The invention discloses a kangaroo bone extract and an extraction method thereof, wherein the extraction method comprises: taking kangaroo bone, cleaning, grinding, carrying out acid digestion to demineralize, adopting a protein extraction solution to further carry out multiple digestions, adopting organic solvent leaching to remove impurities, and drying to obtain kangaroo bone extract protein particles. With the extraction method, per g of the protein particles contains 9.5-10.7 mg of protein, 4.8-6.13 mg of osteocalcin, and 4.35*10<-3> ng of bone morphogenetic protein; and mixtures of more than 500 proteins can be extracted from the kangaroo bone, wherein the proteins comprise osteocalcin, osteonectin, bone morphogenetic protein, alkaline phosphatase, insulin-like growth factor and the like, wherein more than 30 proteins are related to bone metabolism, provide ideal promotion effects for induction of differentiation of mesenchymal stem cells into bone cells and cartilage cells, and can be used for preventing and treating bone joint diseases such as osteoporosis and the like. In addition, the kangaroo bone extract protein particles obtained by using the extraction method contain a lot of proteins and related effective components, an extraction effect is good, the kangaroo bone extract protein particles can be used as base raw materials of health products and drugs for prevention and treatment of bone joint diseases, and qualities and effects of kangaroo products are increased.