Kit for detecting canine C-reactive protein based on homogeneous chemiluminescence immune competition method

A homogeneous chemiluminescence and reactive protein technology is applied in the field of kits for detecting canine C-reactive protein based on homogeneous chemiluminescence immunocompetitive method, which can solve problems such as inconvenience and achieve accurate results, short time consumption, and shortened turnaround time. Effect

Active Publication Date: 2020-02-25
南京浦光生物科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] This shows that the above-mentioned existing canine C-reactive protein (cCRP) obviously still has inconvenience and defects in the detection method and use, and needs to be further improved urgently.
In order to solve the problems existing in the detection method of canine CRP, relevant manufacturers have tried their best to find a solution, but no suitable design has been developed for a long time, and there is no suitable method to solve the above problems , this is obviously a problem that relevant industry players are eager to solve

Method used

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  • Kit for detecting canine C-reactive protein based on homogeneous chemiluminescence immune competition method
  • Kit for detecting canine C-reactive protein based on homogeneous chemiluminescence immune competition method
  • Kit for detecting canine C-reactive protein based on homogeneous chemiluminescence immune competition method

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Experimental program
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Effect test

Embodiment 1

[0040] Configure detection reagents: mix DNA1-cCRP antigen conjugate (cCRP Ag), DNA2-cCRP antibody conjugate (cCRPAb), AE-modified DNA3, and GO-AOD to make their final concentrations respectively 1nM, 5nM, and 0.05 μM and 20 μg / ml. Mix 20 μL of calibration solution with different concentrations (0, 5, 15, 30, 60, 100, 200 mg / L) with 200 μL of detection solution, place in HSCL-10000 chemiluminescence instrument, and incubate at 37°C for 5 minutes . After incubation, 200 μL of chemiluminescence substrate was added by HSCL-10000 chemiluminescence instrument, and the chemiluminescence signal of the solution was detected by photomultiplier tube (PMT) immediately, and the detection time was 3 s. According to the recorded chemiluminescence value (RLU), the calibration curve of cCRP and the concentration of cCRP in the sample to be tested are obtained. The specific results are shown in Table 1.

Embodiment 2

[0042] Configure detection reagents: mix DNA1-cCRP antigen conjugate (cCRP Ag), DNA2-cCRP antibody conjugate (cCRPAb), AE-modified DNA3, GO-AOD to make their final concentrations respectively 5nM, 10nM, 0.1 μM and 20 μg / ml. Mix 20 μL of calibration solution with different concentrations (0, 5, 15, 30, 60, 100, 200 mg / L) with 200 μL of detection solution, place in HSCL-10000 chemiluminescence instrument, and incubate at 37°C for 5 minutes . After incubation, 200 μL of chemiluminescence substrate was added by HSCL-10000 chemiluminescence instrument, and the chemiluminescence signal of the solution was detected by photomultiplier tube (PMT) immediately, and the detection time was 3 s. According to the recorded chemiluminescence value (RLU), the calibration curve of cCRP and the concentration of cCRP in the sample to be tested are obtained. The specific results are shown in Table 1.

Embodiment 3

[0044] Configure detection reagents: mix DNA1-cCRP antigen conjugate (cCRP Ag), DNA2-cCRP antibody conjugate (cCRPAb), AE-modified DNA3, and GO-AOD to make their final concentrations respectively 1nM, 10nM, 0.1 μM and 20 μg / ml. Mix 20 μL of calibration solution with different concentrations (0, 5, 15, 30, 60, 100, 200 mg / L) with 200 μL of detection solution, place in HSCL-10000 chemiluminescence instrument, and incubate at 37°C for 5 minutes . After incubation, 200 μL of chemiluminescence substrate was added by HSCL-10000 chemiluminescence instrument, and the chemiluminescence signal of the solution was detected by photomultiplier tube (PMT) immediately, and the detection time was 3 s. According to the recorded chemiluminescence value (RLU), the calibration curve of cCRP and the concentration of cCRP in the sample to be tested are obtained. The specific results are shown in Table 1.

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Abstract

The invention discloses a kit for detecting canine C-reactive protein based on a homogeneous chemiluminiscence immune competition method. The kit comprises a DNA1-cCRP antigen conjugate, a DNA2-cCRP antibody conjugate, acridinium ester (AE) labeled DNA3 and a graphene oxide (GO) binding antioxidant (AOD). The kit provided by the invention is used for detection, and the kit has the advantages of simple operation, accurate result, short time consumption, high precision and the realization of quantitative detection, a sample does not need to be diluted, and a HOOK effect does not exist.

Description

technical field [0001] The invention relates to the technical field of chemiluminescence immunoassay, in particular to a kit for detecting canine C-reactive protein based on a homogeneous chemiluminescence immunocompetition method. Background technique [0002] In recent years, because human CRP plays a very important role in clinical disease diagnosis, disease course detection and prognosis, the research on CRP has become a hot spot in current human clinical medical research. Although the application of CRP in human medicine has been very extensive and in-depth, and its detection technology is also very mature, CRP has not been widely used in the routine detection of veterinary clinics, and the research on the change of CRP concentration in diseases is only in the laboratory research stage. In foreign countries, as early as the 1970s, clinical research on CRP in dogs has been carried out, but there are few documents in China. [0003] According to the literature, like hum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/76
CPCG01N21/76G01N33/6893G01N2333/4737
Inventor 曹丹
Owner 南京浦光生物科技有限公司
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