Primers, probes and detection kit for detection of DPYD*2A genetic polymorphism

Abstract

Relating to in-vitro nucleic acid detection, the invention provides primers, probes and a detection kit for detection of DPYD*2A genetic polymorphism. Different fluorescence group labeled probes can be used for detecting the polymorphism of gene DPYD*2A (rs3918290) in a sample. Single tube closing can achieve simultaneous detection of multiple SNP sites, the defect of low detection flux in the traditional fluorescence method can be overcome, the probability of cross contamination can be reduced, the operation is simple and the detection speed is fast. Qualitative judgment of various genotypes in a to-be-detected sample can be achieved according to amplification curves produced by different fluorescence signals, and no tedious analysis process is needed. The kit provided by the invention has the advantages of high sensitivity, accurate typing, and simple and convenient operation, etc., can provide effective medication guidance on tumor patients, improve the curative effect of drugs, and reduce adverse reaction of drugs, thus providing basis for realizing individualized treatment of tumor patients.

Description

technical field [0001] The invention relates to in vitro nucleic acid detection, in particular to primers, probes and detection kits for detecting DPYD*2A gene polymorphisms using fluorescence quantitative PCR (FQ-PCR) technology. Background technique [0002] Dihydropyrimidine dehydrogenase (DPD) is one of the key enzymes in the metabolic pathway of fluorouracil (5-FU), which can catalyze the metabolism of 5-FU into non-anticancer active before forming cytotoxic nucleic acids. The metabolite is the rate-limiting enzyme for the metabolic inactivation of 5-FU. DPD is encoded by the dihydropyrimidine dehydrogenase gene (DPYD). The DPYD gene is located on chromosome lp22, with a total length of about 950kb, including 23 exons. The mutation of bases in the DPYD sequence may cause changes in the structure and activity of DPD. The lack of DPD activity is closely related to the toxicity of fluorinated pyrimidine chemotherapy drugs, and the mutation of DPYD plays an important role ...

AI Technical Summary

Problems solved by technology

However, due to the non-sequence specificity of the dye, only singleplex PCR can be performed, which not only reduces the throughput, but also must rely on melting curve analysis to distinguish target fragments from dimers

However, the current probe method is also single-plex PCR, and the throughput is low.

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