Application of iridoid in preparation of anti-osteoporosis medicines

An iridoid and osteoporosis technology, which is applied in the field of medicine, can solve the problems of not knowing the active substances of Ligustrum lucidum, not deeply researching the active ingredients of Ligustrum lucidum, etc., so as to promote the differentiation of osteocytes and promote the proliferation of cells. , the effect of promoting alkaline phosphatase activity

Inactive Publication Date: 2012-03-28
魏园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on the anti-osteoporosis activity of Ligustrum lucidum mostly focuses on the crude extract of Ligustrum lucidum, as disclosed in CN200710073273.9 in the new medical application of Ligustrum lucidum and its pharmaceutical preparations, it is recorded that Ligustrum lucidum is used in the preparation of prophylaxis or The application of drugs for the treatment of osteoporosis, but there is no in-depth study of the specific active ingredients in Ligustrum lucidum, and it is not known which active substance in Ligustrum lucidum can prevent or treat osteoporosis

Method used

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  • Application of iridoid in preparation of anti-osteoporosis medicines
  • Application of iridoid in preparation of anti-osteoporosis medicines
  • Application of iridoid in preparation of anti-osteoporosis medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Tracking separation of anti-osteoporosis active ingredients

[0042] UMR-106 cells belong to the rat osteosarcoma cell line and have many characteristics of osteoblasts. Many studies have proved that UMR-106 cells are a good cell model, which can be used to study the mechanism of action of various hormones and growth factors regulating osteoporosis at the cellular level. Compounds that can promote the activity of alkaline phosphatase (ALP) and cell proliferation in UMR-106 cells indicate that they can promote the function of osteoblasts. Estradiol was found to promote ALP activity in UMR-106 cells and was therefore used here as a positive control.

[0043] 1.1 In vitro culture of UMR-106 cells

[0044] Osteoblast UMR-106 was cultured in a 4.5 × 9 cm cell culture flask at 37 °C, constant 5% CO 2 in the cell culture incubator. The cell culture flasks were taken out of the cell culture incubator every 48 h, placed under an inverted microscope to observe th...

Embodiment 2

[0106] Example 2 Effect of Promoting UMR-106 Cell Proliferation

[0107] Using Am-blue cell proliferation and activity detection reagent to detect the proliferation of UMR-106 osteoblasts, and compared with the blank control (only the same amount of solvent DMSO was added), the effects of various extracts and compounds on the proliferation of UMR-106 cells were detected. effect.

[0108] 2.1 Principle of Am-blue cell proliferation assay

[0109] Alamar Blue cell proliferation detection method is a newly developed cell proliferation detection method. Its principle is that during the process of cell proliferation, the environment in the cell body changes from an oxidative environment to a reducing environment. NADPH / NADP, FADH / FAD in the respiratory chain , the ratio of FMNH / FMN and NADH / NAD increased. After Alamar Blue is endocytosed by cells, it is reduced by the above metabolic intermediates in the cytoplasm, and finally released to the outside of the cell. The acc...

Embodiment 3

[0122] Example 3 Ability to scavenge DPPH free radicals

[0123] Absorbance value A detected after reacting each compound with different concentrations with DPPH free radical i , the absorbance value of the respective control tube A j , the absorbance value of the blank tube A b And standard tube absorbance value A 0 Substituting formula (1) to calculate the scavenging rate of each concentration of each compound to DPPH free radicals, take the scavenging rate as the ordinate, and the concentration of each compound as the abscissa plot to obtain the regression equation, and calculate the half free radical scavenging rate of each compound to DPPH Concentration IC 50 When the concentration reaches 500 mg / L, the scavenging rate of DPPH free radicals does not reach 50%, and the result is expressed by the maximum DPPH free radical scavenging rate when the concentration is 500 mg / L. The results are shown in Table 5 . compound 1 and 3 Has a strong ability to scavenge DPPH fre...

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Abstract

The present invention relates to an application of iridoid in the preparation of anti-osteoporosis medicines, which belongs to the medicines technical field. The invention provides an application of iridoid which is shown in a general formula I in the preparation of medicines used for treating and/or preventing osteoporosis. Experiments found that ALP activity in UMR106 cells is taken as guidance for tracing and separating an extract of iridoid possessing general formula I, the extract and each separated component are researched from the aspects of osteoblast propagation and antioxidation activity, the iridoid possessing the general formula I has the effects of substantially promoting alkaline phosphatase activity and osteoblast differentiation, so that the iridoid has anti-osteoporosis effect and can be used for preparing the medicines used for treating or preventing osteoporosis. The component of specnuezhenide and a compound G13 possesses the effects of substantially promoting the alkaline phosphatase activity and osteoblast differentiation, and the specnuezhenide has effects for obviously promoting UMR-106 cell proliferation, a better effect is obtained when the specnuezhenide is used for anti-osteoporosis effect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of iridoid compounds in the preparation of anti-osteoporosis drugs. Background technique [0002] Osteoporosis is a disease characterized by damage to the microstructure of bone tissue, a decrease in the proportion of bone mineral composition and bone matrix, bone thinning, a decrease in the number of bone trabeculae, increased bone fragility, and increased risk of fracture. Diseases of systemic bone metabolism disorders. Osteoporosis has become an important issue affecting the health of our people and social medical security, and has become the second leading cause of death in the elderly, second only to cardiovascular and cerebrovascular diseases. According to the survey, it is estimated that in 2006, about 69.44 million people (15.34 million males and 54.1 million females) suffered from osteoporosis, and about 213.9 million people had low bone mas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P19/10G01N30/90
Inventor 王飞陈前锋杨莉娟张国林魏园
Owner 魏园
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