Application of a halomonas sp. strain with high-efficiency salt-tolerant denitrification

By screening and optimizing the growth conditions of Halomonas ramblicola 5505 strain, the problem of low nitrogen removal efficiency of heterotrophic nitrification-aerobic denitrification microorganisms under high salinity conditions was solved, achieving efficient removal of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water bodies, which is suitable for the treatment of high salinity nitrogen-containing wastewater.

CN118929936BActive Publication Date: 2026-03-17LINGNAN NORMAL UNIV
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Patent Information

Application Number
CN202411132007.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-08-19
Publication Date
2026-03-17
Estimated Expiration
2044-08-19

AI Technical Summary

Technical Problem

Existing heterotrophic nitrifying-aerobic denitrifying microorganisms have difficulty in stably performing their denitrification function in high-salt environments, resulting in low treatment efficiency of high-salt nitrogen-containing wastewater. In particular, when the salt concentration exceeds 2%, the activity of biological enzymes is inhibited, and the growth of microorganisms is hindered.

Method used

A strain of Halomonas ramblicola 5505 was screened and applied as a salt-tolerant heterotrophic nitrifying-aerobic denitrifying bacterium, adapted to growth in a salt concentration range of 0%-25%, with an optimal salt concentration of 3%-8%. Carbon source, nitrogen source, carbon-nitrogen ratio, rotation speed, temperature and pH conditions were optimized to achieve efficient nitrogen removal.

Benefits of technology

It achieves highly efficient removal of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water under high salinity conditions, with removal rates of 100%, 100% and 74.4% respectively. It can also convert some inorganic nitrogen into nitrogen-containing gas, with a total nitrogen removal rate of up to 87.89%, thus solving the problem of inhibition of biological denitrification function under high salinity conditions.

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Abstract

This invention relates to the application of a highly efficient halophilic denitrifying bacterium of the genus *Halomonas*. The strain is *Halomonas ramblicola* 5505, a salt-tolerant heterotrophic nitrifying-aerobic denitrifying bacterium, with accession number CGMCC No. 30683. The strain primarily removes ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen from water through assimilation, achieving assimilation rates of 62.68%, 51.08%, and 52.66%, respectively; it can also dissimilate some inorganic nitrogen into nitrogen-containing gases. In simulated nitrogen-containing wastewater, the strain achieved a removal rate of over 90% for ammonia nitrogen and nitrate nitrogen within 12 hours, and increasing the carbon-to-nitrogen ratio completely removed accumulated nitrite nitrogen. This strain effectively solves the problem of inhibition of biological denitrification microorganisms by high-salt nitrogen-containing wastewater. Its nitrogen removal process is green, efficient, and energy-saving, and can be applied to the ecological treatment and restoration of water bodies polluted by high salt and nitrogen.
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