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Bone calcium extraction method and bone gla protein

An extraction method and aggregate technology, applied in application, food preparation, food science, etc., can solve the problems of high fat content in products and difficulty in removing bone fat

Active Publication Date: 2014-03-05
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the bone calcium prepared by the above process, the degreasing method is mainly implemented by cooking, which is difficult to remove the high content of fat in the bone, resulting in a higher fat content in the final product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take animal bones and crush them to 40mm particles, add ethanol, extract at 80°C for 75min, discard the supernatant; soak and rinse in hot water at 90°C for 30min, centrifuge the aggregate at 8000r / min for 30min, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:5, adjust the pH value to 10, add alkaline lipase A, B compound lipase mixed at 1.5:1, accounting for 0.4% of the aggregate weight ratio, 30°C, hydrolyze 60min, centrifuge, remove the supernatant; superfine pulverized aggregate, Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratio 1: 3:2; the weight volume ratio of aggregate to water is 1:5, add 5% sucrose of the total weight, lactic acid bacteria account for 0.04% by weight of aggregate, ferment at 50°C for 12h, and sterilize at 120°C for 30min; ferment the aggregate Bromelain and papain were a...

Embodiment 2

[0036]Take animal bones and grind them to 5nrn particles, add ethanol, leaching at 60°C for 45min, discard the supernatant; soak and rinse in hot water at 80°C for 30min, centrifuge the aggregate at 4000r / min for 30min, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:3, adjust the pH value to 8, add alkaline lipase A, B mixed at 3:1 compound lipase, accounting for 0.1% of the aggregate weight ratio, 20°C, hydrolyze 40min, centrifuge, remove the supernatant; Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of three kinds of lactic acid bacteria is 1:3:2; aggregate The weight-volume ratio to water is 1:3, add 5% sucrose of the total weight, lactic acid bacteria account for 0.01% by weight of the aggregate, ferment at 35°C for 12h, and sterilize at 120°C for 30min; add bromelain and papaya to the aggregate fermentation liquid A composite ...

Embodiment 3

[0038] Crush animal bones to 20mm particles, add ethanol, extract at 70°C for 60 minutes, discard the supernatant; soak and rinse in hot water at 85°C for 30 minutes, centrifuge the aggregate at 8000r / min for 30 minutes, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:4, adjust the pH value to 9, add alkaline lipase A, B compound lipase mixed at 2:1, accounting for 0.3% of the aggregate weight ratio, 25°C, hydrolyze 50min, centrifuge, and remove the supernatant; Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of three kinds of lactic acid bacteria is 1:3:2; aggregate The weight-volume ratio to water is 1:4, add 5% sucrose of the total weight, lactic acid bacteria account for 0.03% by weight of the aggregate, ferment at 40°C for 12h, and sterilize at 120°C for 30min; add bromelain and papaya to the aggregate fermentation liquid A compo...

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Abstract

The invention discloses an animal bone calcium extraction method and bone gla protein made thereby. The animal bone calcium extraction method includes the steps of triple defatting, double centrifuging, fermenting, and compound protease hydrolysis. Fat in the bone powder made by the defatting process is substantially removed, so that fat content of calcium agent obtained by enzymolysis is smaller than 0.1%. The bone gla protein is safer and healthier for consumers with cardiovascular diseases but causes no increase in financial burden.

Description

technical field [0001] The invention relates to a bone calcium extraction process and belongs to the field of food processing. Background technique [0002] With the aging of society, the prevalence of osteoporosis ranks first among metabolic bone diseases in western countries. The pain that osteoporosis brings to patients and families is self-evident. According to statistics, there are currently more than 100 million osteoporosis patients in China, and it is estimated that the number will increase to 210 million by 2050. Factors causing calcium deficiency in more and more people: Accelerated pace of life and work, high work pressure and irregular life, not paying attention to the reasonable collocation of dietary structure, those with a daily calcium intake of less than 600 mg can be considered as calcium intake Insufficient calcium in the body can easily lead to lack of calcium in the body; some middle-aged people are stressed at work, lack self-care awareness, and do no...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/29A23L1/312A23L33/00A23L13/20
Inventor 黄永亮崔卜东吴益群赵健郁正刚
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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