85results about How to "No need to dilute" patented technology

The invention relates to a kit for detecting brain natriuretic peptide in blood serum and provides the kit for detecting brain natriuretic peptide by nano microsphere immunonephelometry, which has the characteristics of no need of dilution of a sample, simple operation, high precision, good repeatability and suitability for a clinically common used automatic biochemical analyzer, in order to solve technical problems. The invention adopts a technical scheme that the kit for detecting the brain natriuretic peptide by nano microsphere immunonephelometry comprises the following components: a, a reagent R1 which comprises buffer solution, a preservative, a stabilizing agent, an electrolyte, a surfactant, and the balance of water; b, a reagent R2 which comprises buffer solution, nano microspheres combined with brain natriuretic peptide antibodies and a preservative, wherein the diameter of the microspheres is 50 to 150 nm; and c, a reference calibration material which comprises buffer solution, a stabilizing agent, a preservative, a recombinant brain natriuretic peptide pure product which is added in a corresponding amount according to the required concentration of the BNP (brain natriuretic peptide) reference calibration material, and the balance of water.

The invention relates to a chemiluminescence quantitative detection kit for procalcitonin, and a preparation method and detection method thereof. The kit comprises a procalcitonin series standard substance, a magnetic separation reagent (magnetic particle suspension coupled with streptavidin), a first reagent (anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label) and a second reagent (anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label). The sensitivity of the kit prepared from the magnetic particle suspension coupled with streptavidin, anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label and anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label is up to 0.008ng/ml; and the kit has the advantages of high accuracy, high precision, no need of prediluting the sample, and wide detection range, and is simple and time-saving to operate.

The invention relates to a centrifugal microfluidic serum separation chip and a preparation method thereof. The centrifugal microfluidic serum separation chip is a wafer-shaped microfluidic chip with a micro-structure and a micro-channel, and consists of a chip engraved with a micro-structure and a micro-channel and a blank chip. The centrifugal microfluidic serum separation chip is fixed on a rotation platform of a centrifugal machine, the separation operation of serum and erythrocyte in a micro-upgraded blood sample can be realized by the synergetic process of the micro-structure and the micro-channel on the chip, and the serum for analysis and detection can be acquired. The centrifugal microfluidic serum separation chip is applicable in related application fields, such as biomedicine, clinical diagnosis, health quarantine and the like.

The invention provides a method for distinguishing variety of fritillaria and detecting total alkaloid content of the fritillaria by virtue of near infrared spectrum. The method provided by the invention comprises the following steps: (1) collecting a fritillaria sample; (2) measuring the near infrared diffuse reflection spectrogram of the fritillaria sample, preprocessing the 4000-5000cm<-1> wave band in the spectrogram, and performing cluster analysis on the pre-processed near infrared spectrogram to build a qualitative model; or preprocessing the 4000-7000cm<-1> wave band in the spectrogram, so as to obtain an absorbance, associating the absorbance with the alkaloid content of the sample measured by virtue of bromothymol blue colorimetry, and building a quantitative correction model for detecting alkaloid by one or more methods of a partial least squares method, a principal component regression method and a multiple linear regression method; (3) collecting the near infrared spectrogram of the sample to be measured, after the corresponding preprocessing is performed, distinguishing the variety of the fritillaria and detecting total alkaloid content of the fritillaria by utilizing the built qualitative model or quantitative correction model. The method provided by the invention has the characteristics of fast speed, no damage, environment friendliness and low cost.

The invention relates to the medical field of immunoassay, particularly provides a measuring kit of the chemiluminescence immunoassay of a CA125 magnetic particle. The kit of the invention comprises: 1) a CA125 working calibrator; 2) the mixed solution of the magnetic particle coated with a CA125 monoclonal antibody and the CA125 monoclonal antibody marked by biotin; 3) streptavidin marked by horse radish peroxidase; 4) a chemiluminescence substrate; and 5) a reaction tube. Further, the method for preparing the kit according to the invention comprises the following steps: 1) the working calibrator is prepared by CA125 pure products; 2) the mixed solution of the magnetic particle coated with the monoclonal antibody and the monoclonal antibody marked by the biotin is prepared; 3) the horse radish peroxidase is used for marking the streptavidin; 4) the chemiluminescence substrate is prepared; 5) the working calibrator, the mixed solution, the enzyme markers, the chemiluminescence substrate and the reaction tube are sub-assembled; and 6) assembly is carried out to obtain a finished product. The kit of the invention has the advantages of simpleness and convenience, fastness, sensitivity and stability and the like.

The invention discloses a method for realizing a stable biological nitrosation to a wastewater with low carbon nitrogen ratio and high nitrogen content. The method controls the aging of system sludge to 3 days and combines the operational methods of constant water-feeding and interval draining together. The method is carried out according to the following two phases: 1. sludge seeding and incubation phase: firstly the seeded sludge is put into a reactor and the incubation time is 20 days, and an activated-sludge SBR nitrosation process combining constant water-feeding and interval-draining is adopted as the beginning of incubation; 2. stable biological nitrosation operational phase: the activated-sludge SBR nitrosation process combining constant water-feeding and interval-draining which is successfully started is preserved, and the wastewater with low carbon nitrogen ratio and high nitrogen content is adopted as water supply, and the feeding-draining flow each week is a half of the volume of the reactor, the system sludge aging is preserved to be 3 days, and the DO concentration of the reactor is 1.5 to 2mg per L, and the operational cycle is 12 hours. The invention realizes a stable and constant biological nitrosation to the wastewater with low carbon nitrogen ratio and high nitrogen content, and the nitrosation system has better NH4 <plus> minus N degradation rate and higher nitrosation rate.

The invention discloses a deep treatment method of high-concentration organic wastewater. The method comprises the following steps: step one, coagulating and precipitating; step two, carrying out ultraviolet-ozone-ultrasound combined reaction stage; step three, carrying out electrolysis treatment through three dimension electrodes; step four, recycling heavy metals; and step five, carrying out ultrafiltration-electrodialysis coupled desalinization. The deep treatment method of high-concentration organic wastewater is free of biological treatment processes, free of pretreatment, high in desalinization rate, simple to operate, high in efficiency, free of secondary pollution and low in cost, and is capable of completely removing organic matters, recycling the heavy metals and recycling the high-concentration organic wastewater.

The present invention provides 1, 3-propylene glycol preparing process, which includes the contact of dialkyl malonate with hydrogenating catalyst CuZnaAlbOc, where, a is 0.04-3, b is 0.04-3, and c is the atom number for reaching the electric neutrality of the compound; and reaction at temperature of 100-220 deg.c and pressure of 0.1-10.0 MPa, and in the space velocity of ester of 0.1-1/hr and hydrogen/ester molar ratio of 100-700 to 1. The process has high 1, 3-propylene glycol selectivity, high reactor utilizing efficiency, and low cost.

The invention discloses a method for processing paper-making waste water in the way of structuring artificial flora, including selecting more than two strains of Halomonas sp. flora and Bacillus sp. flora respectively for sterile culture to lead OD620nm to reach over 10.0 and then mixing the bacterial liquid by volume rate to structure the artificial flora; inoculating the paper-making waste water of pH being 9-11 and COD being 1,000-140,000mg l <-1> with the structured artificial flora, conducting stationary treatment for a certain time at 35-38 DEG C with intermittent stirring, in the treatment process, sampling at intervals to measure the pH, chroma and COD index of the paper-making waste water and finishing the treatment when the pH reduces to 7.0-8.8. The method is characterized by simple structure of the flora to be structured, obvious effect in direct treatment, strong controllability and repeatability and the like, and has great application prospect in the treatment aspects of waster water such as the control over the paper-making waste water.

The invention discloses a catalyst for preparing trichloroethylene from 1,1,1,2-tetrafluoroethane. The catalyst has the composition of wM/Al2OxFy, wherein the Al2OxFy is an active carrier; 2x+y=6; the y is greater than or equal to 1.8, but smaller than or equal to 4.2; the M is an active ingredient and is selected from one of Fe, Zr, La or Ce; the w is the mass ratio of the M to the Al2OxFy; the w is greater than or equal to 0.01, but smaller than or equal to 0.2. The preparation method of the catalyst comprises the following steps that a liquid phase fluorination method is used for preparing the active carrier Al2OxFy; then, an equivalent impregnation method is used for loading one of ferric nitrate, zirconium nitrate, lanthanum nitrate or cerium nitrate to form the catalyst. The catalyst has the advantages that the activity is high; the conversion rate of 1,1,1,2-tetrafluoroethane during the reaction at 410 DEG C can reach more than 60 percent; the service life of the catalyst is long; the stable operation for 300h can be realized; in addition, the introduction of diluent gas is not needed in the reaction process; industrial application values are realized.

The invention discloses a method for coating graphite on the surface of a rubber product by using a friction method, belonging to the field of surface treatment of the rubber product. The method for coating the graphite comprises the following steps of: (1) feeding: adding colloidal graphite for forging, pill particles and the rubber product into a drum-type mixer, wherein a ratio of the colloidal graphite for forging to the pill particles to the rubber product in volume is (0.5-2) to 2,000 to (1,000-2,000); (2) coating: rotating a rotary drum so that the colloidal graphite for forging is uniformly adhered to the surface of the rubber product; (3) drying: drying the rubber product coated with the colloidal graphite for forging at a room temperature or under a state of heating to a temperature which is equal to or less than 80 DEG C; and (4) polishing by friction: putting the dried rubber product and the dried pill particles into the dried rotary drum so as to brightly, uniformly and firmly adhere a graphite coating to the surface of the rubber product. According to the method disclosed by the invention, the rubber product has no need of being cleaned, the coating has no need of being cured, the adhering capability of the coating is strong, the graphite coating layer cannot release when the stretching formation of the rubber product reaches to 500%, and the material cost of a coating agent is low.

The invention relates to the technical field of medical products, and particularly discloses a compound stable chlorine dioxide solution prepared from the following raw materials by weight: 0.005% to 0.24% of a stable chlorine dioxide solution with a mass concentration of 2%, 0.01% to 0.1% of a hydrogen peroxide solution with a mass concentration of 27.5%, 0.1% to 0.26% of sodium carbonate, 0.1% to 0.4% of polyoxyethylene sorbitan monooleate, 0.005% to 0.01% of o-benzoyl sulfonic imide, 0.1% to 0.4% of peppermint oil, and the balance of water. Due to synergy of the raw materials, the compound stable chlorine dioxide solution can directly act on a human body without activation, is non-toxic and non-stimulating, and has a remarkable effect when being used for inflammation reduction and sterilization of human exposed organ inflammation and skin ucosal damage and operation wounds. A preparation method of the compound stable chlorine dioxide solution is simple and convenient to operate, easy to control, and suitable for industrial popularization and application.

The invention relates to an atomizing agent with arformoterol and glycopyrronium bromide serving as active components and a preparation method of the atomizing agent. The invention further relates to an application of glycopyrronium bromide in improving the stability of an arformoterol water solution. Arformoterol and glycopyrronium bromide can perform a synergistic function pharmacologically while glycopyrronium bromide is used for improving the stability of arformoterol, and accordingly, the local treatment function on the lung is improved.

The invention provides a novel isosorbide mononitrate injection composition containing 1,2-propylene glycol and phosphate buffer and a preparation method thereof. Phosphate buffer is used to adjust the pH of the injection to 6.8-7.9. The injection prepared by the application of the invention improves the purity of the effective components of the medicine, reduces the content of related substances, and reduces the toxicity at the same time.

The invention discloses a deep treatment method of high-salt organic sewage. The method comprises the following steps: step one, carrying out pretreatment; step two, carrying out electroflocculation and precipitation; step three, carrying out ultraviolet-ozone-ultrasound combined reaction stage; step four, carrying out electrolysis treatment through three dimension electrodes; step five, filteringthrough three-dimensional graphene sponge; and step six, carrying out ultrafiltration-electrodialysis coupled desalinization. The method is free of biological treatment processes and high in processflexibility; compared with the common sewage treatment method, the treatment period is greatly shortened; meanwhile, the method is high in desalinization rate, simple to operate, high in efficiency, free of secondary pollution and low in cost, and is capable of completely removing organic matters, recycling the heavy metals and directly recycling the treated high-salt organic sewage.

The invention discloses a method for acquiring a flanking sequence from genome DNA. The method comprises the following steps: 1) designing three specific primers having consistent directions and used for amplifying the flanking sequence according to a known sequence on the genome DNA, and sequentially marking the primers as GSP1, GSP2 and GSP3 according to the tapered distance between the primers and the flanking sequence; 2) performing first PCR (polymerase chain reaction) amplification by using the genome DNA as a template through a primer SAP (any of sequences 1-15) and the GSP1 to obtain a PCR product 1; 3) performing second PCR amplification by using the PCR product 1 as a template through primers SEP (sequences 16 and 17) and the GSP2 to obtain a PCR product 2; and 4) performing third PCR amplification by using the PCR product 2 as a template through a primer SNP (sequence 18) and the GSP3 to obtain a PCR product 3, thus acquiring the flanking sequence. The method has the following advantages of simplicity in operation, high specific amplification, high success rate, short time and the like; and large fragments are easily obtained.

The invention discloses an indigo carmine colorant as well as a preparation method and applications thereof. A mixed solution of indigo carmine and antioxidants contains 0.1%-1%(weight/volume percent concentration, g/100ml) of indigo carmine, the antioxidants are selected from one or combinations of ascorbic acid, tea polyphenols and phytic acid, and the amount of addition is 0.05%-0.5%(weight/volume percent concentration, g/100ml). The indigo carmine colorant has advantages as follows: a product is greatly improved in stability, is easy to use and store, and has a good clinical effect; the safety performance is high, and the clinical risk is low; the operation is simple. A prepared indigo carmine solution can be directly used in clinical treatment, dilution and other additional operations are not required, a clinical operation process is greatly simplified, and defects of a colorant obtained with an immediate preparation and use method in the prior art are solved.

The invention discloses a novel atomized inhalation solution preparation of heparin pharmaceutically acceptable salt and derivative thereof and a preparation method thereof; a liquid preparation for atomizing inhalation comprises a pharmaceutically acceptable salt of heparin, a surfactant, an isotonic agent, an appropriate amount of a PH modulator and purify water, and can be used for treating lung diseases, in particular chronic obstructive pulmonary disease (COPD), acute lung injury and acute respiratory distress syndrome.

The invention belongs to the technical field of marine environment monitoring and relates to an online monitoring and in-situ monitoring device and method for total nitrogen/total phosphorus of seawater. The detection device comprises a first flowing channel and a second flowing channel which are connected in sequence; the first flowing channel or the second flowing channel comprises a base, a light splitting assembly, LED light sources, a photodiode and a flowing pond; the light splitting assemblies and the flowing ponds are embedded in the bases; light through holes are formed between the light splitting assemblies and the flowing ponds; the LED light sources are optically connected with the light splitting assemblies; one photodiode is vertical to the LED light sources; the other photodiode is horizontal to the LED light sources; the optical length of the flowing pond of the first flowing channel is greater than that of the flowing pond of the second flowing channel. According to the device and method, the influence of light source fluctuation caused by other factors on the defection result can be eliminated, high sample concentration or low sample concentration can be measuredat a time, and the device and method have the advantages that a high-concentration sample does not need to be diluted, the structure is relatively compact, and the reference light intensity can be adjusted.