49results about How to "Reduce the chance of cross-contamination" patented technology

The invention relates to a centrifugal microfluidic serum separation chip and a preparation method thereof. The centrifugal microfluidic serum separation chip is a wafer-shaped microfluidic chip with a micro-structure and a micro-channel, and consists of a chip engraved with a micro-structure and a micro-channel and a blank chip. The centrifugal microfluidic serum separation chip is fixed on a rotation platform of a centrifugal machine, the separation operation of serum and erythrocyte in a micro-upgraded blood sample can be realized by the synergetic process of the micro-structure and the micro-channel on the chip, and the serum for analysis and detection can be acquired. The centrifugal microfluidic serum separation chip is applicable in related application fields, such as biomedicine, clinical diagnosis, health quarantine and the like.

The invention discloses a full-automatic continuous kitchen garbage vacuum compressing and packing device. The full-automatic continuous kitchen garbage vacuum compressing and packing device comprises a packaging mechanism, a liquid draining pipe, an air exhaust mechanism, a garbage collecting barrel, a form machine, a winding drum and a base. The garbage collecting barrel is fixed to an installing seat through an installing support; the installing seat is fixed to the base; the winding drum is installed on the installing seat and located on one side of the garbage collecting barrel; and one end of the form machine embraces the outer wall of the garbage collecting barrel, and the other end of the form machine is connected with the winding drum for guiding a plastic thin film on the winding drum onto the outer wall of the garbage collecting barrel to form a pipy garbage collecting bag. Garbage is vacuum-packed and collected from a generation source of the kitchen garbage, the process of collecting the kitchen garbage is simplified, mixing of the kitchen garbage and other domestic garbage is avoided, cross pollution is prevented, protecting of the environment is strengthened, meanwhile protecting of garbage treatment personnel is improved, and the daily workloads of family members are reduced.

The invention provides a probe, a kit and a method for detecting R47H mutation of a TREM2 gene. The probe can detect whether base G on rs75932628 locus of the TREM2 gene is mutated to A. The probe can realize single tube enclosing and rapidly and simply detect R47H mutation of the TREM2 gene in a sample, and greatly reduces the chance of cross contamination; the method has simple operation and fast detection only requiring 1.5 h; and the detection results are intuitive, and three genotypes of TREM2 in a sample can be determined qualitatively according to different fluorescence signals, without complex analysis process.

The invention relates to a multi-axis robot control system and a multi-axis robot control method. The control system comprises a main control board, wherein the main control board comprises a manipulator interface, a memorizer, a controller and a servo driver interface, wherein the manipulator interface is used for connecting a multi-axis manual and synchronous manipulator so as to transmit the control information sent by the multi-axis manual and synchronous manipulator; the memorizer is used for storing the control information sent by the multi-axis manual and synchronous manipulator and optimizing trajectory data; the controller is used for calculating the stored control information to obtain coordinate information of a transition point and a target point and optimize a moving trajectory, and used for storing well optimized trajectory data into the memorizer and sending to the servo driver to control the multi-axis robot to move on the optimized trajectory; and the servo driver interface is used for connecting a servo driver. The invention further discloses the multi-axis robot control method. The multi-axis robot control system provided by the invention has the advantages of absence of hysteresis, high accuracy and simplicity in calculation, and can realize true meaning of no programming robot use.

The invention provides an RT-PCR primer used by a lily symptomless virus test method and a test kit thereof; wherein an upstream primer LSV-P1 is: 5'-GAY GAR YTY AAR ATG AAR GT-3'; a downstream primer LSV-P2 is: 5'-ARY TGY TTR TGY GCR TTR TG-3'; wherein R is A or G; and Y is C or T. Using the test kit of the invention to test can greatly reduce the possibility of cross contamination, improve the test efficiency to a large extent, ensure high test sensitivity and simple and convenient operation, and prevent cross contamination.

The invention relates to the field of a biological medicine, and particularly relates to a real-time fluorescent PCR (polymerase chain reaction) detection kit for non-deletion type alpha thalassemia. The kit comprises two PCR reagents, namely a PCR reagent I and CPR reagent II, wherein each PCR reagent comprises a primer, a probe, PCR Buffer, dNTPs, MgCl2, DNA (deoxyribonucleic acid) polymerase, UNG (unguentum) enzyme and glycine betaine. By adopting the kit, QS (quality safety) and CS mutant types of the non-deletion type alpha-thalassemia can be simultaneously detected by a single test. The real-time fluorescent PCR detection kit has the characteristics of short detection time, high sensitivity and strong specificity, and is simple to operate; the result can be judged and read after fluorescent PCR is finished; the problem of carrying out complicated operations of hybridization, sequencing and solubility curve analysis on a PCR product in the prior art is overcome.

The invention provides a PCR detecting primer and a PCR detecting kit of cucumber mosaic virus, wherein, an upstream primer CMV-P1:5'-CACCCAACCTTTGTGGGTAG-3' and a downstream primer CMV-P2:5'-CAACACTGCCAACTCAGCTC-3' are adopted. The PCR detecting kit of the invention can greatly lower cross-contamination probability in detection, remarkably raises detection efficiency, has ultrahigh detection sensitivity as well as simple and convenient operation, and prevents cross-contamination.

The invention discloses a liquid level detection device and a liquid level detection method. The liquid level detection device comprises a signal generation circuit, a signal processing circuit and asignal processing circuit, wherein the reference circuit and the sampling circuit respectively receive signals with preset frequency and preset waveform and output corresponding first signals and second signals, and the sampling circuit is connected with the liquid level detection needle; the comparison circuit is connected with the reference circuit and the sampling circuit so as to receive the first signal and the second signal and generate a corresponding comparison result signal; the processing circuit is connected with the comparison circuit to receive the comparison result signal, and when the processing circuit determines that the difference value between the output voltage corresponding to the comparison result signal and the threshold voltage is larger than or equal to the set voltage, a liquid level confirmation signal is generated to determine that the liquid level detection needle makes contact with the liquid level, wherein the threshold voltage is determined according toa comparison result signal received by the processing circuit when the liquid level detection needle is not in contact with the liquid level. In this way, the liquid level detection precision can be improved, and the response time can be shortened.

The invention belongs to the technical field of plant virus detection, and in particular relates to PCR primers for detecting a cactus X virus and applications of the PCR primers. The primers comprise an upstream primer CVX-CPF: 5'-CAGTCCGCCGGACCCCG-3', and a downstream primer CVX-CPR: 5'-AGACTCTGAACCTTGGAGCCCC-3'. The PCR primers provided by the invention have certain universality for detecting CVX, can be used for detecting the CVX virus on dragon fruits, and also can be used for detecting the CVX virus in cactus. According to a detection method for detecting the CVX virus on dragon fruits, the inverse transcription and PCR amplification are carried out in one step, thus the probability of cross contamination can be greatly reduced, and meanwhile, the detection efficiency is greatly improved. The practical applications show that the operation is simple and convenient, the detection sensitivity is relatively high, and about 1 pg can be detected.

The detection device comprises the body and the detachable box including at least one detector tube column. The analytic system comprises the host instrument and the handhold electrophoresis detection device. The host instrument controls the process for testing the sample. The handhold electrophoresis detection device can connect to the host instrument in detachable manner electrically. The detector tube column possesses the first end and the second end. The first voltage can be applied to the first end and the second voltage can be applied to the second end. With the sample being attached to the detector tube column, based on the difference between the first voltage and the second voltage, the sample is moved from the second end to the first end in the detector tube column.

The invention relates to a food-grade raw material circulation and sterilization channel system. The food-grade raw material circulation and sterilization channel system comprises a channel, a conveying device, a material inlet, a material outlet, a sterilization device and an induction device, wherein the conveying device is arranged at the center of the channel; the material inlet and the material outlet are arranged at two ends of the channel; the sterilization device and the induction device are arranged on an inner wall of the channel; the sterilization device comprises sterilization lamps and an exhausting nozzle; the plurality of sterilization lamps are arranged; the sterilization lamps are fixedly arranged at two sides and the top of the inner wall of the channel; the exhausting nozzle is arranged at the top of a channel at a material outlet end; the direction of the exhausting nozzle points to the material inlet; the exhausting nozzle is connected with a draught fan; the draught fan is connected with a sterile air source. The food-grade raw material circulation and sterilization channel system provided by the invention has the beneficial effects that the system can be usedfor sufficiently sterilizing materials and creating a sterile transportation environment in a high-standard manner; sanitation and safety in a food raw material transportation process are sufficiently ensured; the system can be used for connecting material transportation between a common region and a super-clean region; air is circulated in the channel so that raw materials with different types are transported and the cross contamination probability also can be reduced.

The invention discloses a CYP2C19 gene polymorphism detection kit and an application of thereof. The kit comprises forward and reverse primers for detecting CYP2C19 * 2 (G681A), a wild type detectionprobe and a mutant type detection probe, wherein the forward and reverse primers, a wild-type detection probe and a mutant-type detection probe are used for detecting CYP2C19*3 (G681A), and a wild-type quenching probe and a mutant-type quenching probe shared by two sites, wherein the wild-type detection probe and the mutant-type detection probe are respectively used for marking different fluorescent groups. The specificity of probe binding is improved by utilizing a locked nucleic acid technology, the polymorphism of the CYP2C19 gene is detected by combining a multiple fluorescent PCR technology, the operation is convenient and rapid, the detection result is easy to interpret, the specificity is strong, the accuracy and the sensitivity are high, and an effective basis is provided for clinical medication of clopidogrel.

The invention discloses an automatic separate collection device for micro samples. The device comprises a worktable, wherein the worktable is provided with a suspended mechanical arm; a horizontal suspension is arranged at the top end of the suspended mechanical arm; the horizontal suspension is located above a suspension liquid sucking and drying part; a dot matrix bottle rack is arranged at thebottom of the worktable; a plurality of chromatographic sample injection bottles can be placed in the inner cavity of the dot matrix bottle rack; a liquid shunt is arranged above the horizontal suspension; and a conveying pipe, a waste liquid pipe and a connecting pipe are respectively arranged on the liquid shunt. According to the device, high automation can be realized; during the operation, collection is carried out according to a pre-designed program; dozens of different sample components can be collected at one time; the small and micro sample components can be accurately collected; the volumes of collected liquid are far smaller than a common liquid drop; the collected samples are stored in the enclosed chromatographic sample injection bottles; and the enclosed environment not only can protect the samples, but also can decrease the cross contamination of the samples.

The invention discloses a probe, a kit and a method thereof for detecting MAPT gene mutation, and relates to the field of bio-medicine. The probe for detecting the MAPT gene mutation includes, but not limited in, at least one of a TaqMan probe, a TaqMan-MGB probe, a molecular beacon, a modified molecular beacon, a double-stranded fluorescent displacing probe, a LightCycler probe, a double-loop probe and the like. The kit for detecting the MAPT gene mutation consists of an amplification primer, a fluorescent probe, an amplification reagent and a reference reagent. The method for detecting the MAPT gene mutation comprises the following steps: 1) selecting a sample; 2) extracting genome DNA; 3) conducting real-time fluorescent PCR amplification and detection; and 4) conducting result analysis. Experimental results can be observed in real time. Meanwhile, the probe, the kit and the method are more sensitive and rapid in detection, more convenient to operate and easy for automation, and the probability of cross pollution can be greatly reduced and the accuracy of detection can be improved. Cost is saved, and meanwhile flux is improved.

The invention discloses primers, a kit and a detection method for detecting a bean yellow mosaic virus. The sequences of the primers disclosed by the invention are BYMV Primer-1:5'-ccaacattccgccaaataat-3', and BYMV Primer-2:5'-aatacgaacaccaagcatgg-3'; and the kit and the detection method contain a primer BYMV Primer-1 and a BYMV Primer-2. The primers and the detection method for detecting the bean yellow mosaic virus disclosed by the invention are high in accuracy, strong in specificity, and high in sensitivity.

The invention provides a high-temperature baking sterilizer and relates to the technical field of sterilizers. A feeding/discharging port is formed in the front side of a drying box casing and a sealing door is in rotary connection with one side of the feeding/discharging port; a rotating shaft is in rotary connection in the drying box casing, an electrical cabinet is arranged at the top of a pulling shaft, and a plurality of switch knobs are arranged on the outer wall of the electrical cabinet; a plurality of partition plates are arranged on the circumferential wall of the rotating shaft and a plurality of placing plates are arranged between the adjacent partition plates; a total threading hole is formed in the rotating shaft; a plurality of branched threading holes are formed in the placing plate and a total wire penetrates into the total threading hole; branched wires are arranged in the branched threading holes; the total wire is connected with the branched wires; the plurality of switch knobs are all connected with a main circuit board in the electrical cabinet; and sealing layers are arranged at the periphery of the partition plates, and the outer walls of the sealing layers are contacted with the inner wall of the drying box casing. The high-temperature baking sterilizer can sterilize different strains simultaneously; furthermore, the sterilized strains can be taken out conveniently, the cross contamination probability of different strains is reduced and the application range is wider.