Cell culture bottle for co-culturing attached cells and suspension cells

Abstract

The invention discloses a cell culture bottle for co-culturing attached cells and suspension cells. The cell culture bottle comprises a bottle body, wherein the bottle body is provided with a bottle opening and a bottle cap; the bottle body is internally provided with a nano semipermeable membrane; the nano semipermeable membrane is used for separating a space in the bottle body into two parts which are used as a culture container of the attached cells and a culture container of the suspension cells respectively; the bottle cap is an air permeable bottle cap. According to the cell culture bottle disclosed by the invention, the two types of cells do not influence each other in an operation process; however, cell factors secreted between the two types of cells can interact through the nano semipermeable membrane, and can really reflect mutual effects and influences between the two types of cells in a certain sense.

Description

technical field [0001] The invention relates to the field of biological experiment consumables, in particular to a cell culture bottle for co-culture of adherent cells and suspension cells. Background technique [0002] Cell culture flasks, as the name suggests, are used to culture cells. However, all cultures currently on the market can be classified into glass and plastic according to the material, and the bottom shape is square, rectangular, triangular, etc., and there are 5 to 500ml of bottles to choose from according to the volume of the bottle. In addition, after the surface is specially modified, the cells can be better adhered to the culture, so that according to the area of ​​the cells adhered to the wall, it can be divided into 25 ~ 175cm 2 Various. However, there is no cell flask that can co-culture two different culture types (adherent cells and suspension cells) at the same time. [0003] A semi-permeable membrane is a membrane that only allows certain molecu...

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