Kangaroo bone extract and extraction method thereof

A technology of kangaroo osteoin and extraction method, which is applied in bone diseases, pharmaceutical formulations, food science and other directions to achieve good extraction effect and improve market quality and effect.

Active Publication Date: 2013-10-02
南京优而生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, kangaroo bone contains more active ingredients, but it has not been well developed.

Method used

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  • Kangaroo bone extract and extraction method thereof
  • Kangaroo bone extract and extraction method thereof
  • Kangaroo bone extract and extraction method thereof

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Embodiment 1

[0025] A method for extracting kangaroo bone comprising the following steps:

[0026] (1) Select the bones of healthy kangaroos. Because the Australian kangaroo industry is relatively developed, and the population of the eastern gray kangaroo (Macropus giganteus) is relatively large, and the processing industry of kangaroo meat and kangaroo skin is relatively developed, it is selected as the raw material for kangaroo bones. Use a sharp knife to remove the soft tissue connected to the kangaroo bone. After washing with PBS, chop and crush to obtain kangaroo bone fragments. The length and width of the fragments are 2-10 mm, and the thickness is 1-2 mm. .

[0027] (2) Add hydrochloric acid solution to the kangaroo bone fragments, the concentration of hydrochloric acid solution is 1.2mol / L, add 6-8ml per gram of kangaroo bone fragments, cover and digest at 4°C for 12 hours to demineralize, wash with pure water for 3 After several times, pipette the washing solution to obtain demin...

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Abstract

The invention discloses a kangaroo bone extract and an extraction method thereof, wherein the extraction method comprises: taking kangaroo bone, cleaning, grinding, carrying out acid digestion to demineralize, adopting a protein extraction solution to further carry out multiple digestions, adopting organic solvent leaching to remove impurities, and drying to obtain kangaroo bone extract protein particles. With the extraction method, per g of the protein particles contains 9.5-10.7 mg of protein, 4.8-6.13 mg of osteocalcin, and 4.35*10<-3> ng of bone morphogenetic protein; and mixtures of more than 500 proteins can be extracted from the kangaroo bone, wherein the proteins comprise osteocalcin, osteonectin, bone morphogenetic protein, alkaline phosphatase, insulin-like growth factor and the like, wherein more than 30 proteins are related to bone metabolism, provide ideal promotion effects for induction of differentiation of mesenchymal stem cells into bone cells and cartilage cells, and can be used for preventing and treating bone joint diseases such as osteoporosis and the like. In addition, the kangaroo bone extract protein particles obtained by using the extraction method contain a lot of proteins and related effective components, an extraction effect is good, the kangaroo bone extract protein particles can be used as base raw materials of health products and drugs for prevention and treatment of bone joint diseases, and qualities and effects of kangaroo products are increased.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kangaroo bone extract and an extraction method thereof. Background technique [0002] Kangaroo is a unique species in Australia, and the kangaroo industry has also been valued. At present, kangaroo leather and kangaroo meat have been widely developed, while kangaroo bone is rarely used. Although some companies sell kangaroo bone capsules in the market, this health product is only made through simple grinding, which is expensive and has low health effects. . [0003] Animal bones contain osteocalcin, osteonectin, bone morphogenic protein, alkaline phosphatase, insulin-like growth factor, etc., which have good effects on calcium supplementation, prevention and treatment of osteoporosis, and physical fitness enhancement, and can be used for calcium supplementation , to treat osteoporosis. And contain more active ingredients in the kangaroo bone, but not well developed. Contents o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32A23L1/29A61P3/02A61P3/14A61P19/10A23L33/00
Inventor 屈贤琴金星亮杨五宁金肖强
Owner 南京优而生物科技发展有限公司
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