibody, the coating volume is 5 μg protein, the suitable SPA labeling amount of the gold-labeled probe is 10 μg / ml, and the method for colloidal gold-labeled SPA: Take 20ml of colloidal gold with a radius of 5nm and 200μg of SPA, combine them by magnetic stirring and shaking under the condition of pH 7.0, add bovine serum albumin (BSA) as a stabilizer, and make the final mass concentration of BSA 0.1%, Adopt centrifugation to remove unbound SPA and unstabilized colloidal gold particles and aggregates thereof, except that the dark red precipitate at the bottom of the centrifuge tube is the colloidal gold-SPA complex, and the rest are the same as in Example 1.

Embodiment 3

[0033] Except that the detection line 31 is coated with 5 μg protein; the control line 32 is coated with anti-SPA purified antibody, the coating volume is 10 μg protein, the suitable SPA labeling amount of the gold-labeled probe is 5 μg / ml, and the method for colloidal gold-labeled SPA: Take 20ml of colloidal gold with a radius of 20nm and 100 μg of SPA, combine them by magnetic stirring and vibration under the condition of pH 7.0, add bovine serum albumin (BSA) as a stabilizer, and make the final mass concentration of BSA 2.0%, Adopt centrifugation to remove unbound SPA and unstabilized colloidal gold particles and aggregates thereof, except that the dark red precipitate at the bottom of the centrifuge tube is the colloidal gold-SPA complex, and the rest are the same as in Example 1.

[0034] How to use the anti-rabies virus IgG antibody rapid detection kit:

[0035] When testing, take a small amount of serum from the subject, drop it on the reagent plate, and compare the col...

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Abstract

The invention relates to an anti-rabies virus IgG antibody colloidal gold immunochromatographic assay reagent plate and a preparation method. Glass fiber paper and cellulose nitrate films are laid on a polyethylene plate and a polyvinyl chloride substrate film; the cellulose nitrate film is coated with an assay line and a contrast line; and a gold-labeled probe polyester film is adhered on the assay line side; water-absorbing filter paper is adhered on the contrast line side, wherein the assay line is coated with rabies virus purified antigen, and the contrast line is coated with anti-SPA purified antigen. The reagent plate of the invention has the advantages of rapid detection, high detection accuracy, high specificity, convenient carrying, simple and convenient operation and can be usedfor detecting rabies virus antibodies of various animals and human. The assay reagent plate can be stored at the normal temperature for 1 year without special equipment or apparatus and has high assay repeatability.

Description

technical field [0001] The invention relates to a detection reagent plate and a preparation method, in particular to an anti-rabies virus IgG antibody colloidal gold immunochromatography detection reagent plate and a preparation method. Background technique [0002] Rabies is a zoonotic infectious disease caused by Rabies virus (RV), and its main hosts are dogs, cats and other animals. The typical symptom is hydrophobia, also known as: hydrophobia. The disease is extremely dangerous, and the case fatality rate is almost 100%. With the development of the economy and the improvement of people's living standards, the number of pet dogs is not only increasing, but also the area of ​​activity of dogs is constantly expanding. According to the information obtained from the pet medical market, people are eager to know whether pet dogs can gain protection after being vaccinated against rabies. However, the current detection of rabies virus antibody has not been widely used due to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/544
Inventor 廖园园刘汉平刘洁王威彭杏薛霜漆世华温文生
Owner WUHAN CHOPPER BIOLOGY
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