The invention discloses halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof , and belongs to the technical field of microorganism application. The invention provides two strains of halomonas sp. MCCC 1A17488 and MCCC 1A13718 with aerobic denitrification and heterotrophic sulfur oxidation functions, and the preservation numbers of the halomonas sp. MCCC 1A17488 and the MCCC 1A13718 are as follows: GDMCC No:60985 and GDMCC No:60984. The halomonas sp. MCCC 1A17488 and MCCC 1A13718 can be applied to nitrogen and sulfur removal, can be suitable for biological nitrogen removal treatment of various nitrogen-containing wastewater, has efficient denitrification and heterotrophic sulfur oxidation capacity under aerobic and anoxic conditions, can rapidly and efficiently remove nitrate, nitrite, ammonia nitrogen, sulfide and the like, and has good application prospects in the biological nitrogen and sulfur removal of sewage, sludge, sedimentsor culture water bodies.