44 results about "Halomonas sp." patented technology

The invention provides a special charcoal-based slow-release fertilizer for sandy soil as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The special charcoal-based slow-release fertilizer for the sandy soil is prepared from amino modified biomass charcoal, urea, urea formaldehyde, monoammonium phosphate, potassium sulfate, polyglutamic acid, fulvic acid, clay, attapulgite, a compound microbial agent and sodium alginate. The compound microbial agent is prepared from halomonas (ACCC 11681), bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094), and the compound microbial agent is prepared from bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094). The invention further provides a preparation method and application of the special charcoal-based slow-release fertilizer for the sandy soil. The special charcoal-based slow-release fertilizer for the sandy soil can remarkably improve the water and fertilizer retention capacity of the sandy soil, improve the farmland productivity of the sandy soil, save fertilizer and water input and improve the yield and quality of crops.

The invention discloses a heterotrophic nitrification and aerobic denitrification halomonas sp. ZC-1 with low temperature resistance and high salt, and the preservation number of the heterotrophic nitrification and aerobic denitrification halomonas sp. ZC-1 is CCTCC (China Center for Type Culture Collection) M 20241695. The strain ZC-1 screened by the invention can simultaneously tolerate the influence of low temperature and high salt, and still can efficiently remove ammonia nitrogen under double stress conditions, especially under the environment of the temperature of 10 DEG C and the salinity of 10% w / v, the ammonia nitrogen removal rate reaches 94% or above, the TN removal rate reaches 93% or above, and salt-containing wastewater in northern China in winter can be treated in a targeted manner.

The invention provides a Halomonas sp. Strain with saline-alkaline tolerance, the Halomonas sp. Strain is named as Vreelandella sp. Strain, is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.30231, the preservation date is April 1, 2024, and the 16S rRNA nucleotide sequence is as shown in SEQ ID NO: 1. The invention further provides a preparation method of the Halomonas sp. Strain. The invention further provides preparation of a microbial fertilizer which can be used for improving saline-alkali resistance of plants and promoting plant growth and can also be used for promoting plant growth and improving saline-alkali soil. The strain can relieve damage of saline-alkali stress to plants and promote plant growth, can be used for preparing functional fertilizers for soil improvement and plant growth promotion, and has wide application prospects in the aspects of saline-alkali soil improvement, agricultural productivity improvement, agricultural ecological environment protection and the like.

The invention discloses halomonas for efficiently synthesizing nano-selenium as well as a method for preparing the nano-selenium and application of the halomonas, and belongs to the technical field of biology. The Halomonas sp. CCTCC NO: M 20242266 disclosed by the invention has strong tolerance to high-concentration selenite, can efficiently convert the selenite into nano-selenium, and is simple in required culture condition, low in cost and easy to operate; the nano-selenium particles produced by utilizing the strain disclosed by the invention are stable in property and high in biological activity. The Halomonas sp. CCTCC NO: M 20242266 or a microbial agent of the Halomonas sp. CCTCC NO: M 20242266 or nano-selenium produced by utilizing the strain has wide development prospects in the fields of functional agriculture including planting industry and breeding industry, functional food processing, health food processing, medical product processing and the like.

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.

This invention provides a method for preparing a high-salinity zinc oxide-resistant nanofluid mediated by Halomonas sp. and its application in enhancing oil recovery. Specifically, it includes: a Halomonas sp. strain CDH-2, with accession number CCTCC NO: M 2025786, which can rapidly grow in high-concentration salt solutions and produce salt-resistant extracellular metabolites. Fermenting and culturing CDH-2 under high-salt conditions yields a fermentation broth with a surface tension below 30 mN / m. Mixing and culturing the obtained fermentation broth with a metal precursor solution yields a high-salinity-resistant metal nanofluid. The resulting metal nanofluid maintains good stable dispersibility in high-salinity water and effectively reduces oil-water interfacial tension. It alters the wettability of oil-wetting surfaces, emulsifies and disperses crude oil, and reduces crude oil viscosity. Therefore, it can be used as an enhanced oil recovery agent in high-salt reservoirs and is widely applied in oilfield flooding, well huff and puff, and heavy oil viscosity reduction.

The present invention relates to a recombinant halomonas comprising an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), whereby the copolymer can be synthesized from a xylose-containing cellulose hydrolysate, the LA ratio can be controlled by further promoter modification and process optimization, and industrial enlargement is facilitated.

The present invention relates to a novel Halomonas lithopenaei strain having good polyhydroxyalkanoate productivity under high salt conditions, and use thereof, a novel Halomonas lithopenaei YBW-3-4-1 strain having been selected, from among Halomonas sp. strains differing as to whether polyhydroxyalkanoate is produced, that produces polyhydroxyalkanoate at notably high volume and concentration under high salt conditions, the strain can be used to produce polyhydroxyalkanoate.

The application provides halomonas and application thereof in preparation of PDRN and related products, and relates to the technical field of biotechnology. Halomonas sp. The application extracts natural PDRN from halomonas for the first time, and provides a new way for production of PDRN. The application further provides a method for extracting natural PDRN from halomonas, which is safe and controllable in the whole closed-loop management from culture of the strain to fermentation, and simple in preparation method, free of extreme reaction conditions and easy for large-scale industrial production.

The application discloses a coal chemical high-salt high-phenol wastewater treatment method and system, and the method comprises the following steps: detoxification pretreatment, salt-tolerant biochemical treatment, deep desalination and crystallization, and resource recovery. The application adopts a process route of "front-end deep detoxification and impurity removal + middle-end salt-tolerant biochemical synergy". First, through the precise pretreatment of "ammonia evaporation and ammonia removal" and "extraction and phenol extraction", ammonia nitrogen and phenols and other toxic substances are efficiently removed at the source, and the inhibiting factors of the subsequent biochemical unit are fundamentally removed. Then, combined with the domesticated salt-tolerant microbial flora (Halomonas and Chromohalobacter), the biochemical system can stably operate in a high-salt environment with TDS>15000 mg / L, and the COD removal rate is stably above 80%, so that the combined inhibition problem of high salt, high phenol and high ammonia nitrogen to biochemical treatment is solved, and the long-term stable operation of the whole process is ensured.

The present application provides a kind of marine bacteria capable of degrading lignin and removing nitrite, which is a deep-sea seabed sulfide source, and has the function of degrading lignin and removing nitrite.The strain of Halomonas sp.has a preservation number of CGMCC No.34690.The strain screened in the present application can grow and reproduce in seawater, can utilize guaiacol as a carbon source, and can remove nitrite.The strain of the present application has laccase activity, has laccase gene and nitrite reductase gene.The experimental results show that the cumulative removal rate of guaiacol after 2 days of culture is 56%, and the removal rate of nitrite after 4 days of culture is close to 70%, which has great application potential in the marine aquaculture industry.

The invention belongs to the technical field of microorganisms, and particularly relates to halophilic bacteria for high-yield biomacromolecular materials and high-efficiency expression application of the halophilic bacteria. The invention provides a halophilic bacterium PYL-X1 with high protein yield, the halophilic bacterium PYL-X1 is classified and named as halophilic bacteria (Halophiles) and is preserved in Guangdong Microbial Culture Collection Center on May 6, 2025, and the preservation number is GDMCC No: 66258; the halophilic bacteria PYL-X1 with high protein yield can efficiently produce various recombinant proteins after being introduced into a recombinant protein expression plasmid system, the shake flask level yield is as high as 210 mg / L-300 mg / L, the yield of recombinant fibronectin in a 5L fermentation tank is as high as 7.3 g / L, a new microbial resource is provided for efficient preparation of recombinant proteins, and the halophilic bacteria PYL-X1 has a better industrial application prospect.

The invention relates to an adaptive evolution culture method of Halomonas and application of the Halomonas in preparation of PHB, and belongs to the technical field of biology. According to the method, the red algae are pretreated with the acid with the specific concentration, the hydrolysis rate of the red algae can be increased, and more raw materials are provided for PHB preparation. According to the invention, the halomonas is subjected to 35 times of adaptive evolution culture in the red algae pre-hydrolysate, so that the tolerance and transformation capability of the strain to an inhibitor are improved, the prepared culture solution containing the halomonas evolved strain is used for preparing PHB (poly-3-hydroxybutyrate), and the yield of the PHB can be obviously improved. According to the method, the PHB is prepared by fermentation of the Halomonas evolved strain, the red algae are taken as the raw material, the hydrolysate obtained by pre-hydrolysis does not need solid-liquid separation, and fermentation is performed after enzymolysis of cellulase, so that more raw materials can be released, the content of the PHB is further increased, the utilization rate of the red algae is increased, and meanwhile, the preparation process steps are simplified.

The application belongs to the technical field of genetic engineering, and particularly relates to a novel EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) mutant with high glyphosate tolerance, and further discloses application of a coding gene thereof in the field of novel glyphosate-tolerant crop cultivation. A 5-enolpyruvylshikimate-3-phosphate synthase is cloned from a marine strain Halomonas sp., and then through three rounds of directed evolution technology, multiple mutants with significantly improved glyphosate tolerance are obtained. The mutant coding gene is transferred into tobacco, and the tobacco is endowed with high resistance to herbicides, which proves that the mutant has high application value in the cultivation of herbicide-tolerant plants.

This invention relates to the application of a highly efficient halophilic denitrifying bacterium of the genus *Halomonas*. The strain is *Halomonas ramblicola* 5505, a salt-tolerant heterotrophic nitrifying-aerobic denitrifying bacterium, with accession number CGMCC No. 30683. The strain primarily removes ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen from water through assimilation, achieving assimilation rates of 62.68%, 51.08%, and 52.66%, respectively; it can also dissimilate some inorganic nitrogen into nitrogen-containing gases. In simulated nitrogen-containing wastewater, the strain achieved a removal rate of over 90% for ammonia nitrogen and nitrate nitrogen within 12 hours, and increasing the carbon-to-nitrogen ratio completely removed accumulated nitrite nitrogen. This strain effectively solves the problem of inhibition of biological denitrification microorganisms by high-salt nitrogen-containing wastewater. Its nitrogen removal process is green, efficient, and energy-saving, and can be applied to the ecological treatment and restoration of water bodies polluted by high salt and nitrogen.

The application provides a biological organic fertilizer suitable for saline-alkali soil and a preparation method and application thereof, and belongs to the field of microbial fertilizers.The biological organic fertilizer is prepared from halomonas sp ZH-1, including organic acid fermentation liquor, perlite, activated carbon, distilled water, halomonas sp, pancreatin and yeast extract.The biological organic fertilizer can convert insoluble phosphate into soluble phosphate which can be absorbed by plants, can synthesize indole acetic acid by using tryptophan, and has the ability of synthesizing iron carrier.The biological organic fertilizer can improve the chemotaxis distance of halomonas sp, enhance the activity of halomonas sp in soil, improve the colonization ability of the strain in the rhizosphere, improve the stress resistance of wheat under salt stress, promote the growth of wheat, improve the thousand-grain weight of wheat, and finally improve the yield of wheat.

The application discloses Halomonas for producing PHA by using CO2 electrochemical derivatives, a construction method and application of the Halomonas, and the Halomonas expresses Rhs644 genes and Rhs647 genes from Halomonas sp.TD01. The application realizes production of poly-3-hydroxybutyrate by using CO2 electrochemical derivatives as a single carbon source in the Halomonas, without additional adding of antibiotics and inducers, greatly reduces production cost, and improves single batch fermentation income. Meanwhile, efficient utilization of a cheap carbon source is realized, and the process of low-carbon biological manufacturing can be promoted. The poly-3-hydroxybutyrate is produced by using the application, and the time period is short, the production process is simple, the environment is green and pollution-free, and the application is beneficial to industrialized large-scale production.

The application provides a recombinant bacterium for producing 3-hydroxypropionic acid, and a construction method and application thereof. The recombinant bacterium expresses a halomonas sp. derived accA gene, accB gene and malonyl-CoA reductase. The application fully utilizes the 3HB synthesis pathway of the strain itself, cooperates with the introduced 3-HP synthesis path, realizes high-efficiency synthesis of poly(3-hydroxybutyric acid-3-hydroxypropionic acid) or poly(3,4-dihydroxybutyric acid-3-hydroxypropionic acid) without passing through the intermediate product 3-hydroxypropionic acid aldehyde, and avoids the toxic effect of the intermediate product on cells.

The application belongs to the technical field of microorganisms, and discloses a halophilic halomonas and application thereof. Franzmannia qiaohouensis The halomonas is separated from the millet rhizosphere soil of saline-alkali land, has a strain number STM-122, a preservation number CGMCC No. 36349, is identified and classified by 16S rRNA gene sequence identification and phylogenetic analysis, and has the functions of phosphorus solubilization, EPS production, auxin production and iron carrier production. Through seed treatment by bacterial suspension, the germination energy and germination rate of millet seeds under saline-alkali stress can be significantly improved, and the indexes such as seedling root length and plant height are promoted; meanwhile, a complete preparation method of the fermentation bacterial fertilizer of the strain is provided, and the obtained fermentation bacterial fertilizer has a viable bacterial count ≥1×10 8 CFU / g. The application can enrich the salt-tolerant microbial resources, and provide technical support for the planting of millet and other crops in saline-alkali land.

The application discloses a dynamic morphological regulation system of halomonas sp. Halomonas ) and application thereof in intracellular product synthesis. The system comprises a diameter regulation expression module and a length regulation expression module, wherein the diameter regulation expression module comprises a SspB coding gene operably connected with a temperature-sensitive promoter; and the length regulation expression module comprises a MinCD coding gene operably connected with a temperature-sensitive promoter. At a first temperature, SspB and MinCD are not expressed or are expressed at a low level, the cell maintains normal growth and accumulates biomass; after being raised to a second temperature, SspB mediates MreB degradation to increase the cell diameter, and MinCD interferes with Z ring positioning of cell division to increase the cell length, thereby becoming a super-large cell bacterium. The system can also be coupled with a intracellular product synthesis pathway strengthening module to realize comprehensive optimization of "large volume + high content of intracellular product". The application further discloses an engineered bacterium comprising the system, an expression vector and application thereof.

The present invention discloses a L-2,4-diaminobutyrate transaminase mutant and its application in the synthesis of ectoin, which is an amino acid sequence derived from Halomonas sp.7T such as L-2,4-diaminobutyrate transaminase shown in SEQ ID NO:2 as a starting sequence and having an amino acid sequence of corresponding mutation sites (such as E407D or E407K, etc.). The present invention also discloses a coding gene, a vector, a bioengineering bacterium and a method for synthesizing ectoin of the L-2,4-diaminobutyrate transaminase mutant. The advantages of the present invention are good enzyme activity, good thermal stability, and / or high preparation yield.

The invention provides a recombinant bacterium for producing 3-hydracrylic acid as well as a construction method and application of the recombinant bacterium. The recombinant bacterium expresses an accA gene, an accB gene and malonyl-coenzyme A reductase which are derived from halomonas. According to the invention, a 3HB synthesis path of the strain is fully utilized and is cooperated with an introduced 3-HP synthesis path, efficient synthesis of poly (3-hydroxybutyrate-3-hydracrylic acid) or poly (3, 4-dihydroxybutyrate-3-hydracrylic acid) can be realized without an intermediate product 3-hydracrylic aldehyde, and the toxic action of the intermediate product on cells is avoided.

The application provides an organic carrier endo- rhizosphere growth promoting microbial agent for degraded grassland ecological restoration and a preparation method and application thereof, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth promoting microbial agent comprises four endo- rhizosphere growth promoting bacteria (PGPE), four rhizosphere growth promoting bacteria (PGPR) and an organic carrier; the PGPE comprises B. terrigena K4, B. subtilis 9-3-1, B. safensis 11-5-4 and Halomonas sp. L31, the PGPR comprises Serratia marcescens 5, Serratia marcescens 23, Kosakonia koreensis 11 and Enterobacter cloacae 24, and the organic carrier is carbon-based organic fertilizer, cattle and sheep manure organic fertilizer or biochar. The application of the organic carrier growth promoting microbial agent in degraded grassland can significantly improve the biomass and coverage of the plant community. The organic carrier growth promoting microbial agent can be used for the ecological restoration of degraded grassland.

The invention discloses a method for treating high-salt and high-ammonia-nitrogen sewage by using heterotrophic nitrification and aerobic denitrification bacteria, and relates to a method for treating high-salt and high-ammonia-nitrogen sewage, the strain of the method is Halomonas, is named as Halomonas sp.MY-11, belongs to Halomonas, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC M 20242923, and has the preservation date of December 27, 2024. The strain is a heterotrophic nitrification-aerobic denitrification bacterium, can efficiently remove inorganic nitrogen pollution in a water body, still has good denitrification performance under the stress of high salinity and high ammonia nitrogen, and has strong tolerance to high salinity and high ammonia nitrogen. The strain can efficiently treat nitrogen-containing wastewater under the conditions that the salinity is 2%-6% and the ammonia nitrogen concentration is 100 mg / L-400 mg / L, and the removal efficiency can reach 99% or above. The method has great potential in the application of inorganic nitrogen pollution treatment of various water bodies such as culture and chemical sewage in China.

The invention relates to the technical field of biological materials, in particular to engineering modified halomonas, a construction method thereof and a method for producing a tissue filling material by using the engineering modified halomonas. The engineering modified halomonas is obtained by overexpressing a phaAB gene (SEQ ID NO: 2) through a promoter porin 59 (SEQ ID NO: 1) in the Halomonas blue phaseensis CYL37, and the engineering modified halomonas is obtained by overexpressing the phaAB gene (SEQ ID NO: 2) through the promoter porin 59 (SEQ ID NO: 1). The material for tissue filling prepared by the embodiment of the invention has better mechanical properties, and can realize the effects of long-acting filling and skin improvement.

The present invention relates to the technical field of biological fermentation and particularly to a fermentation method for Halomonas sp. and use thereof in the preparation of PHA. Using Halomonas sp. as a fermentation strain, glucose and a derivative thereof as carbon sources, and an acetate (such as sodium acetate) as a pH neutralizer, the method provided by the present invention not only reduces the production cost, but also improves the overall rate of conversion of glucose to PHA or other products during production, achieving the effect of improving dry cell weight and product quality.

The application discloses a heterotrophic nitrification and aerobic denitrification bacteria for treating high-salt and high-ammonia-nitrogen wastewater, and relates to a high-salt and high-ammonia-nitrogen wastewater treatment method. Halomonas sp The bacteria strain is Halomonas sp. Halomonas , named MY-11, belongs to the genus Halomonas, is preserved in the China Center for Type Culture Collection, has a preservation number of CCTCC M 20242923 and a preservation date of December 27, 2024. The bacteria strain is a heterotrophic nitrification and aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water bodies, has good denitrification performance under high-salt and high-ammonia-nitrogen stress, and has high tolerance to high-salt and high-ammonia-nitrogen. The bacteria strain can efficiently treat nitrogen-containing wastewater under the conditions of 2%-6% salinity and 100 mg / L-400 mg / L ammonia-nitrogen concentration, and the removal efficiency can be higher than 99%. The bacteria strain has great potential in the application of inorganic nitrogen pollution treatment of various water bodies such as breeding and chemical industry wastewater.

The application discloses a strain of Lactobacillus plantarum NX3-5 and application thereof, and belongs to the technical field of beneficial bacteria. Halomonas sp. The application discloses a strain of Lactobacillus plantarum NX3-5 and application thereof, and belongs to the technical field of beneficial bacteria. Halomonas sp. The application discloses a strain of Lactobacillus plantarum NX3-5 and application thereof, and belongs to the technical field of beneficial bacteria. The application provides the strain of Lactobacillus plantarum NX3-5, which is preserved in the China Center for Type Culture Collection on December 18, 2025, and has a preservation number of CCTCC M 20252959; the provided strain has high salt and alkali tolerance; has the ability of reducing salt and alkali, and the alkali reduction rate is 18.22 %; further has the abilities of nitrogen fixation, potassium solution, organic phosphorus dissolution, protease production and IAA production, and can significantly promote the growth of the aboveground and underground parts of cucumber plants under normal conditions and salt and alkali stress conditions.

The invention discloses a halophilic hydrocarbon degrading bacterium Halomonas sp. DH1 as well as a mixed preparation and application thereof, and relates to the technical field of microorganisms. The invention specifically discloses a strain of Halomonas sp. DH1, which is preserved in the China Center for Type Culture Collection on June 3, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20241125. The strain can degrade, disperse, emulsify and solubilize crude oil and degrade various petroleum hydrocarbons under the condition of hypersalinity, can generate a surfactant, can effectively improve the fluidity of the crude oil in a stratum, and improves the recovery rate of the crude oil.