25 results about "Halomonas sp." patented technology

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.

This invention provides a method for preparing a high-salinity zinc oxide-resistant nanofluid mediated by Halomonas sp. and its application in enhancing oil recovery. Specifically, it includes: a Halomonas sp. strain CDH-2, with accession number CCTCC NO: M 2025786, which can rapidly grow in high-concentration salt solutions and produce salt-resistant extracellular metabolites. Fermenting and culturing CDH-2 under high-salt conditions yields a fermentation broth with a surface tension below 30 mN / m. Mixing and culturing the obtained fermentation broth with a metal precursor solution yields a high-salinity-resistant metal nanofluid. The resulting metal nanofluid maintains good stable dispersibility in high-salinity water and effectively reduces oil-water interfacial tension. It alters the wettability of oil-wetting surfaces, emulsifies and disperses crude oil, and reduces crude oil viscosity. Therefore, it can be used as an enhanced oil recovery agent in high-salt reservoirs and is widely applied in oilfield flooding, well huff and puff, and heavy oil viscosity reduction.

The present invention relates to a recombinant halomonas comprising an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), whereby the copolymer can be synthesized from a xylose-containing cellulose hydrolysate, the LA ratio can be controlled by further promoter modification and process optimization, and industrial enlargement is facilitated.

The present invention relates to a novel Halomonas lithopenaei strain having good polyhydroxyalkanoate productivity under high salt conditions, and use thereof, a novel Halomonas lithopenaei YBW-3-4-1 strain having been selected, from among Halomonas sp. strains differing as to whether polyhydroxyalkanoate is produced, that produces polyhydroxyalkanoate at notably high volume and concentration under high salt conditions, the strain can be used to produce polyhydroxyalkanoate.

The application provides halomonas and application thereof in preparation of PDRN and related products, and relates to the technical field of biotechnology. Halomonas sp. The application extracts natural PDRN from halomonas for the first time, and provides a new way for production of PDRN. The application further provides a method for extracting natural PDRN from halomonas, which is safe and controllable in the whole closed-loop management from culture of the strain to fermentation, and simple in preparation method, free of extreme reaction conditions and easy for large-scale industrial production.

The application discloses a coal chemical high-salt high-phenol wastewater treatment method and system, and the method comprises the following steps: detoxification pretreatment, salt-tolerant biochemical treatment, deep desalination and crystallization, and resource recovery. The application adopts a process route of "front-end deep detoxification and impurity removal + middle-end salt-tolerant biochemical synergy". First, through the precise pretreatment of "ammonia evaporation and ammonia removal" and "extraction and phenol extraction", ammonia nitrogen and phenols and other toxic substances are efficiently removed at the source, and the inhibiting factors of the subsequent biochemical unit are fundamentally removed. Then, combined with the domesticated salt-tolerant microbial flora (Halomonas and Chromohalobacter), the biochemical system can stably operate in a high-salt environment with TDS>15000 mg / L, and the COD removal rate is stably above 80%, so that the combined inhibition problem of high salt, high phenol and high ammonia nitrogen to biochemical treatment is solved, and the long-term stable operation of the whole process is ensured.

The present application provides a kind of marine bacteria capable of degrading lignin and removing nitrite, which is a deep-sea seabed sulfide source, and has the function of degrading lignin and removing nitrite.The strain of Halomonas sp.has a preservation number of CGMCC No.34690.The strain screened in the present application can grow and reproduce in seawater, can utilize guaiacol as a carbon source, and can remove nitrite.The strain of the present application has laccase activity, has laccase gene and nitrite reductase gene.The experimental results show that the cumulative removal rate of guaiacol after 2 days of culture is 56%, and the removal rate of nitrite after 4 days of culture is close to 70%, which has great application potential in the marine aquaculture industry.

The invention belongs to the technical field of microorganisms, and particularly relates to halophilic bacteria for high-yield biomacromolecular materials and high-efficiency expression application of the halophilic bacteria. The invention provides a halophilic bacterium PYL-X1 with high protein yield, the halophilic bacterium PYL-X1 is classified and named as halophilic bacteria (Halophiles) and is preserved in Guangdong Microbial Culture Collection Center on May 6, 2025, and the preservation number is GDMCC No: 66258; the halophilic bacteria PYL-X1 with high protein yield can efficiently produce various recombinant proteins after being introduced into a recombinant protein expression plasmid system, the shake flask level yield is as high as 210 mg / L-300 mg / L, the yield of recombinant fibronectin in a 5L fermentation tank is as high as 7.3 g / L, a new microbial resource is provided for efficient preparation of recombinant proteins, and the halophilic bacteria PYL-X1 has a better industrial application prospect.

The invention relates to an adaptive evolution culture method of Halomonas and application of the Halomonas in preparation of PHB, and belongs to the technical field of biology. According to the method, the red algae are pretreated with the acid with the specific concentration, the hydrolysis rate of the red algae can be increased, and more raw materials are provided for PHB preparation. According to the invention, the halomonas is subjected to 35 times of adaptive evolution culture in the red algae pre-hydrolysate, so that the tolerance and transformation capability of the strain to an inhibitor are improved, the prepared culture solution containing the halomonas evolved strain is used for preparing PHB (poly-3-hydroxybutyrate), and the yield of the PHB can be obviously improved. According to the method, the PHB is prepared by fermentation of the Halomonas evolved strain, the red algae are taken as the raw material, the hydrolysate obtained by pre-hydrolysis does not need solid-liquid separation, and fermentation is performed after enzymolysis of cellulase, so that more raw materials can be released, the content of the PHB is further increased, the utilization rate of the red algae is increased, and meanwhile, the preparation process steps are simplified.

The application belongs to the technical field of genetic engineering, and particularly relates to a novel EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) mutant with high glyphosate tolerance, and further discloses application of a coding gene thereof in the field of novel glyphosate-tolerant crop cultivation. A 5-enolpyruvylshikimate-3-phosphate synthase is cloned from a marine strain Halomonas sp., and then through three rounds of directed evolution technology, multiple mutants with significantly improved glyphosate tolerance are obtained. The mutant coding gene is transferred into tobacco, and the tobacco is endowed with high resistance to herbicides, which proves that the mutant has high application value in the cultivation of herbicide-tolerant plants.

This invention relates to the application of a highly efficient halophilic denitrifying bacterium of the genus *Halomonas*. The strain is *Halomonas ramblicola* 5505, a salt-tolerant heterotrophic nitrifying-aerobic denitrifying bacterium, with accession number CGMCC No. 30683. The strain primarily removes ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen from water through assimilation, achieving assimilation rates of 62.68%, 51.08%, and 52.66%, respectively; it can also dissimilate some inorganic nitrogen into nitrogen-containing gases. In simulated nitrogen-containing wastewater, the strain achieved a removal rate of over 90% for ammonia nitrogen and nitrate nitrogen within 12 hours, and increasing the carbon-to-nitrogen ratio completely removed accumulated nitrite nitrogen. This strain effectively solves the problem of inhibition of biological denitrification microorganisms by high-salt nitrogen-containing wastewater. Its nitrogen removal process is green, efficient, and energy-saving, and can be applied to the ecological treatment and restoration of water bodies polluted by high salt and nitrogen.

The application provides a biological organic fertilizer suitable for saline-alkali soil and a preparation method and application thereof, and belongs to the field of microbial fertilizers.The biological organic fertilizer is prepared from halomonas sp ZH-1, including organic acid fermentation liquor, perlite, activated carbon, distilled water, halomonas sp, pancreatin and yeast extract.The biological organic fertilizer can convert insoluble phosphate into soluble phosphate which can be absorbed by plants, can synthesize indole acetic acid by using tryptophan, and has the ability of synthesizing iron carrier.The biological organic fertilizer can improve the chemotaxis distance of halomonas sp, enhance the activity of halomonas sp in soil, improve the colonization ability of the strain in the rhizosphere, improve the stress resistance of wheat under salt stress, promote the growth of wheat, improve the thousand-grain weight of wheat, and finally improve the yield of wheat.

The application provides a recombinant bacterium for producing 3-hydroxypropionic acid, and a construction method and application thereof. The recombinant bacterium expresses a halomonas sp. derived accA gene, accB gene and malonyl-CoA reductase. The application fully utilizes the 3HB synthesis pathway of the strain itself, cooperates with the introduced 3-HP synthesis path, realizes high-efficiency synthesis of poly(3-hydroxybutyric acid-3-hydroxypropionic acid) or poly(3,4-dihydroxybutyric acid-3-hydroxypropionic acid) without passing through the intermediate product 3-hydroxypropionic acid aldehyde, and avoids the toxic effect of the intermediate product on cells.

The application belongs to the technical field of microorganisms, and discloses a halophilic halomonas and application thereof. Franzmannia qiaohouensis The halomonas is separated from the millet rhizosphere soil of saline-alkali land, has a strain number STM-122, a preservation number CGMCC No. 36349, is identified and classified by 16S rRNA gene sequence identification and phylogenetic analysis, and has the functions of phosphorus solubilization, EPS production, auxin production and iron carrier production. Through seed treatment by bacterial suspension, the germination energy and germination rate of millet seeds under saline-alkali stress can be significantly improved, and the indexes such as seedling root length and plant height are promoted; meanwhile, a complete preparation method of the fermentation bacterial fertilizer of the strain is provided, and the obtained fermentation bacterial fertilizer has a viable bacterial count ≥1×10 8 CFU / g. The application can enrich the salt-tolerant microbial resources, and provide technical support for the planting of millet and other crops in saline-alkali land.

The application provides an organic carrier endo- rhizosphere growth promoting microbial agent for degraded grassland ecological restoration and a preparation method and application thereof, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth promoting microbial agent comprises four endo- rhizosphere growth promoting bacteria (PGPE), four rhizosphere growth promoting bacteria (PGPR) and an organic carrier; the PGPE comprises B. terrigena K4, B. subtilis 9-3-1, B. safensis 11-5-4 and Halomonas sp. L31, the PGPR comprises Serratia marcescens 5, Serratia marcescens 23, Kosakonia koreensis 11 and Enterobacter cloacae 24, and the organic carrier is carbon-based organic fertilizer, cattle and sheep manure organic fertilizer or biochar. The application of the organic carrier growth promoting microbial agent in degraded grassland can significantly improve the biomass and coverage of the plant community. The organic carrier growth promoting microbial agent can be used for the ecological restoration of degraded grassland.

The invention relates to the technical field of biological materials, in particular to engineering modified halomonas, a construction method thereof and a method for producing a tissue filling material by using the engineering modified halomonas. The engineering modified halomonas is obtained by overexpressing a phaAB gene (SEQ ID NO: 2) through a promoter porin 59 (SEQ ID NO: 1) in the Halomonas blue phaseensis CYL37, and the engineering modified halomonas is obtained by overexpressing the phaAB gene (SEQ ID NO: 2) through the promoter porin 59 (SEQ ID NO: 1). The material for tissue filling prepared by the embodiment of the invention has better mechanical properties, and can realize the effects of long-acting filling and skin improvement.

The application discloses a heterotrophic nitrification and aerobic denitrification bacteria for treating high-salt and high-ammonia-nitrogen wastewater, and relates to a high-salt and high-ammonia-nitrogen wastewater treatment method. Halomonas sp The bacteria strain is Halomonas sp. Halomonas , named MY-11, belongs to the genus Halomonas, is preserved in the China Center for Type Culture Collection, has a preservation number of CCTCC M 20242923 and a preservation date of December 27, 2024. The bacteria strain is a heterotrophic nitrification and aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water bodies, has good denitrification performance under high-salt and high-ammonia-nitrogen stress, and has high tolerance to high-salt and high-ammonia-nitrogen. The bacteria strain can efficiently treat nitrogen-containing wastewater under the conditions of 2%-6% salinity and 100 mg / L-400 mg / L ammonia-nitrogen concentration, and the removal efficiency can be higher than 99%. The bacteria strain has great potential in the application of inorganic nitrogen pollution treatment of various water bodies such as breeding and chemical industry wastewater.

The application discloses a strain of Lactobacillus plantarum NX3-5 and application thereof, and belongs to the technical field of beneficial bacteria. Halomonas sp. The application discloses a strain of Lactobacillus plantarum NX3-5 and application thereof, and belongs to the technical field of beneficial bacteria. Halomonas sp. The application discloses a strain of Lactobacillus plantarum NX3-5 and application thereof, and belongs to the technical field of beneficial bacteria. The application provides the strain of Lactobacillus plantarum NX3-5, which is preserved in the China Center for Type Culture Collection on December 18, 2025, and has a preservation number of CCTCC M 20252959; the provided strain has high salt and alkali tolerance; has the ability of reducing salt and alkali, and the alkali reduction rate is 18.22 %; further has the abilities of nitrogen fixation, potassium solution, organic phosphorus dissolution, protease production and IAA production, and can significantly promote the growth of the aboveground and underground parts of cucumber plants under normal conditions and salt and alkali stress conditions.

The application provides an engineered halomonas and a method for producing acetoin, wherein the halomonas expresses acetyl-lactate synthase and acetyl-lactate decarboxylase, and does not express PHA hydratase and acetoacetyl-CoA or the expressed PHA hydratase and acetoacetyl-CoA are not functional or have reduced function. The engineered halomonas can realize economic, sustainable and efficient production of acetoin, and improve single batch fermentation yield.

This invention belongs to the field of biological wastewater treatment, specifically relating to a composite microbial agent, its preparation method, and its application. By mass percentage, it comprises 60%-80% bioactive agent and 20%-40% activity protectant. The bioactive agent includes 66.58%-86.58% of *Haloxylon ammodendron* strains, 7.85%-22.85% of *Hymenobacter* strains, and 5.57%-25.57% of *Thiobacillus* strains. The total viable count of the composite microbial agent is not less than 1 × 10⁻⁶. 9 The compound microbial agent was added to distilled water and cultured under anaerobic shaking conditions to obtain a revived bacterial solution. The supernatant of the revived bacterial solution was mixed with the wastewater to be treated at a volume ratio of 1-10:250, and 100-250 mg / L of a compatible solute was added. The wastewater was then treated under anaerobic conditions. After one week of treatment of low C / N ratio, high-salt phenol wastewater using this compound microbial agent, the denitrification rate remained above 95%, and the phenol degradation rate remained above 80%. This effectively solved the problems of low denitrification efficiency and difficulty in removing phenolic compounds from high-salt phenol wastewater, and the process was simple and low-cost.

The invention provides halomonas and application thereof in preparation of PDRN and related products, and relates to the technical field of biology. According to the method disclosed by the invention, the natural PDRN is extracted from the Halomonas sp. For the first time, and a new way is provided for the production of the PDRN. The invention further provides a method for extracting the natural PDRN from the halomonas, closed-loop management is achieved in the whole process from strain culture to tank fermentation, safety and controllability are achieved, and the preparation method is simple, free of extreme reaction conditions and easy for large-scale industrial production.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a low-carbon-nitrogen-ratio salt-tolerant efficient compound nitrogen removal flora as well as a construction method and application thereof. The invention discloses a low-carbon-nitrogen-ratio salt-tolerant high-efficiency compound nitrogen removal flora which comprises acinetobacter hemolyticus ZJB21135 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M20211543; and / or halomonas W06, and the preservation number of the halomonas W06 is CCTCC (China Center For Type Culture Collection) NO: M20242131. The screened and cultured mixed bacteria integrate salt-resistant and high-efficiency compound nitrogen removal performance, the optimal compound nitrogen removal effect can be achieved by optimizing culture conditions, the optimal compound nitrogen removal effect is 100% and 88.18%, meanwhile, the flora has a very good removal effect on ammonia nitrogen and nitrate nitrogen, and the compound nitrogen removal efficiency is greatly improved. The method has important practical significance for developing an efficient denitrification biological agent and reducing the treatment cost of microbial denitrification.

This invention discloses an engineered strain of *Haloxymonas* for biological oxidative desulfurization and its whole-cell catalytic desulfurization method. The engineered strain is a recombinant *Haloxymonas* containing a quinone oxidoreductase gene. The recombinant *Haloxymonas* expresses the quinone oxidoreductase. The recombinant *Haloxymonas* of this invention achieves a maximum biological hydrogen sulfide removal rate of 80.216 μmol·min under alkaline conditions. ‑1 ·g ‑1 The cell dry weight showed a highly efficient hydrogen sulfide removal rate; it can be recycled 6 times in a whole-cell catalytic desulfurization system, and the biological desulfurization rate is maintained at 40 μmol·min⁻¹. ‑1 ·g ‑1 Above the cell dry weight, it exhibited good stability, with an average rate reaching 56.843 μmol·min. ‑1 ·g ‑1 Cell dry weight. Therefore, the above-mentioned recombinant halometabolite and its whole-cell biological desulfurization process have application value in the field of biological removal of hydrogen sulfide.

This invention relates to the field of microbial metabolic engineering, and particularly to a recombinant *Halomonas* strain that synthesizes P3HB3HP from glucose, its construction method, and its applications. This invention constructs a synthetic pathway in *Halomonas* containing enzymes such as aspartate decarboxylase and 4-aminobutyrate aminotransferase. The resulting recombinant *Halomonas* strain can synthesize P3HB3HP with a high 3HP ratio from glucose alone via the β-alanine pathway, while maintaining a high P3HB3HP yield. The recombinant *Halomonas* strain provided by this invention can produce P3HB3HP using inexpensive carbon sources, significantly reducing substrate costs and achieving high cell density, high yield, high 3HP ratio, and stable P3HB3HP synthesis.