Colloidal gold immunochromatography test paper for combined diagnosis of COVID-19 and mycoplasma pneumoniae and preparation method thereof
A COVID-19, immunochromatographic detection technology, applied in the field of immunodiagnosis, can solve the problem of mycoplasma pneumonia chest X-ray examination is not characteristic, unable to make a diagnosis and other problems
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[0055] Example 1
[0056] The IgM-coated mouse anti-human IgM antibody concentration of the test line is 0.1 mg / ml, and the concentration of the mouse anti-human IgG antibody coated with the test line IgG is 0.1 mg / ml; the quality control line C 4 coated The concentration of goat anti-mouse IgG antibody is 0.5 mg / ml. The colloidal gold conjugate treatment pad 2 is coated with the neocoronavirus N protein recombinant antigen-colloidal gold conjugate, the neocoronavirus N protein recombinant antigen-colloidal gold conjugate has a diameter of 40nm-100nm, and the neocoronavirus recombinant antigen The concentration is 1mg / ml.
[0057] In the preparation method of the colloidal gold immunochromatographic test paper for the joint diagnosis of COVID-19 and Mycoplasma pneumoniae, the A test paper specifically includes the following steps:
[0058] A) Preparation of test line T and quality control line C: Dilute the mouse anti-human IgM, mouse anti-human IgG, and goat anti-mouse IgG antibod...
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[0067] Example 2
[0068] The difference from Example 1 is that the concentration of mouse anti-human IgM antibody coated with IgM of the detection line is 0.4 mg / ml, and the concentration of mouse anti-human IgG antibody coated with IgG of the detection line is 0.3 mg / ml; The concentration of goat anti-mouse IgG antibody coated with quality control line C 4 was 0.8 mg / ml. The colloidal gold conjugate treatment pad 2 is coated with the neocoronavirus N protein recombinant antigen-colloidal gold conjugate, the neocoronavirus N protein recombinant antigen-colloidal gold conjugate has a diameter of 40nm-100nm, and the neocoronavirus recombinant antigen The concentration is 3mg / ml.
[0069] In the preparation method of the colloidal gold immunochromatographic test paper for the joint diagnosis of COVID-19 and Mycoplasma pneumoniae, the A test paper specifically includes the following steps:
[0070] A) Preparation of test line T and quality control line C: Dilute the mouse anti-human I...
Example Embodiment
[0079] Example 3
[0080] The difference from Example 1 is that the concentration of mouse anti-human IgM antibody coated with IgM of the detection line is 1.0 mg / ml, and the concentration of mouse anti-human IgG antibody coated with IgG of the detection line is 1.0 mg / ml; The concentration of goat anti-mouse IgG antibody coated with quality control line C 4 was 1.2 mg / ml. The colloidal gold conjugate treatment pad 2 is coated with the neocoronavirus N protein recombinant antigen-colloidal gold conjugate, the neocoronavirus N protein recombinant antigen-colloidal gold conjugate has a diameter of 40nm-100nm, and the neocoronavirus recombinant antigen The concentration is 5mg / ml.
[0081] In the preparation method of the colloidal gold immunochromatographic test paper for the joint diagnosis of COVID-19 and Mycoplasma pneumoniae, the A test paper specifically includes the following steps:
[0082] A) Preparation of test line T and quality control line C: Dilute mouse anti-human IgM, ...
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