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Colloidal gold immunochromatography test paper for combined diagnosis of COVID-19 and mycoplasma pneumoniae and preparation method thereof

A COVID-19, immunochromatographic detection technology, applied in the field of immunodiagnosis, can solve the problem of mycoplasma pneumonia chest X-ray examination is not characteristic, unable to make a diagnosis and other problems

Inactive Publication Date: 2020-07-17
HANGZHOU ALLTEST BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The clinical manifestations and chest X-ray examination of mycoplasma pneumonia are not characteristic, and the diagnosis cannot be made based on clinical manifestations and chest X-ray examination alone

Method used

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  • Colloidal gold immunochromatography test paper for combined diagnosis of COVID-19 and mycoplasma pneumoniae and preparation method thereof
  • Colloidal gold immunochromatography test paper for combined diagnosis of COVID-19 and mycoplasma pneumoniae and preparation method thereof
  • Colloidal gold immunochromatography test paper for combined diagnosis of COVID-19 and mycoplasma pneumoniae and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The mouse anti-human IgM antibody concentration of the detection line IgM coating is 0.1mg / ml, and the mouse anti-human IgG antibody concentration of the detection line IgG coating is 0.1mg / ml; The quality control line C4 coating The goat anti-mouse IgG antibody concentration was 0.5mg / ml. The colloidal gold conjugate treatment pad 2 is coated with a new coronavirus N protein recombinant antigen-colloidal gold conjugate, and the diameter of the new coronavirus N protein recombinant antigen-colloidal gold conjugate is 40nm-100nm, and the new coronavirus recombinant antigen The concentration is 1 mg / ml.

[0057] The preparation method of the colloidal gold immunochromatography detection test paper of described joint diagnosis COVID-19 and Mycoplasma pneumoniae, described A detection test paper specifically comprises the following steps:

[0058] A) Preparation of test line T and quality control line C: Dilute the mouse anti-human IgM, mouse anti-human IgG and goat anti-m...

Embodiment 2

[0068] The difference from Example 1 is that the concentration of the mouse anti-human IgM antibody coated by the detection line IgM is 0.4 mg / ml, and the concentration of the mouse anti-human IgG antibody coated by the detection line IgG is 0.3 mg / ml; Quality control line C 4 coated goat anti-mouse IgG antibody concentration is 0.8mg / ml. The colloidal gold conjugate treatment pad 2 is coated with a new coronavirus N protein recombinant antigen-colloidal gold conjugate, and the diameter of the new coronavirus N protein recombinant antigen-colloidal gold conjugate is 40nm-100nm, and the new coronavirus recombinant antigen The concentration is 3 mg / ml.

[0069] The preparation method of the colloidal gold immunochromatography detection test paper of described joint diagnosis COVID-19 and Mycoplasma pneumoniae, described A detection test paper specifically comprises the following steps:

[0070] A) Preparation of test line T and quality control line C: Dilute mouse anti-human Ig...

Embodiment 3

[0080] The difference with Example 1 is that the concentration of the mouse anti-human IgM antibody coated by the detection line IgM is 1.0 mg / ml, and the concentration of the mouse anti-human IgG antibody coated by the detection line IgG is 1.0 mg / ml; The concentration of goat anti-mouse IgG antibody coated with quality control line C4 is 1.2mg / ml. The colloidal gold conjugate treatment pad 2 is coated with a new coronavirus N protein recombinant antigen-colloidal gold conjugate, and the diameter of the new coronavirus N protein recombinant antigen-colloidal gold conjugate is 40nm-100nm, and the new coronavirus recombinant antigen The concentration is 5mg / ml.

[0081] The preparation method of the colloidal gold immunochromatography detection test paper of described joint diagnosis COVID-19 and Mycoplasma pneumoniae, described A detection test paper specifically comprises the following steps:

[0082] A) Preparation of test line T and quality control line C: Dilute the mouse...

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Abstract

The invention relates to the technical field of immunodiagnosis, and discloses colloidal gold immunochromatography test paper for combined diagnosis of COVID-19 and mycoplasma pneumoniae aiming at theblank problem of COVID-19 and mycoplasma pneumoniae antibody detection in the prior art, which specifically comprises the following preparation steps: 1) preparing a detection line T and a quality control line C; 2) preparing a colloidal gold conjugate treatment pad; and 3) preparation of test paper: sequentially connecting and assembling the water absorption pad, the sample pad, the colloidal gold conjugate treatment pad, the antibody-coated modified nitrocellulose membrane and the PVC bottom plate to obtain a finished product. The prepared test paper can accurately detect whether a person has 2019-nCoV and mycoplasma pneumonia or not, is short in detection period, simple in detection process and portable in equipment, adopts an antibody antigen detection mode as the Antigen has no latent period, and the invention is visual and accurate in result display and high in specificity, can diagnose infection of new crowns and pulmonary branches at different time, and is high in detection efficiency.

Description

technical field [0001] The invention relates to the technical field of immunodiagnosis, in particular to a colloidal gold immunochromatographic test paper for combined diagnosis of COVID-19 and Mycoplasma pneumoniae. Background technique [0002] Acute respiratory diseases (ARD) account for a large proportion of all acute morbidity and mortality worldwide, with acute viral respiratory infections being the leading cause (approximately 80%). Major viral pathogens include influenza virus, respiratory disease syncytial virus (RSV), coronavirus, adenovirus and rhinovirus, and the combined global mortality rate of influenza and RSV alone reaches 300,000 deaths per year in children under 5 years of age . [0003] Novel coronavirus-infected pneumonia is an acute infectious pneumonia whose pathogen is a new type of coronavirus not previously found in humans, namely 2019-nCoV (novel coronavirus). The initial symptoms of patients are mostly fever, fatigue and dry cough, and gradually...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/56933G01N33/54313G01N33/558G01N2333/165G01N2333/30
Inventor 陈金树张芳陆维克高飞彭通李红玲吴晓诚
Owner HANGZHOU ALLTEST BIOTECH
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