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Kit for measuring content of osteocalcin, and detection method thereof

A kit and osteocalcin technology, applied in measurement devices, biological tests, material inspection products, etc., can solve the significance discount of detection, the instability of osteocalcin, and the lack of sufficient sensitivity to judge the treatment effect of osteoporosis. problems such as reliability and timeliness, to achieve the effect of reliable test results and stable content

Pending Publication Date: 2019-11-01
NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current diagnosis of osteoporosis is mainly through dual-energy X-ray equipment to measure bone mineral density or enzyme-linked immunoassay, but dual-energy X-ray equipment to measure bone mineral density is not sensitive enough for fracture risk prediction and osteoporosis treatment evaluation. and timeliness, there are certain limitations; there are quantitative detection kits based on ELISA on the market, but the significance of detection is greatly reduced due to the instability of osteocalcin in blood

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A test kit for determining the content of osteocalcin, comprising magnetic particle-coupled N-MID capture antibody, enzyme-labeled N-MID detection antibody, N-MID calibrator, enzyme-acted chemiluminescent substrate, and cleaning solution.

[0023] Preferably, the N-MID calibrator is diluted with buffer, and the concentration is 0ng / mL, 0.5ng / mL, 5ng / mL, 10ng / mL, 50ng / mL, 100ng / mL, 500ng / mL, 1000ng / mL In mL, the buffer is Tris or PBS or HEPES buffer containing 0.5-5.0% BSA.

[0024] Preferably, the preparation method of the magnetic particle-coupled N-MID capture antibody is as follows: the magnetic particle is washed with a buffer and then dispersed; EDC and NHS are added, after stirring evenly, the N-MID capture antibody is added, and stirred overnight at 4 °; Magnetically separate the magnetic particles, remove the supernatant, wash the magnetic particles with a buffer solution and disperse; add a blocking solution to the redispersed magnetic particle solution to seal...

Embodiment 2

[0036] The kit prepared in Example 1 is compared with the performance of the chemiluminescent kit (purchased from Roche)

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PUM

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Abstract

The invention discloses a kit for measuring the content of osteocalcin, and a detection method thereof. The kit comprises a magnetic particle coupled N-MID capture antibody, an enzyme labeled N-MID detection antibody, an N-MID calibration product, a chemiluminescence substrate of enzyme action, and cleaning fluid; a whole segment of osteocalcin is generally detected by using euzymelinked immunosorbent assay and radioimmunoassay at home, since the own stability of the osteocalcin is poor, the specimen storage temperature and time will affect the experimental result, N-mid-end osteocalcin (N-MID) is not easily hydrolyzed by protease, so that the content in the serum is stable, the kit disclosed by the invention is used for detecting the N-MID in the serum, and the detection result is reliable.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a kit for measuring the content of osteocalcin and a detection method thereof. Background technique [0002] Osteocalcin, also known as γ-carboxyglutamic acid protein or vitamin K-dependent protein, is the most important specific non-collagen protein in bone matrix, which is synthesized and secreted by osteoblasts in bones and teeth. Binding depends on vitamin K. Osteocalcin molecule contains 49 amino acids with a molecular weight of about 5800D. In bone synthesis, osteoblasts produce osteocalcin, which depends on vitamin K, and vitamin D3 can promote the production of osteocalcin. The use of vitamin K antagonists can reduce the content of this protein in bone, but it does not affect its proline content, nor does it affect the mechanical strength of bone. After the production of osteocalcin, part of it enters the bone matrix, and the other part is released into the peripheral...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N33/535G01N21/76
CPCG01N33/68G01N33/54326G01N33/535G01N21/76
Inventor 周义正唐静崔丛丛
Owner NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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