The invention provides a method for preparing a polyimide nano-film. The method comprises the steps: firstly, synthesizing polyimide resin from aromatic tetracid dihydride, aromatic diamine and aromatic diisocyanate; and then, mixing the polyimide resin, a polar organic solvent, a diluent, a film forming agent and a molecular weight regulator so as to obtain a polyimide solution system, then, carrying out nano-scale uniform spreading on a clean substrate by dint of the action of a mechanical external force of 0.5N to 30N, and then, carrying out an imidization method, thereby preparing the polyimide nano-film. According to the film, the high-temperature resistance is 10 seconds at the high temperature of 400 DEG C to 750 DEG C, and the electrical insulation property is that the dielectric constant is 1.5 to 4.5 at the frequency of 103Hz. Compared with the prior art, the polyimide nano-film prepared by the method has the advantages of excellent surface physical properties and stable chemical properties, high-temperature resistance, electrical insulation properties and chemical corrosion resistance.