Method for treating papermaking waste water by constructing artificial flora

A technology of papermaking wastewater and bacterial colony, which is applied in the direction of processing wastewater treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as difficult to repeat and control, unclear treatment process, uncontrollability, etc.

Inactive Publication Date: 2009-04-15
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In a word, the above reports and patents did not elaborate and study the microbial treatment process conditions and treatment mechanism when using bacteria to treat papermaking wastewater, so there are short

Method used

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  • Method for treating papermaking waste water by constructing artificial flora
  • Method for treating papermaking waste water by constructing artificial flora
  • Method for treating papermaking waste water by constructing artificial flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: The determination of each bacterial strain's growth demand for oxygen, temperature tolerance, pH and salinity tolerance

[0046](1) Strain selection: Four strains of Halomonas (Halomonas) isolated from various extreme natural environments such as strong alkali and high salinity and preserved in the "China Typical Culture Collection Center" were selected on October 29, 2008. ) sp.Y2 CCTCC NO: M 208188, Halomonas (Halomonas) sp.17-5 CCTCC NO: M 208192, Halomonas (Halomonas) sp.19-A CCTCC NO: M 208186 or Halomonas ( Halomonas) sp.19-D CCTCC NO: M 208193 and seven strains of Bacillus (Bacillus) sp.Y4 CCTCC NO: M 208189, Bacillus (Bacillus) sp.Y5CCTCC NO: M 208190, Bacillus (Bacillus) sp.17 -1 CCTCC NO: M 208183, Bacillus sp.17-3 CCTCC NO: M 208184, Bacillus sp.17-4 CCTCC NO: M 208185, Bacillus sp.19-B CCTCC NO: M 208187 or Bacillus sp.Y6 CCTCC NO: M 208191;

[0047] First, select the strains in the above-mentioned Halomonas sp.Y2, Halomonas sp.17-5, Halomona...

Embodiment 2

[0063] Embodiment 2: the method for constructing artificial flora to treat papermaking wastewater

[0064] Select Halomonas (Halomonas) sp.Y2CCTCC NO: M 208188 and Halomonas (Halomonas) sp.19-A CCTCC NO: M 208186 strains to carry out the following operations respectively:

[0065] (1) Slant culture: the above-mentioned Halomonas strain was inoculated on a solid slant medium containing 1.6% agar powder by mass percentage and a pH of 8.0, and the cells were cultured at 30° C. for 20 hours;

[0066] (2) First-level seed cultivation: the thallus cultivated in step (1) was put into 100ml complete liquid medium with an inoculation loop under aseptic conditions, and cultured on a shaker at 200 rpm for 16 hours at 30°C. Hours to the middle of the logarithmic growth phase, the first-class seeds were obtained;

[0067] (3) Expansion cultivation: with the inoculum size of 5% by volume ratio, connect the primary seeds in 1000ml complete liquid medium, shake and cultivate for 16 hours on ...

Embodiment 3

[0078] Embodiment 3: the method for constructing flora to carry out papermaking wastewater treatment

[0079] Select Halomonas sp.Y2 CCTCC NO: M 208188, Halomonas sp.17-5 CCTCC NO: M 208192 and Halomonas sp.19-A CCTCC NO: The M 208186 strain was subjected to the following operations:

[0080] (1) Slant culture: the above-mentioned Halomonas strain was inoculated on a solid slant medium containing 1.6% agar powder by mass percentage and a pH of 8.0, and the cells were cultured at 30° C. for 20 hours;

[0081] (2) First-level seed cultivation: the thallus cultivated in step (1) was put into 100ml complete liquid medium with an inoculation loop under aseptic conditions, and cultured on a shaker at 200 rpm for 16 hours at 30°C. Hours to the middle of the logarithmic growth phase, the first-class seeds were obtained;

[0082] (3) Expansion cultivation: with the inoculum size of 5% by volume ratio, connect the primary seeds in 1000ml complete liquid medium, shake and cultivate for...

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Abstract

The invention discloses a method for processing paper-making waste water in the way of structuring artificial flora, including selecting more than two strains of Halomonas sp. flora and Bacillus sp. flora respectively for sterile culture to lead OD620nm to reach over 10.0 and then mixing the bacterial liquid by volume rate to structure the artificial flora; inoculating the paper-making waste water of pH being 9-11 and COD being 1,000-140,000mg l <-1> with the structured artificial flora, conducting stationary treatment for a certain time at 35-38 DEG C with intermittent stirring, in the treatment process, sampling at intervals to measure the pH, chroma and COD index of the paper-making waste water and finishing the treatment when the pH reduces to 7.0-8.8. The method is characterized by simple structure of the flora to be structured, obvious effect in direct treatment, strong controllability and repeatability and the like, and has great application prospect in the treatment aspects of waster water such as the control over the paper-making waste water.

Description

technical field [0001] The invention relates to a method for treating waste water by means of flora, in particular to a method for treating papermaking waste water by constructing artificial flora and obtaining a process route of a two-step treatment mechanism. Background technique [0002] At present, the demand for paper in the world is showing a sharp upward trend. In developed countries, because the pulp raw materials are mainly wood, a large number of forests and wood are gradually decreasing. The lack of wood raw material resources makes straw pulp papermaking very common in developing countries. Straw pulp papermaking derived from the alkaline pulping process has the advantages of good paper quality and cheap and abundant raw materials. But as we all know, the biggest disadvantage of straw pulp papermaking is the serious pollution to the environment due to the high pollution load. [0003] Different from wood pulp wastewater, straw pulp papermaking wastewater has the...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F103/28
Inventor 许平杨春玉王照华马翠卿苏海军李阳
Owner SHANDONG UNIV
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