Bone calcium extraction method by double fermentation and bone gla protein

An extraction method and a secondary fermentation technology, applied in the field of food processing, can solve the problems such as the difficulty of removing the smell of fishy mutton and the poor taste of the final product, and achieve the effect of product aroma and good taste.

Active Publication Date: 2013-01-09
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the bone calcium prepared by the above process, although the content of free calcium increases signifi...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation medium: MRS medium: peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween -80 0.1%, triammonium citrate 0.2%, pH5.5~6.0, 115℃, sterilized for 30min.

Embodiment 2

[0032] Bone crushing and sterilization; take Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of the three kinds of lactic acid bacteria is 1:3:2; aggregate and water The weight to volume ratio is 1:5, the weight ratio of lactic acid bacteria to the aggregate is 0.04%, add 5% sucrose of the total weight, ferment at 50°C for 12h, and sterilize at 120°C for 30min; add hydrochloric acid to adjust the pH value to 3, add pepsin, The weight ratio of the aggregate is 0.08%, the hydrolysis temperature is 40°C, stirring for 2 hours, adding alkali to adjust the pH value to 7, adding papain, accounting for 0.08% of the aggregate weight ratio, stirring at 60°C for 2 hours, heating to kill the enzyme; adding the total weight 5% of glucose, Leuconostoc creamis and citric acid were added, the weight ratio of Leuconostoc creamis to the aggregate was 0.02%, the weight ratio of Leuconostoc creamis and citric ...

Embodiment 3

[0034] Bone crushing and sterilization; take Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of the three kinds of lactic acid bacteria is 1:3:2; aggregate and water The weight to volume ratio is 1:3, the weight ratio of lactic acid bacteria to the aggregate is 0.01%, add 5% sucrose of the total weight, ferment at 35°C for 12h, and sterilize at 120°C for 30min; add hydrochloric acid to adjust the pH value to 1, add pepsin, The weight ratio of the aggregate is 0.04%, the hydrolysis temperature is 35°C, stirring for 1h, adding alkali to adjust the pH value to 6, adding papain, accounting for 0.04% of the aggregate weight ratio, stirring for 1h at 40°C, heating to kill the enzyme; adding the total weight 5% glucose, add Leuconostoc creatis and citric acid, Leuconostoc creatis accounts for 0.06% by weight of the aggregate, Leuconostoc creatis and citric acid weight ratio is 1:20, the fermentati...

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Abstract

The invention discloses a bone calcium extraction method by double fermentation. The bone calcium extraction method by double fermentation includes the steps of fermenting before enzymolysis, enzymolysis and fermenting after enzymolysis. Free calcium content of bone gla protein made by the bone calcium extraction method compared with the prior art is increased greatly. The obtained bone gla protein has fine taste and fragrance, and peculiar odors in extract are removed.

Description

technical field [0001] The invention relates to a bone calcium extraction process and belongs to the field of food processing. Background technique [0002] With the aging of society, the prevalence of osteoporosis ranks first among metabolic bone diseases in western countries. The pain that osteoporosis brings to patients and families is self-evident. According to statistics, there are currently more than 100 million osteoporosis patients in China, and it is estimated that the number will increase to 210 million by 2050. Factors causing calcium deficiency in more and more people: Accelerated pace of life and work, high work pressure and irregular life, not paying attention to the reasonable collocation of dietary structure, those with a daily calcium intake of less than 600 mg can be considered as calcium intake Insufficient calcium in the body can easily lead to lack of calcium in the body; some middle-aged people are stressed at work, lack self-care awareness, and do no...

Claims

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Application Information

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IPC IPC(8): A23L1/312A23L1/304A23L13/20
Inventor 黄永亮吴益群赵健崔卜东郁正刚
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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