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Lily symptomless virus and lily mottle virus composite colloidal gold immunochromatography detection reagent card and preparation method thereof

A lily mottle virus, immunochromatographic detection technology, applied in measuring devices, analytical materials, biological material analysis, etc., can solve problems such as difficult to meet virus detection requirements, and achieve rapid detection, easy operation, and high detection sensitivity.

Active Publication Date: 2014-10-29
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there are no relevant reports on simultaneous detection of LSV and LMoV by colloidal gold immunochromatography at home and abroad. LSV and LMoV composite colloidal gold immunochromatography detection reagent cards have not been developed, and it is difficult to meet domestic viewing and edible lily viruses. Testing needs

Method used

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  • Lily symptomless virus and lily mottle virus composite colloidal gold immunochromatography detection reagent card and preparation method thereof
  • Lily symptomless virus and lily mottle virus composite colloidal gold immunochromatography detection reagent card and preparation method thereof

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Embodiment Construction

[0022] like figure 1 and figure 2 The shown LSV and LMoV composite colloidal gold immunochromatographic detection reagent card includes a reagent card slot 1, a backing plate 11, a sample pad 7, a colloidal gold binding pad 8, a nitrocellulose membrane 9, and a water-absorbing filter paper 10, wherein the reagent card slot 1 includes an upper shell and a lower shell, the upper shell and the lower shell are connected by buckles, the upper shell is provided with a first hole 2 and a second hole 3, the sample pad 7 is placed under the first hole 2, and the nitrocellulose The plain film 9 is placed under the second hole 3, the colloidal gold binding pad 8 contains a composite gold standard probe, the liner 11 is fixed in the reagent card slot 1, the sample pad 7, the colloidal gold binding pad 8, and the nitrocellulose membrane 9 And water-absorbing filter paper 10 is arranged and connected in liner plate 11 upper surface successively, is provided with detection line 4,5 and con...

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Abstract

The invention discloses a lily symptomless virus and lily mottle virus composite colloidal gold immunochromatography detection reagent card and a preparation method thereof. The reagent card comprises a reagent card groove, a lining plate, a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and water-absorbing filter paper, wherein a composite gold mark probe is arranged on the colloidal gold combination pad; the lining plate is fixed in the reagent card groove; the sample pad, the colloidal gold combination pad, the nitrocellulose membrane and the water-absorbing filter paper are sequentially arranged and connected on the upper surface of the lining plate; the nitrocellulose membrane is provided with two detection lines and one control line; the two detection lines are respectively wrapped with anti-rabbit lily symptomless virus (LSV) and lily mottle virus (LMoV) polyclonal antibodies; the control line is wrapped with a goat anti-rabbit IgG purified antibody. The preparation method of the LSV and LMoV composite colloidal gold immunochromatography detection reagent card mainly comprises the following steps: respectively wrapping an immunochromatography membrane with the anti-rabbit LSV and LMoV polyclonal antibodies, and meanwhile preparing the colloidal gold combination pad. The reagent card can simultaneously detect LSV and LMoV, is high in accuracy rate, high in specificity and does not need special equipment or instrument.

Description

technical field [0001] The invention relates to a detection reagent card and a preparation method thereof, in particular to a reagent card for rapidly detecting lily recessive virus (LSV) and lily mottle virus (LMoV) by a composite colloidal gold immunochromatography method and a preparation method thereof. Background technique [0002] Lily (Lilium spp) is a world-renowned bulbous flower with a long history of cultivation, which integrates ornamental, edible and medicinal uses. As the largest flower planting country in the world, the Netherlands has planted 3800hm of lily bulbs by 2005. 2 , accounting for 72% of the total global area, produces 2.21 billion lily bulbs every year, of which about 100 million are exported to China. In China, the edible and medicinal lily industry has developed well, and the main production areas are in Gansu, Hunan and Hubei. For cut flower lilies, cut flower production began in my country in the late 1980s. However, due to the backwardness o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/558G01N33/56983G01N2333/08
Inventor 张玉宝王亚军谢忠奎郭志鸿王若愚何玉惠
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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